دورية أكاديمية
Testing for 2,4-dinitrophenol in human hair by LC-MS/MS and interpretation issues.
| العنوان: | Testing for 2,4-dinitrophenol in human hair by LC-MS/MS and interpretation issues. |
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| المؤلفون: | Kintz P; X-Pertise Consulting, 42 rue principale, 67206 Mittelhausbergen, France; Institut de Médecine légale, 11 rue Humann, 67000 Strasbourg, France. Electronic address: pascal.kintz@wanadoo.fr., Ameline A; Institut de Médecine légale, 11 rue Humann, 67000 Strasbourg, France. |
| المصدر: | Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2024 Jan 05; Vol. 237, pp. 115795. Date of Electronic Publication: 2023 Oct 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 8309336 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-264X (Electronic) Linking ISSN: 07317085 NLM ISO Abbreviation: J Pharm Biomed Anal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2006->: London : Elsevier Science Original Publication: Oxford ; New York : Pergamon Press, c1983- |
| مواضيع طبية MeSH: | 2,4-Dinitrophenol* , Clenbuterol*, Humans ; Chromatography, Liquid/methods ; Tandem Mass Spectrometry/methods ; Hair ; Substance Abuse Detection/methods |
| مستخلص: | 2,4-dinitrophenol, initially developed for industrial purposes, is used in the synthesis of dyes and various drugs, including herbicides, fungicides and insecticides. Incidentally, it was discovered that 2,4-dinitrophenol increases human basal metabolic rate. As a consequence, people who want to loose weight can abuse 2,4-dinitrophenol. It is a compound of choice for bodybuilders who abuse it in combination with anabolic steroids and clenbuterol. Numerous deaths have been attributed to 2,4-dinitrophenol. Up to now, 2,4-dinitrophenol was never reported in hair. The authors present an original method to test for 2,4-dinitrophenol in hair after methanolic extraction using LC-MS/MS. The limit of detection of the procedure is 0.01 ng/mg and the method was fully validated in the range 0.1-50 ng/mg. 2,4-dinitrophenol tested positive in the brown hair of a subject deceased from acute poisoning at 5.15, 13.06 and 25.48 ng/mg in the 3 × 3 cm segments. As excessive sweating is associated to 2,4-dinitrophenol abuse, major contribution of this matrix must be taken into consideration when interpreting the data. In this case, there was a regular increase of 2,4-dinitrophenol concentration from root to tip. This specific pattern can be the consequence of sweat contamination, i.e., the older hair being for a longer time in contact with sweat. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 2; 4-dinitrophenol; Dietary supplements; Doping; Hair; Liquid chromatography |
| المشرفين على المادة: | Q13SKS21MN (2,4-Dinitrophenol) XTZ6AXU7KN (Clenbuterol) |
| تواريخ الأحداث: | Date Created: 20231016 Date Completed: 20231103 Latest Revision: 20231103 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jpba.2023.115795 |
| PMID: | 37844362 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-264X |
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| DOI: | 10.1016/j.jpba.2023.115795 |