دورية أكاديمية
أعرض في EDS

Assessing shared respiratory pathogens between domestic (Ovis aries) and bighorn (Ovis canadensis) sheep; methods for multiplex PCR, amplicon sequencing, and bioinformatics to characterize respiratory flora.

التفاصيل البيبلوغرافية
العنوان: Assessing shared respiratory pathogens between domestic (Ovis aries) and bighorn (Ovis canadensis) sheep; methods for multiplex PCR, amplicon sequencing, and bioinformatics to characterize respiratory flora.
المؤلفون: Fox KA; Wildlife Health Program, Colorado Parks and Wildlife, Fort Collins, Colorado, United States of America., MacGlover CAW; Department of Veterinary Sciences, University of Wyoming, Laramie, Wyoming, United States of America., Blecha KA; Terrestrial Branch, Colorado Parks and Wildlife, Gunnison, Colorado, United States of America., Stenglein MD; Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, United States of America.
المصدر: PloS one [PLoS One] 2023 Oct 19; Vol. 18 (10), pp. e0293062. Date of Electronic Publication: 2023 Oct 19 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Sheep, Bighorn*/genetics , Sheep, Bighorn*/microbiology , Sheep Diseases*/epidemiology , Mannheimia haemolytica*/genetics , Respiratory Tract Diseases*, Animals ; Sheep ; Sheep, Domestic ; Multiplex Polymerase Chain Reaction ; RNA, Ribosomal, 16S/genetics ; Computational Biology
مستخلص: Respiratory disease is responsible for dramatic population declines in bighorn sheep (Ovis canadensis), and respiratory pathogen diagnostics contribute to the management of bighorn populations. To create a comprehensive and consistent approach to bighorn sheep respiratory diagnostics, we created a culture-independent assay to detect and strain type Mannheimia haemolytica, Bibersteinia trehalosi, Pasteurella multocida, and Mycoplasma ovipneumoniae. The assay also detects and characterizes the Pasteurellaceae leukotoxin A gene, and broadly assesses the bacterial composition of each sample based on 16S rRNA sequences. The assay is based on a three-step approach: 1) Multiplex PCR to amplify targets including eight loci for each bacterial species, the Pasteurellaceae lktA gene, and the 16S rRNA gene 2) Library preparation, barcoding, and short-read Illumina sequencing to determine the genetic sequences of each target, and 3) Bioinformatics in the form of automated software to analyze genetic sequences. The assay was designed to assess shared pathogens between domestic and bighorn sheep, but could be useful for many applications in bighorn sheep respiratory disease research and management.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Fox et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (RNA, Ribosomal, 16S)
تواريخ الأحداث: Date Created: 20231019 Date Completed: 20231023 Latest Revision: 20231025
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10586700
DOI: 10.1371/journal.pone.0293062
PMID: 37856492
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0293062