دورية أكاديمية
أعرض في EDS

Antibacterial and Anti-Efflux Activities of Cinnamon Essential Oil against Pan and Extensive Drug-Resistant Pseudomonas aeruginosa Isolated from Human and Animal Sources.

التفاصيل البيبلوغرافية
العنوان: Antibacterial and Anti-Efflux Activities of Cinnamon Essential Oil against Pan and Extensive Drug-Resistant Pseudomonas aeruginosa Isolated from Human and Animal Sources.
المؤلفون: Abdelatti MAI; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Abd El-Aziz NK; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., El-Naenaeey EYM; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Ammar AM; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt., Alharbi NK; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia., Alharthi A; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi Arabia., Zakai SA; Department of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia., Abdelkhalek A; Food Safety, Hygiene and Technology Department, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Badr City 11829, Egypt.
المصدر: Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2023 Oct 05; Vol. 12 (10). Date of Electronic Publication: 2023 Oct 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101637404 Publication Model: Electronic Cited Medium: Print ISSN: 2079-6382 (Print) Linking ISSN: 20796382 NLM ISO Abbreviation: Antibiotics (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, 2012-
مستخلص: Pseudomonas aeruginosa is notorious for its ability to develop a high level of resistance to antimicrobial agents. Resistance-nodulation-division (RND) efflux pumps could mediate drug resistance in P. aeruginosa . The present study aimed to evaluate the antibacterial and anti-efflux activities of cinnamon essential oil either alone or combined with ciprofloxacin against drug resistant P. aeruginosa originated from human and animal sources. The results revealed that 73.91% of the examined samples were positive for P. aeruginosa ; among them, 77.78% were of human source and 72.73% were recovered from animal samples. According to the antimicrobial resistance profile, 48.73% of the isolates were multidrug-resistant (MDR), 9.2% were extensive drug-resistant (XDR), and 0.84% were pan drug-resistant (PDR). The antimicrobial potential of cinnamon oil against eleven XDR and one PDR P. aeruginosa isolates was assessed by the agar well diffusion assay and broth microdilution technique. The results showed strong antibacterial activity of cinnamon oil against all tested P. aeruginosa isolates with inhibition zones' diameters ranging from 34 to 50 mm. Moreover, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of cinnamon oil against P. aeruginosa isolates ranged from 0.0562-0.225 µg/mL and 0.1125-0.225 µg/mL, respectively. The cinnamon oil was further used to evaluate its anti-efflux activity against drug-resistant P. aeruginosa by phenotypic and genotypic assays. The cartwheel test revealed diminished efflux pump activity post cinnamon oil exposure by two-fold indicating its reasonable impact. Moreover, the real-time quantitative polymerase chain reaction (RT-qPCR) results demonstrated a significant ( p < 0.05) decrease in the expression levels of MexA and MexB genes of P. aeruginosa isolates treated with cinnamon oil when compared to the non-treated ones (fold changes values ranged from 0.4204-0.7474 for MexA and 0.2793-0.4118 for MexB ). In conclusion, we suggested the therapeutic use of cinnamon oil as a promising antibacterial and anti-efflux agent against drug-resistant P. aeruginosa .
