دورية أكاديمية
Protocol for assessing translation in living Drosophila imaginal discs by O-propargyl-puromycin incorporation.
| العنوان: | Protocol for assessing translation in living Drosophila imaginal discs by O-propargyl-puromycin incorporation. |
|---|---|
| المؤلفون: | Kiparaki M; Institute for Fundamental Biomedical Research, Biomedical Sciences Research Center 'Alexander Fleming', 16672 Vari, Attiki, Greece. Electronic address: kiparaki@fleming.gr., Baker NE; Department of Genetics, Albert Einstein College of Medicine, The Bronx, NY 10461, USA; Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, The Bronx, NY 10461, USA; Department of Opthalmology and Visual Sciences, Albert Einstein College of Medicine, The Bronx, NY 10461, USA. Electronic address: nicholas.baker@einstein.med.org. |
| المصدر: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102653. Date of Electronic Publication: 2023 Oct 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Drosophila* , Imaginal Discs*, Puromycin/*analogs & derivatives, Animals ; Larva/metabolism ; Puromycin/pharmacology |
| مستخلص: | Translation is a fundamental process of cellular behavior. Here, we present a protocol for measuring translation in Drosophila epithelial tissues using O-propargyl-puromycin (OPP), a puromycin derivative. We detail steps for larval dissection, OPP incorporation, fixation, OPP labeling, immunostaining, and imaging. We also provide details of quantification analysis. Significantly, OPP addition to methionine-containing media enables polypeptide labeling in living cells. Here, we study wing imaginal discs, an excellent model system for investigating growth, proliferation, pattern formation, differentiation, and cell death. For complete details on the use and execution of this protocol, please refer to Lee et al. (2018), Ji et al. (2019), and Kiparaki et al. (2022). 1 , 2 , 3 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | P30 CA013330 United States CA NCI NIH HHS; P40 OD018537 United States OD NIH HHS; R01 GM120451 United States GM NIGMS NIH HHS; S10 OD023591 United States OD NIH HHS |
| فهرسة مساهمة: | Keywords: Developmental biology; Genetics; Model Organisms; Molecular/Chemical Probes |
| المشرفين على المادة: | 0 (O-propargyl-puromycin) 4A6ZS6Q2CL (Puromycin) |
| تواريخ الأحداث: | Date Created: 20231020 Date Completed: 20240311 Latest Revision: 20240311 |
| رمز التحديث: | 20240311 |
| مُعرف محوري في PubMed: | PMC10616417 |
| DOI: | 10.1016/j.xpro.2023.102653 |
| PMID: | 37862174 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
|---|---|
| DOI: | 10.1016/j.xpro.2023.102653 |