دورية أكاديمية
Implementation, Evolution, and Laboratory Performance of Methods-Based Proficiency Testing for Next-Generation Sequencing Detection of Germline Sequence Variants.
| العنوان: | Implementation, Evolution, and Laboratory Performance of Methods-Based Proficiency Testing for Next-Generation Sequencing Detection of Germline Sequence Variants. |
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| المؤلفون: | Tsuchiya KD; From the Department of Laboratory Medicine and Pathology, University of Washington, Seattle (Tsuchiya)., Funke B; Sema4, Stamford, Connecticut, Children's Hospital of Philadelphia, Pennsylvania(Funke)., Hegde M; Revvity Omics, Pittsburgh, Pennsylvania, Children's Hospital of Philadelphia, Pennsylvania(Hegde)., Santani A; Center for Applied Genomics, Children's Hospital of Philadelphia, Pennsylvania (Santani).; LetsGetChecked, Monrovia, California (Santani)., Souers RJ; Biostatistics Department, College of American Pathologists, Northfield, Illinois(Souers)., Szelinger S; Proficiency Testing, College of American Pathologists, Northfield, Illinois(Szelinger, Halley, Zhao, Mot)., Halley J; Proficiency Testing, College of American Pathologists, Northfield, Illinois(Szelinger, Halley, Zhao, Mot)., Zhao Q; Proficiency Testing, College of American Pathologists, Northfield, Illinois(Szelinger, Halley, Zhao, Mot)., Mot N; Proficiency Testing, College of American Pathologists, Northfield, Illinois(Szelinger, Halley, Zhao, Mot)., Roy A; the Departments of Pathology & Immunology and Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston (Roy)., Smith VL; the Department of Pathology, Duke University School of Medicine, Durham, North Carolina (Smith)., Zhang BM; the Department of Pathology, Stanford University School of Medicine, Stanford, California (Zhang)., Voelkerding K; Voelkerding Consulting, Salt Lake City, Utah (Voelkerding)., Moyer AM; the Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota (Moyer). |
| المصدر: | Archives of pathology & laboratory medicine [Arch Pathol Lab Med] 2024 Jul 01; Vol. 148 (7), pp. 775-783. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: College of American Pathologists Country of Publication: United States NLM ID: 7607091 Publication Model: Print Cited Medium: Internet ISSN: 1543-2165 (Electronic) Linking ISSN: 00039985 NLM ISO Abbreviation: Arch Pathol Lab Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Northfield, Ill. : College of American Pathologists Original Publication: Chicago, American Medical Assn. |
| مواضيع طبية MeSH: | Laboratory Proficiency Testing* , High-Throughput Nucleotide Sequencing*/standards , High-Throughput Nucleotide Sequencing*/methods , Germ-Line Mutation*, Humans ; Genetic Testing/methods ; Genetic Testing/standards ; Laboratories, Clinical |
| مستخلص: | Context.—: Next-generation sequencing (NGS)-based assays are used for diagnosis of diverse inherited disorders. Limited data are available pertaining to interlaboratory analytical performance of these assays. Objective.—: To report on the College of American Pathologists (CAP) NGS Germline Program, which is methods based, and explore the evolution in laboratory testing practices. Design.—: Results from the NGS Germline Program from 2016-2020 were analyzed for interlaboratory analytical performance. Self-reported laboratory testing practices were also evaluated. Results.—: From 2016-2020, a total of 297 laboratories participated in at least 1 program mailing. Of the 289 laboratories that provided information on tests offered, 138 (47.8%) offered only panel testing throughout their enrollment, while 35 (12.1%) offered panels and exome testing, 30 (10.4%) offered only exomes, 9 (3.1%) offered only genomes, and 15 (5.2%) offered panels, exomes, and genomes. The remainder (62 laboratories, 21.4%) changed their test offerings during the 2016-2020 timeframe. Considering each genomic position/interval, the median detection percentage at variant positions across the 2016-2020 mailings ranged from 94.3% to 100%, while at reference positions (no variant detected), the median correct response percentage was 100% across all mailings. When considering performance of individual laboratories, 89.5% (136 of 152) to 98.0% (149 of 152) of laboratories successfully met the detection threshold (≥90% of the variants present), while 94.6% (87 of 92) to 100% (163 of 163) of laboratories met the 95% specificity threshold across mailings. Conclusions.—: Since the inception of this program, laboratories have consistently performed well. The median sensitivity and specificity of detection of sequence variants included in this program (eg, single nucleotide variants, insertions, and deletions) were 100.0%. (© 2024 College of American Pathologists.) |
| تواريخ الأحداث: | Date Created: 20231018 Date Completed: 20240625 Latest Revision: 20240625 |
| رمز التحديث: | 20240626 |
| DOI: | 10.5858/arpa.2023-0090-CP |
| PMID: | 37852169 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1543-2165 |
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| DOI: | 10.5858/arpa.2023-0090-CP |