دورية أكاديمية
أعرض في EDS

Development and qualification of clinical grade decellularized and cryopreserved human esophagi.

التفاصيل البيبلوغرافية
العنوان: Development and qualification of clinical grade decellularized and cryopreserved human esophagi.
المؤلفون: Godefroy W; Service de Chirurgie Viscérale, Cancérologique et Endocrinienne, Hôpital Saint-Louis - Université Paris Cité, Paris, France. william.godefroy@aphp.fr.; Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France. william.godefroy@aphp.fr.; CIC de Biothérapies CBT 501, Paris, France. william.godefroy@aphp.fr.; Human Immunology, Pathophysiology, Immunotherapy / HIPI / INSERM UMR976, Laboratoire de Biotechnologies de Cellules Souches, Université Paris Cité, 75010, Paris, France. william.godefroy@aphp.fr., Faivre L; Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France.; CIC de Biothérapies CBT 501, Paris, France.; Human Immunology, Pathophysiology, Immunotherapy / HIPI / INSERM UMR976, Laboratoire de Biotechnologies de Cellules Souches, Université Paris Cité, 75010, Paris, France., Sansac C; Banque de Tissus Humains, Hôpital St-Louis, AP-HP, Paris, France., Thierry B; Human Immunology, Pathophysiology, Immunotherapy / HIPI / INSERM UMR976, Laboratoire de Biotechnologies de Cellules Souches, Université Paris Cité, 75010, Paris, France.; Service d'ORL Pédiatrique, AP-HP, Hôpital Universitaire Necker, 75015, Paris, France., Allain JM; LMS, CNRS, Ecole Polytechnique, Institut Polytechnique de Paris, Palaiseau, France.; Inria, Paris, France., Bruneval P; Service d'Anatomie Pathologie, Hôpital Européen Georges Pompidou, AP-HP, Paris, France., Agniel R; Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe (EA1391), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris Université, Cergy-Pontoise, France., Kellouche S; Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, ERRMECe (EA1391), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris Université, Cergy-Pontoise, France., Monasson O; CNRS, BioCIS, CY Cergy Paris Université, 95000, Cergy Pontoise, France.; CNRS, BioCIS, Université Paris-Saclay, 92290, Châtenay-Malabry, France., Peroni E; CNRS, BioCIS, CY Cergy Paris Université, 95000, Cergy Pontoise, France.; CNRS, BioCIS, Université Paris-Saclay, 92290, Châtenay-Malabry, France., Jarraya M; Banque de Tissus Humains, Hôpital St-Louis, AP-HP, Paris, France., Setterblad N; UMS Saint-Louis US53 / UAR2030, Institut de Recherche Saint-Louis Plateforme Technologique Centre, Université Paris Cité - Inserm - CNRS, Paris, France., Braik M; Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France., Even B; Laboratoire Gly-CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France., Cheverry S; Laboratoire Gly-CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France., Domet T; Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France.; CIC de Biothérapies CBT 501, Paris, France., Albanese P; Laboratoire Gly-CRRET, Université Paris Est Créteil, Université Paris Est, EA 4397 ERL CNRS 9215, Créteil, France., Larghero J; Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France.; CIC de Biothérapies CBT 501, Paris, France.; Human Immunology, Pathophysiology, Immunotherapy / HIPI / INSERM UMR976, Laboratoire de Biotechnologies de Cellules Souches, Université Paris Cité, 75010, Paris, France.; Centre MEARY de Thérapie Cellulaire Et Génique, AP-HP, Hôpital Saint-Louis, 75010, Paris, France., Cattan P; Service de Chirurgie Viscérale, Cancérologique et Endocrinienne, Hôpital Saint-Louis - Université Paris Cité, Paris, France.; Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France.; CIC de Biothérapies CBT 501, Paris, France.; Human Immunology, Pathophysiology, Immunotherapy / HIPI / INSERM UMR976, Laboratoire de Biotechnologies de Cellules Souches, Université Paris Cité, 75010, Paris, France., Arakelian L; Unité de Thérapie Cellulaire, Hôpital Saint-Louis, AP-HP, Paris, France. lousineh.arakelian@aphp.fr.; CIC de Biothérapies CBT 501, Paris, France. lousineh.arakelian@aphp.fr.; Human Immunology, Pathophysiology, Immunotherapy / HIPI / INSERM UMR976, Laboratoire de Biotechnologies de Cellules Souches, Université Paris Cité, 75010, Paris, France. lousineh.arakelian@aphp.fr.
المصدر: Scientific reports [Sci Rep] 2023 Oct 25; Vol. 13 (1), pp. 18283. Date of Electronic Publication: 2023 Oct 25.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Tissue Scaffolds*/chemistry , Extracellular Matrix*, Mice ; Animals ; Humans ; Tissue Engineering/methods ; Cryopreservation ; Sodium Dodecyl Sulfate/chemistry ; Esophagus
مستخلص: Tissue engineering is a promising alternative to current full thickness circumferential esophageal replacement methods. The aim of our study was to develop a clinical grade Decellularized Human Esophagus (DHE) for future clinical applications. After decontamination, human esophagi from deceased donors were placed in a bioreactor and decellularized with sodium dodecyl sulfate (SDS) and ethylendiaminetetraacetic acid (EDTA) for 3 days. The esophagi were then rinsed in sterile water and SDS was eliminated by filtration on an activated charcoal cartridge for 3 days. DNA was removed by a 3-hour incubation with DNase. A cryopreservation protocol was evaluated at the end of the process to create a DHE cryobank. The decellularization was efficient as no cells and nuclei were observed in the DHE. Sterility of the esophagi was obtained at the end of the process. The general structure of the DHE was preserved according to immunohistochemical and scanning electron microscopy images. SDS was efficiently removed, confirmed by a colorimetric dosage, lack of cytotoxicity on Balb/3T3 cells and mesenchymal stromal cell long term culture. Furthermore, DHE did not induce lymphocyte proliferation in-vitro. The cryopreservation protocol was safe and did not affect the tissue, preserving the biomechanical properties of the DHE. Our decellularization protocol allowed to develop the first clinical grade human decellularized and cryopreserved esophagus.
(© 2023. Springer Nature Limited.)
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المشرفين على المادة: 368GB5141J (Sodium Dodecyl Sulfate)
تواريخ الأحداث: Date Created: 20231025 Date Completed: 20231027 Latest Revision: 20231122
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10600094
DOI: 10.1038/s41598-023-45610-5
PMID: 37880340
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-45610-5