دورية أكاديمية
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Immunotechniques for the Group Determination of Macrolide Antibiotics Traces in the Environment Using a Volume-Mediated Sensitivity Enhancement Strategy.

التفاصيل البيبلوغرافية
العنوان: Immunotechniques for the Group Determination of Macrolide Antibiotics Traces in the Environment Using a Volume-Mediated Sensitivity Enhancement Strategy.
المؤلفون: Burkin MA; I. Mechnikov Research Institute for Vaccines and Sera, 105064 Moscow, Russia., Tevyashova AN; Gause Institute of New Antibiotics, 199021 Moscow, Russia.; School of Science, Constructor University, 28759 Bremen, Germany., Bychkova EN; Gause Institute of New Antibiotics, 199021 Moscow, Russia., Melekhin AO; Department of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.; Federal Centre for Animal Health, 111622 Moscow, Russia., Galvidis IA; I. Mechnikov Research Institute for Vaccines and Sera, 105064 Moscow, Russia.
المصدر: Biosensors [Biosensors (Basel)] 2023 Oct 10; Vol. 13 (10). Date of Electronic Publication: 2023 Oct 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Pub.
مواضيع طبية MeSH: Azithromycin*/analysis , Tandem Mass Spectrometry*, Humans ; Anti-Bacterial Agents/analysis ; Macrolides ; Erythromycin/analysis ; Haptens ; Water
مستخلص: Macrolide antibiotics, which are effective antimicrobial agents, are intensively used in human and veterinary medicine, as well as in agriculture. Consequently, they are found all over the world as environmental pollutants, causing harm to sensitive ecological communities and provoking a selection of resistant forms. A novel azithromycin derivative, which was used as hapten conjugate, ensured the group immunorecognition of six major macrolide representatives (105-41%), namely erythromycin, erythromycin ethylsuccinate, clarithromycin, roxithromycin, azithromycin, and dirithromycin in a competitive immunoassay based on anti-clarithromycin antibodies. The heterologous hapten-based ELISA format resulted in a 5-fold increase in sensitivity, with an IC 50 value of 0.04 ng/mL for erythromycin. In this study, we proposed an underexploited strategy in an immunoassay field to significantly improve the detectability of analytes in environmental samples. Unlike most approaches, it does not require special enhancers/amplifiers or additional concentration/extraction procedures; instead, it involves analyzing a larger volume of test samples. A gradual volume increase in the samples (from 0.025 to 10 mL) analyzed using a direct competitive ELISA, immunobeads, and immunofiltration assay formats based on the same reagents resulted in a significant improvement (more than 50-fold) in assay sensitivity and detection limit up to 5 and 1 pg/mL, respectively. The suitability of the test for detecting the macrolide contamination of natural water was confirmed by the recovery of macrolides from spiked blank samples (71.7-141.3%). During 2022-2023, a series of natural water samples from Lake Onega and its influents near Petrozavodsk were analyzed, using both the developed immunoassay and HPLC-MS/MS. The results revealed no contamination of macrolide antibiotic.
References: Anal Bioanal Chem. 2015 Dec;407(30):9125-33. (PMID: 26497839)
Environ Toxicol Chem. 2008 May;27(5):1201-8. (PMID: 18419195)
Biosensors (Basel). 2019 Apr 04;9(2):. (PMID: 30987327)
J Pharm Biomed Anal. 2021 Sep 10;204:114275. (PMID: 34311285)
Electrophoresis. 2012 Oct;33(19-20):2933-52. (PMID: 23019127)
J Immunol Methods. 2013 Feb 28;388(1-2):60-7. (PMID: 23234756)
Eur J Med Chem. 2013 Jan;59:209-17. (PMID: 23229056)
Anal Chem. 2018 Apr 3;90(7):4901-4908. (PMID: 29533661)
Anal Biochem. 2015 Jan 1;468:75-82. (PMID: 25256165)
Vet Ther. 2005 Summer;6(2):83-95. (PMID: 16094557)
J Hazard Mater. 2022 Oct 5;439:129628. (PMID: 35905608)
Expert Rev Anti Infect Ther. 2021 Feb;19(2):147-163. (PMID: 32853038)
Eur J Pharmacol. 2020 Jan 5;866:172813. (PMID: 31751574)
Int J Antimicrob Agents. 2018 Mar;51(3):283-298. (PMID: 28668674)
Prog Med Chem. 1993;30:57-88. (PMID: 8303037)
Anal Chem. 2020 Jan 7;92(1):473-505. (PMID: 31825597)
J Immunol Methods. 1990 Mar 27;128(1):9-16. (PMID: 2324507)
Anal Chem. 2011 Mar 15;83(6):1969-76. (PMID: 21338074)
Anal Chim Acta. 2022 Jan 2;1189:338907. (PMID: 34815045)
Chemosphere. 2023 Mar;316:137793. (PMID: 36640977)
Foods. 2023 Apr 07;12(8):. (PMID: 37107376)
Ecotoxicology. 2022 Sep;31(7):1111-1119. (PMID: 35841472)
J Pharm Biomed Anal. 2020 Sep 10;189:113450. (PMID: 32693204)
J Hazard Mater. 2015;283:778-86. (PMID: 25464321)
Food Chem. 2015 Mar 15;171:98-107. (PMID: 25308648)
فهرسة مساهمة: Keywords: enzyme-linked immunosorbent assay; group recognition; hapten design; immunobeads assay; immunofiltration; macrolide antibiotics
المشرفين على المادة: 83905-01-5 (Azithromycin)
0 (Anti-Bacterial Agents)
0 (Macrolides)
63937KV33D (Erythromycin)
0 (Haptens)
059QF0KO0R (Water)
تواريخ الأحداث: Date Created: 20231027 Date Completed: 20231030 Latest Revision: 20231030
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10605010
DOI: 10.3390/bios13100921
PMID: 37887114
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6374
DOI:10.3390/bios13100921