References: Nat Prod Res. 2012;26(23):2235-8. (PMID: 22191514)
J Adv Pharm Technol Res. 2017 Jul-Sep;8(3):86-90. (PMID: 28795021)
Eur J Clin Microbiol Infect Dis. 2012 Oct;31(10):2791-7. (PMID: 22552893)
Chem Rev. 2017 Oct 11;117(19):12415-12474. (PMID: 28953368)
Future Microbiol. 2020 Jan;15:143-157. (PMID: 32073314)
Clin Microbiol Infect. 2012 Mar;18(3):268-81. (PMID: 21793988)
Antibiotics (Basel). 2023 Jan 15;12(1):. (PMID: 36671381)
Essays Biochem. 2017 Mar 3;61(1):49-59. (PMID: 28258229)
Microb Pathog. 2018 Jul;120:198-203. (PMID: 29702210)
Clin Microbiol Rev. 2012 Oct;25(4):661-81. (PMID: 23034325)
BMC Complement Altern Med. 2016 Jun 01;16:158. (PMID: 27245046)
Clin Microbiol Rev. 2015 Apr;28(2):337-418. (PMID: 25788514)
J Glob Infect Dis. 2016 Oct-Dec;8(4):155-159. (PMID: 27942195)
J Antimicrob Chemother. 1991 Jun;27(6):863-4. (PMID: 1938695)
Comp Immunol Microbiol Infect Dis. 2018 Oct;60:35-41. (PMID: 30396428)
J Mol Microbiol Biotechnol. 2001 Apr;3(2):255-64. (PMID: 11321581)
Pak J Med Sci. 2013 May;29(3):851-5. (PMID: 24353642)
Front Microbiol. 2011 Jul 13;2:150. (PMID: 21808635)
FEMS Microbiol Lett. 2005 Feb 1;243(1):271-8. (PMID: 15668029)
Am J Clin Pathol. 1966 Apr;45(4):493-6. (PMID: 5325707)
J Infect Public Health. 2013 Jun;6(3):179-85. (PMID: 23668462)
Signal Transduct Target Ther. 2022 Jun 25;7(1):199. (PMID: 35752612)
Antimicrob Agents Chemother. 2008 Jan;52(1):333-6. (PMID: 17967915)
J Clin Microbiol. 2004 May;42(5):2074-9. (PMID: 15131172)
Front Microbiol. 2011 Apr 05;2:65. (PMID: 21747788)
Front Cell Infect Microbiol. 2021 Nov 23;11:761417. (PMID: 34888259)
APMIS. 2013 Dec;121(12):1198-206. (PMID: 23594212)
BMC Vet Res. 2021 Mar 31;17(1):136. (PMID: 33789637)
Fitoterapia. 2012 Apr;83(3):508-12. (PMID: 22245085)
Phytomedicine. 2013 Jun 15;20(8-9):710-3. (PMID: 23537749)
J Antimicrob Chemother. 2007 Nov;60(5):1010-7. (PMID: 17906321)
Front Microbiol. 2015 Apr 28;6:377. (PMID: 25972857)
Cell Mol Biol (Noisy-le-grand). 2016 Jan 22;62(1):56-62. (PMID: 26828988)
Iran J Microbiol. 2016 Aug;8(4):249-256. (PMID: 28210464)
Microorganisms. 2016 Feb 16;4(1):. (PMID: 27681908)
Antimicrob Agents Chemother. 2019 Sep 23;63(10):. (PMID: 31383658)
J Bacteriol. 1993 Nov;175(22):7363-72. (PMID: 8226684)
J Bacteriol. 2000 Sep;182(18):5251-5. (PMID: 10960112)
BMC Microbiol. 2009 Nov 29;9:244. (PMID: 19943966)
Vet World. 2021 Aug;14(8):2155-2159. (PMID: 34566334)
Animals (Basel). 2021 Jun 22;11(7):. (PMID: 34206268)
Front Cell Infect Microbiol. 2017 Feb 15;7:39. (PMID: 28261568)
In Vivo. 2011 Mar-Apr;25(2):171-8. (PMID: 21471531)
Antimicrob Agents Chemother. 2005 May;49(5):1782-6. (PMID: 15855496)
Am J Crit Care. 2010 Nov;19(6):e81-7. (PMID: 21041189)
Nat Rev Drug Discov. 2007 Jan;6(1):56-65. (PMID: 17159924)
BMC Infect Dis. 2009 Aug 10;9:123. (PMID: 19664245)
Front Microbiol. 2015 Jul 08;6:660. (PMID: 26217310)
Front Plant Sci. 2017 Dec 22;8:2200. (PMID: 29312425)
فهرسة مساهمة: Keywords: MexB and MexA genes; Pseudomonas aeruginosa; antimicrobial activity; cinnamon oil; efflux pumps
تواريخ الأحداث: Date Created: 20231027 Latest Revision: 20231029
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10604284
DOI: 10.3390/antibiotics12101514
PMID: 37887215
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6382
DOI:10.3390/antibiotics12101514