دورية أكاديمية
Recombinant PreS-fusion protein vaccine for birch pollen and apple allergy.
العنوان: | Recombinant PreS-fusion protein vaccine for birch pollen and apple allergy. |
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المؤلفون: | Khaitov M; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation.; Pirogov Russian National Research Medical University, Moscow, Russian Federation., Shilovskiy I; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Valenta R; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation.; Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.; Department of Clinical Immunology and Allergology, Laboratory of Immunopathology, Sechenov First Moscow State Medical University, Moscow, Russian Federation.; Karl Landsteiner University for Healthcare Sciences, Krems, Austria., Weber M; Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Korneev A; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Tulaeva I; Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.; Department of Clinical Immunology and Allergology, Laboratory of Immunopathology, Sechenov First Moscow State Medical University, Moscow, Russian Federation., Gattinger P; Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., van Hage M; Department of Medicine Solna, Division of Immunology and Allergy, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden., Hofer G; Department of Materials and Environmental Chemistry, University of Stockholm, Stockholm, Sweden., Konradsen JR; Department of Women's and Children's Health, Astrid Lindgren Children's Hospital, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden., Keller W; Institute of Molecular Biosciences, BioTechMed Graz, University of Graz, Graz, Austria., Akinfenwa O; Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Poroshina A; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Ilina N; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Fedenko E; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Elisyutina O; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation.; Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation., Litovkina A; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation.; Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation., Smolnikov E; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation.; Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation., Nikonova A; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Rybalkin S; Pirogov Russian National Research Medical University, Moscow, Russian Federation., Aldobaev V; Pirogov Russian National Research Medical University, Moscow, Russian Federation., Smirnov V; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation.; Sechenov First Moscow State Medical University, Moscow, Russian Federation., Shershakova N; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Petukhova O; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Kudlay D; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation.; Sechenov First Moscow State Medical University, Moscow, Russian Federation., Shatilov A; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Timofeeva A; NRC Institute of Immunology, FMBA of Russia, Moscow, Russian Federation., Campana R; Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria., Udin S; Federal State Budgetary Institution 'Centre for Strategic Planning and Management of Biomedical Health Risks' of the Federal Medical Biological Agency, Moscow, Russian Federation., Skvortsova V; Federal Medical Biological Agency of Russia (FMBA Russia), Moscow, Russian Federation. |
المصدر: | Allergy [Allergy] 2024 Apr; Vol. 79 (4), pp. 1001-1017. Date of Electronic Publication: 2023 Oct 19. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: Denmark NLM ID: 7804028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1398-9995 (Electronic) Linking ISSN: 01054538 NLM ISO Abbreviation: Allergy Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Copenhagen : Wiley-Blackwell Original Publication: Copenhagen, Munksgaard. |
مواضيع طبية MeSH: | Malus* , Food Hypersensitivity*/prevention & control, Animals ; Humans ; Rabbits ; Betula ; Recombinant Fusion Proteins ; Pollen ; Escherichia coli ; Antigens, Plant ; Immunoglobulin E ; Allergens ; Vaccines, Synthetic ; Immunoglobulin G ; Plant Proteins |
مستخلص: | Background: IgE cross-sensitization to major birch pollen allergen Bet v 1 and pathogenesis-related (PR10) plant food allergens is responsible for the pollen-food allergy syndrome. Methods: We designed a recombinant protein, AB-PreS, consisting of non-allergenic peptides derived from the IgE-binding sites of Bet v 1 and the cross-reactive apple allergen, Mal d 1, fused to the PreS domain of HBV surface protein as immunological carrier. AB-PreS was expressed in E. coli and purified by chromatography. The allergenic and inflammatory activity of AB-PreS was tested using basophils and PBMCs from birch pollen allergic patients. The ability of antibodies induced by immunization of rabbits with AB-PreS and birch pollen extract-based vaccines to inhibit allergic patients IgE binding to Bet v 1 and Mal d 1 was assessed by ELISA. Results: IgE-binding experiments and basophil activation test revealed the hypoallergenic nature of AB-PreS. AB-PreS induced lower T-cell activation and inflammatory cytokine production in cultured PBMCs from allergic patients. IgG antibodies induced by five injections with AB-PreS inhibited allergic patients' IgE binding to Bet v 1 and Mal d 1 better than did IgG induced by up to 30 injections of six licensed birch pollen allergen extract-based vaccines. Additionally, immunization with AB-PreS induced HBV-specific antibodies potentially protecting from infection with HBV. Conclusion: The recombinant AB-PreS-based vaccine is hypoallergenic and superior over currently registered allergen extract-based vaccines regarding the induction of blocking antibodies to Bet v 1 and Mal d 1 in animals. (© 2023 European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.) |
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معلومات مُعتمدة: | Austrian Science Fund; Megagrants |
فهرسة مساهمة: | Keywords: allergen; allergen‐specific immunotherapy; birch pollen‐associated food allergy syndrome; molecular allergy vaccine |
المشرفين على المادة: | 0 (Recombinant Fusion Proteins) 0 (Antigens, Plant) 37341-29-0 (Immunoglobulin E) 0 (Allergens) 0 (Vaccines, Synthetic) 0 (Immunoglobulin G) 0 (Plant Proteins) |
تواريخ الأحداث: | Date Created: 20231019 Date Completed: 20240401 Latest Revision: 20240710 |
رمز التحديث: | 20240710 |
DOI: | 10.1111/all.15919 |
PMID: | 37855043 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1398-9995 |
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DOI: | 10.1111/all.15919 |