Sequential gentle hydration increases encapsulation in model protocells.
| العنوان: | Sequential gentle hydration increases encapsulation in model protocells. |
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| المؤلفون: | Gehlbach EM; University of Minnesota Department of Genetics, Cell Biology and Development, Minneapolis, MN, USA., Robinson AO; University of Minnesota Department of Genetics, Cell Biology and Development, Minneapolis, MN, USA., Engelhart AE; University of Minnesota Department of Genetics, Cell Biology and Development, Minneapolis, MN, USA., Adamala KP; University of Minnesota Department of Genetics, Cell Biology and Development, Minneapolis, MN, USA. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2023 Oct 15. Date of Electronic Publication: 2023 Oct 15. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | Small, spherical vesicles are a widely used chassis for the formation of model protocells and investigating the beginning of compartmentalized evolution. Various methods exist for their preparation, with one of the most common approaches being gentle hydration, where thin layers of lipids are hydrated with aqueous solutions and gently agitated to form vesicles. An important benefit to gentle hydration is that the method produces vesicles without introducing any organic contaminants, such as mineral oil, into the lipid bilayer. However, compared to other methods of liposome formation, gentle hydration is much less efficient at encapsulating aqueous cargo. Improving the encapsulation efficiency of gentle hydration would be of broad use for medicine, biotechnology, and protocell research. Here, we describe a method of sequentially hydrating lipid thin films to increase encapsulation efficiency. We demonstrate that sequential gentle hydration significantly improves encapsulation of water-soluble cargo compared to the traditional method, and that this improved efficiency is dependent on buffer composition. Similarly, we also demonstrate how this method can be used to increase concentrations of oleic acid, a fatty acid commonly used in origins of life research, to improve the formation of vesicles in aqueous buffer. |
| التعليقات: | Update in: Discov Life. 2024;54(1):2. (PMID: 38765272) |
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| معلومات مُعتمدة: | T32 GM008347 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: encapsulation; gentle hydration; liposomes; origins of life; protocells; synthetic biology; synthetic cells; vesicles |
| تواريخ الأحداث: | Date Created: 20231024 Latest Revision: 20240603 |
| رمز التحديث: | 20240603 |
| مُعرف محوري في PubMed: | PMC10592796 |
| DOI: | 10.1101/2023.10.15.562404 |
| PMID: | 37873423 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2023.10.15.562404 |
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