دورية أكاديمية
Vaccination against tick-borne encephalitis elicits a detectable NS1 IgG antibody response.
| العنوان: | Vaccination against tick-borne encephalitis elicits a detectable NS1 IgG antibody response. |
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| المؤلفون: | Ackermann-Gäumann R; Microbiologie, ADMED Analyses et Diagnostics Médicaux, La Chaux-de-Fonds, Switzerland; Swiss National Reference Center for Tick-Transmitted Diseases, Switzerland. Electronic address: rahel.ackermann@ne.ch., Brêchet A; Microbiologie, ADMED Analyses et Diagnostics Médicaux, La Chaux-de-Fonds, Switzerland., Smetana J; Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic., Salát J; Laboratory of Emerging Viral Infections, Veterinary Research Institute, Brno, Czech Republic; Laboratory of Arbovirology, Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic., Lienhard R; Microbiologie, ADMED Analyses et Diagnostics Médicaux, La Chaux-de-Fonds, Switzerland; Swiss National Reference Center for Tick-Transmitted Diseases, Switzerland., Croxatto A; Microbiologie, ADMED Analyses et Diagnostics Médicaux, La Chaux-de-Fonds, Switzerland; Swiss National Reference Center for Tick-Transmitted Diseases, Switzerland., Polcarová P; Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic., Chlíbek R; Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic., Růžek D; Laboratory of Emerging Viral Infections, Veterinary Research Institute, Brno, Czech Republic; Laboratory of Arbovirology, Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic; Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic. |
| المصدر: | Journal of virological methods [J Virol Methods] 2023 Dec; Vol. 322, pp. 114831. Date of Electronic Publication: 2023 Oct 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | Encephalitis Viruses, Tick-Borne* , Encephalitis, Tick-Borne*/prevention & control , Flavivirus Infections* , Viral Vaccines*, Humans ; Antibodies, Viral ; Antibody Formation ; Immunoglobulin G ; Vaccination |
| مستخلص: | Vaccine-induced protection against tick-borne encephalitis virus (TBEV) is mediated by antibodies to the viral particle/envelope protein. The detection of non-structural protein 1 (NS1) specific antibodies has been suggested as a marker indicative of natural infections. However, recent work has shown that TBEV vaccines contain traces of NS1, and immunization of mice induced low amounts of NS1-specific antibodies. In this study, we investigated if vaccination induces TBEV NS1-specific antibodies in humans. Healthy army members (n = 898) were asked to fill in a questionnaire relating to flavivirus vaccination or infection, and blood samples were collected. In addition, samples of 71 suspected acute TBE cases were included. All samples were screened for the presence of TBEV NS1-specific IgG antibodies using an in-house developed ELISA. Antibodies were quantified as percent positivity in reference to a positive control. For qualitative evaluation, cut-off for positivity was defined based on the mean OD of the lower 95% of the vaccinated individuals + 3 SD. We found significantly higher NS1-specific IgG antibody titers (i.e., quantitative evaluation) in individuals having received 2, 3, or 4 or more vaccine doses than in non-vaccinated individuals. Similarly, the percentage of individuals with a positive test result (i.e., qualitative evaluation) was higher in individuals vaccinated against tick-borne encephalitis than in unvaccinated study participants. Although NS1-specific IgG titers remained at a relatively low level when compared to TBE patients, a clear distinction was not always possible. Establishing a clear cut-off point in detection systems is critical for NS1-specific antibodies to serve as a marker for distinguishing the immune response after vaccination and infection. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibody; Flavivirus; NS1; Non-structural protein 1; Tick-borne encephalitis; Vaccination |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Immunoglobulin G) 0 (Viral Vaccines) |
| تواريخ الأحداث: | Date Created: 20231014 Date Completed: 20231117 Latest Revision: 20231117 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jviromet.2023.114831 |
| PMID: | 37838083 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0984 |
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| DOI: | 10.1016/j.jviromet.2023.114831 |