دورية أكاديمية
Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes.
| العنوان: | Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes. |
|---|---|
| المؤلفون: | de Mello Seal DC; Laboratorio de Andrologia, Departamento de Reproducao Animal, Universidade Federal Rural de Pernambuco. Recife, Brasil. desiree.seal@hotmail.com., Monteiro M; Laboratorio de Andrologia, Departamento de Reproducao Animal, Universidade Federal Rural de Pernambuco. Recife, Brasil. desiree.seal@hotmail.com., Pereira Arruda LC; Laboratorio de Andrologia, Departamento de Reproducao Animal, Universidade Federal Rural de Pernambuco. Recife, Brasil. desiree.seal@hotmail.com., de Souza JH; Laboratorio de Andrologia, Departamento de Reproducao Animal, Universidade Federal Rural de Pernambuco. Recife, Brasil. desiree.seal@hotmail.com., de Vasconcelos Alves RR; Laboratorio de Bioquimica de Proteinas, Departamento de Bioquimica, Universidade Federal de Pernambuco, Recife, Brasil., Lira Soares LA; Laboratorio de Bioquimica de Proteinas, Departamento de Bioquimica, Universidade Federal de Pernambuco, Recife, Brasil., Napoleao TH; Laboratorio de Bioquimica de Proteinas, Departamento de Bioquimica, Universidade Federal de Pernambuco, Recife, Brasil., Guedes Paiva PM; Laboratorio de Bioquimica de Proteinas, Departamento de Bioquimica, Universidade Federal de Pernambuco, Recife, Brasil., Bezerra de Lima PE; Laboratorio de Microbiologia e Biologia Celular, Departamento de Microbiologia, Instituto Aggeu Magalhaes - Fiocruz, Recife, Brasil., Bressan Queiroz de Figueiredo RC; Laboratorio de Microbiologia e Biologia Celular, Departamento de Microbiologia, Instituto Aggeu Magalhaes - Fiocruz, Recife, Brasil., Pessoa Guerra MM; Laboratorio de Microbiologia e Biologia Celular, Departamento de Microbiologia, Instituto Aggeu Magalhaes - Fiocruz, Recife, Brasil. |
| المصدر: | Cryo letters [Cryo Letters] 2023 May-Jun; Vol. 44 (3), pp. 151-159. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: CryoLetters Country of Publication: England NLM ID: 9891832 Publication Model: Print Cited Medium: Print ISSN: 0143-2044 (Print) Linking ISSN: 01432044 NLM ISO Abbreviation: Cryo Letters Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, Eng. : CryoLetters Original Publication: [London : Royal Veterinary College, |
| مواضيع طبية MeSH: | Moringa oleifera* , Semen Preservation*/veterinary, Male ; Animals ; Freezing ; Goats ; Cryopreservation/veterinary ; Sperm Motility ; Seeds ; Spermatozoa ; Egg Yolk/chemistry ; Cryoprotective Agents/pharmacology |
| مستخلص: | Background: Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant. Objective: The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency. Materials and Methods: Ejaculates (n=6) from four goat breeders were pooled and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed. Results: With the SM extender, straightness, wobble and plasma membrane integrity were lower than in the control group (P < 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (P < 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used, damaged the membrane of sperm cells in a dose-dependent manner. Conclusion: The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner. DOI: 10.54680/fr23310110712. |
| المشرفين على المادة: | 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20231026 Date Completed: 20231102 Latest Revision: 20231102 |
| رمز التحديث: | 20231102 |
| PMID: | 37883168 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0143-2044 |
|---|