دورية أكاديمية
Validation of an HPLC-MS/MS method with QuEChERS extraction using isotopic dilution to analyze chlordecone in human serum.
| العنوان: | Validation of an HPLC-MS/MS method with QuEChERS extraction using isotopic dilution to analyze chlordecone in human serum. |
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| المؤلفون: | Saint-Hilaire M; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France. Electronic address: msaint-hilaire@pasteur-guadeloupe.fr., Plumain D; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France., Thomé JP; Université de Liège, LEAE -CART, Freshwater and Oceanic Sciences Unit of Research (FOCUS), B6C, Allée du 6 Août, 11, 4000 Liège, Belgium., Adam C; Université de Liège, LEAE -CART, Freshwater and Oceanic Sciences Unit of Research (FOCUS), B6C, Allée du 6 Août, 11, 4000 Liège, Belgium., Scholl G; Université de Liège, CART, Molecular Systems Unit of Research (MolSys), B6C, Allée du 6 Août, 11, 4000 Liège, Belgium., Vedy S; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France., Ferdinand S; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France., Talarmin A; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France., Guyomard-Rabenirina S; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France. |
| المصدر: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2023 Sep 01; Vol. 1229, pp. 123894. Date of Electronic Publication: 2023 Sep 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101139554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-376X (Electronic) Linking ISSN: 15700232 NLM ISO Abbreviation: J Chromatogr B Analyt Technol Biomed Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| مواضيع طبية MeSH: | Chlordecone*/analysis , Insecticides*/analysis, Humans ; Chromatography, High Pressure Liquid ; Tandem Mass Spectrometry ; Chromatography, Liquid |
| مستخلص: | Until 1993, chlordecone (CLD) was extensively used in banana fields in French West Indies. In a previous study, CLD was detected in 90 % of Martinican and Guadeloupean adult's serum. In order to simplify the analyses of CLD in the serum, a new QuEChERS-HPLC-MS/MS method was implemented and validated by the Pasteur Institute of Guadeloupe (IPG). This method was validated with accuracy profiles according to the French Standard NF V03-110 plus the ISO 15189 and European guidelines. Linearity, repeatability, accuracy, intermediate precision, specificity, limit of detection (LOD), limit of quantification (LOQ) and uncertainty were determined. The accuracy profile allowed the method to be validated between 0.06 µg L -1 and 1.00 µg L -1 of serum. The LOD was 0.02 µg L -1 , the LOQ was 0.06 µg L -1 and the uncertainty of the method was 21 %. A comparison of 49 serum samples between the IPG (LC-MS/MS) and the LEAE-CART (GC-HRMS) laboratories demonstrated that this new method can reliably determine CLD in human serum. Stability tests were performed and duration of the storage of raw samples and extracts before analysis by HPLC-MS/MS. Raw samples were stable after collection for at least one week at 5 °C or 25 °C and for at least 3 months at -20 °C. Extracts in acetonitrile were stable for at least 1 month at -20 °C. These stability results facilitate the daily use of the method. This method should help the entire population of Guadeloupe and Martinique by allowing a routinely analyzed for CLD and will be useful for future projects aimed at improving population health monitoring. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Chlordecone; Human; Method; Serum; Stability; Validation |
| المشرفين على المادة: | RG5XJ88UDF (Chlordecone) 0 (Insecticides) |
| تواريخ الأحداث: | Date Created: 20231014 Date Completed: 20231103 Latest Revision: 20231103 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jchromb.2023.123894 |
| PMID: | 37837856 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-376X |
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| DOI: | 10.1016/j.jchromb.2023.123894 |