دورية أكاديمية
Metagenomic insights into the microbial cooperative networks of a benz(a)anthracene-7,12-dione degrading community from a creosote-contaminated soil.
| العنوان: | Metagenomic insights into the microbial cooperative networks of a benz(a)anthracene-7,12-dione degrading community from a creosote-contaminated soil. |
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| المؤلفون: | Jiménez-Volkerink SN; Department of Genetics, Microbiology and Statistics, University of Barcelona, Av. Diagonal, 643, 08028 Barcelona, Spain., Jordán M; Department of Genetics, Microbiology and Statistics, University of Barcelona, Av. Diagonal, 643, 08028 Barcelona, Spain., Smidt H; Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708, WE, Wageningen, the Netherlands., Minguillón C; Department of Nutrition, Food Science and Gastronomy, University of Barcelona, Avda. Prat de la Riba, 171, 08921 Sta. Coloma de Gramanet, Barcelona, Spain., Vila J; Department of Genetics, Microbiology and Statistics, University of Barcelona, Av. Diagonal, 643, 08028 Barcelona, Spain. Electronic address: qvila@ub.edu., Grifoll M; Department of Genetics, Microbiology and Statistics, University of Barcelona, Av. Diagonal, 643, 08028 Barcelona, Spain. |
| المصدر: | The Science of the total environment [Sci Total Environ] 2024 Jan 10; Vol. 907, pp. 167832. Date of Electronic Publication: 2023 Oct 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0330500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1026 (Electronic) Linking ISSN: 00489697 NLM ISO Abbreviation: Sci Total Environ Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier. |
| مواضيع طبية MeSH: | Polycyclic Aromatic Hydrocarbons*/metabolism , Soil Pollutants*/metabolism, Microbial Consortia ; Creosote ; Sand ; Biodegradation, Environmental ; Carbon ; Soil Microbiology ; Soil |
| مستخلص: | Genotoxicity of PAH-contaminated soils can eventually increase after bioremediation due to the formation and accumulation of polar transformation products, mainly oxygenated PAHs (oxy-PAHs). Biodegradation of oxy-PAHs has been described in soils, but information on the microorganisms and mechanisms involved is still scarce. Benz(a)anthracene-7,12-dione (BaAQ), a transformation product from benz(a)anthracene frequently detected in soils, presents higher genotoxic potential than its parent PAH. Here, using sand-in-liquid microcosms we identified a specialized BaAQ-degrading subpopulation in a PAH-contaminated soil. A BaAQ-degrading microbial consortium was obtained by enrichment in sand-in-liquid cultures with BaAQ as sole carbon source, and its metagenomic analysis identified members of Sphingobium, Stenotrophomonas, Pusillimonas, Olivibacter, Pseudomonas, Achromobacter, and Hyphomicrobiales as major components. The integration of data from metabolomic and metagenomic functional gene analyses of the consortium revealed that the BaAQ metabolic pathway was initiated by Baeyer-Villiger monooxygenases (BVMOs). The presence of plasmid pANTQ-1 in the metagenomic sequences, identified in a previous multi-omic characterization of a 9,10-anthraquinone-degrading isolate recovered from the same soil, suggested the occurrence of a horizontal gene transfer event. Further metagenomic analysis of the BaAQ-degrading consortium also provided insights into the potential roles and interactions within the consortium members. Several potential auxotrophies were detected, indicating that relevant nutritional interdependencies and syntrophic associations were taking place within the community members, not only to provide suitable carbon and energy sources, but also to supply essential nutrients and cofactors. Our work confirms the essential role that BVMO may play as a detoxification mechanism to mitigate the risk posed by oxy-PAH formation during bioremediation of contaminated soils. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 7,12-benz(a)anthraquinone; Bacterial consortium; Baeyer-Villiger; Horizontal gene transfer; Microbial interactions; Oxy-PAHs |
| المشرفين على المادة: | BL89U5YZBK (7,12-benz(a)anthraquinone) 8021-39-4 (Creosote) 0 (Sand) 0 (Polycyclic Aromatic Hydrocarbons) EH46A1TLD7 (anthracene) 7440-44-0 (Carbon) 0 (Soil Pollutants) 0 (Soil) |
| تواريخ الأحداث: | Date Created: 20231020 Date Completed: 20231122 Latest Revision: 20231122 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.scitotenv.2023.167832 |
| PMID: | 37863223 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1026 |
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| DOI: | 10.1016/j.scitotenv.2023.167832 |