دورية أكاديمية
Characterization of thraustochytrid-specific sterol O -acyltransferase: modification of DGAT2-like enzyme to increase the sterol production in Aurantiochytrium limacinum mh0186.
العنوان: | Characterization of thraustochytrid-specific sterol O -acyltransferase: modification of DGAT2-like enzyme to increase the sterol production in Aurantiochytrium limacinum mh0186. |
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المؤلفون: | Ishibashi Y; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan., Sadamitsu S; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan., Fukahori Y; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan., Yamamoto Y; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan., Tanogashira R; Kyushu University Future Creators in Science Project (QFC-SP) , Fukuoka, Japan., Watanabe T; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan., Hayashi M; Department of Marine Biology and Environmental Sciences, Faculty of Agriculture, University of Miyazaki , Miyazaki, Japan., Ito M; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan., Okino N; Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka, Japan. |
المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2023 Nov 29; Vol. 89 (11), pp. e0100123. Date of Electronic Publication: 2023 Oct 24. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
مواضيع طبية MeSH: | Sterols* , Sterol O-Acyltransferase*, Lipid Droplets ; Vitamin D ; Diacylglycerol O-Acyltransferase/genetics |
مستخلص: | Importance: Since the global market for sterols and vitamin D are grown with a high compound annual growth rate, a sustainable source of these compounds is required to keep up with the increasing demand. Thraustochytrid is a marine oleaginous microorganism that can synthesize several sterols, which are stored as SE in lipid droplets. DGAT2C is an unconventional SE synthase specific to thraustochytrids. Although the primary structure of DGAT2C shows high similarities with that of DGAT, DGAT2C utilizes sterol as an acceptor substrate instead of diacylglycerol. In this study, we examined more detailed enzymatic properties, intracellular localization, and structure-activity relationship of DGAT2C. Furthermore, we successfully developed a method to increase sterol and provitamin D3 productivity of thraustochytrid by more than threefold in the process of elucidating the function of the DGAT2C-specific N-terminal region. Our findings could lead to sustainable sterol and vitamin D production using thraustochytrid. Competing Interests: The authors declare no conflict of interest. |
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معلومات مُعتمدة: | JP15K18740, 21K06034 MEXT | Japan Society for the Promotion of Science (JSPS); JST Global Science Campus MEXT | Japan Science and Technology Agency (JST) |
فهرسة مساهمة: | Keywords: DGAT; diacylglycerol; sterol ester; sterol o-acyltransferase; sterols; thraustochytrid |
المشرفين على المادة: | 0 (Sterols) EC 2.3.1.26 (Sterol O-Acyltransferase) 1406-16-2 (Vitamin D) EC 2.3.1.20 (Diacylglycerol O-Acyltransferase) |
تواريخ الأحداث: | Date Created: 20231024 Date Completed: 20231130 Latest Revision: 20231220 |
رمز التحديث: | 20231221 |
مُعرف محوري في PubMed: | PMC10686087 |
DOI: | 10.1128/aem.01001-23 |
PMID: | 37874286 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1098-5336 |
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DOI: | 10.1128/aem.01001-23 |