دورية أكاديمية
أعرض في EDS

Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test.

التفاصيل البيبلوغرافية
العنوان: Diagnostic Potential for the Detection of Canine Visceral Leishmaniasis of an ELISA Assay Based on the Q5 Recombinant Protein: A Large-Scale and Comparative Evaluation Using Canine Sera with a Positive Diagnosis from the Dual-Path-Platform (DPP) Test.
المؤلفون: de Araújo Paz LF; Postgraduate Program in Health Sciences, University of Pernambuco, Recife 50100-010, Brazil., da Silva A; Aggeu Magalhães Institute-Fiocruz, Recife 50740-465, Brazil., da Silva HRF; Aggeu Magalhães Institute-Fiocruz, Recife 50740-465, Brazil., Cavalcanti MP; Aggeu Magalhães Institute-Fiocruz, Recife 50740-465, Brazil., de Lima VMF; Department of Clinic, Surgery and Animal Reproduction, College of Veterinary Medicine, São Paulo State University, Aracatuba 16050-680, Brazil., da Cunha Beltrão MRO; Postgraduate Program in Health Sciences, University of Pernambuco, Recife 50100-010, Brazil., Silva MBA; Postgraduate Program in Health Sciences, University of Pernambuco, Recife 50100-010, Brazil., de Melo Neto OP; Aggeu Magalhães Institute-Fiocruz, Recife 50740-465, Brazil., Medeiros ZM; Postgraduate Program in Health Sciences, University of Pernambuco, Recife 50100-010, Brazil.; Aggeu Magalhães Institute-Fiocruz, Recife 50740-465, Brazil., Santos WJTD; Aggeu Magalhães Institute-Fiocruz, Recife 50740-465, Brazil.
المصدر: Veterinary sciences [Vet Sci] 2023 Oct 07; Vol. 10 (10). Date of Electronic Publication: 2023 Oct 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101680127 Publication Model: Electronic Cited Medium: Internet ISSN: 2306-7381 (Electronic) Linking ISSN: 23067381 NLM ISO Abbreviation: Vet Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI, [2014]-
مستخلص: Dogs are considered the major domestic reservoir for human visceral leishmaniasis, a serious disease caused by the Leishmania infantum parasite. Diagnosis of canine visceral leishmaniasis (CVL) is critical for disease control, with several methods currently available. Among the serological tests, the DPP rapid test and the EIE-LVC, more commonly used in Brazil, are associated with variable sensitivity and specificity. Research with novel recombinant proteins such as the ELISA with the recombinant chimeric protein Q5 may therefore improve the CVL diagnosis. This study aimed to evaluate the true diagnostic potential of Q5 in an ELISA assay using a large number of CVL-suspected sera (406) with a previous positive diagnosis based on the rapid DPP test. Sera from the DPP-positive dogs, also assessed with the EIE-LVC test, were compared with sera from healthy dogs (n = 46) and used for ELISA tests using the recombinant Q5. The resulting data as well as the correlation with the clinical signs and the environmental characteristics of the animals were analyzed using Medal and GraphPad Prism 8.0. Overall, similar levels of lower sensitivity (67-68%) were seen for both the commercial EIE-LVC test and the Q5 ELISA when all assessed sera were considered, but a much greater sensitivity (92%) was seen for those samples from symptomatic dogs only. In contrast, many negative results were observed for the DPP-positive sera from asymptomatic dogs or those with no clinical information available. A selection of those sera were tested yet again in new ELISA assays using a second batch of the recombinant Q5, purified under milder denaturing conditions, as well as using another recombinant protein (Lci13). The results reveal a higher-than-expected incidence of likely false-positive results for DPP, reinforcing the need for other recombinant proteins, such as the chimeric Q5, to be investigated as possible alternatives to the currently used CVL diagnostic methods.
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معلومات مُعتمدة: APQ-0815- 2.13/17 Fundação de Amparo à Ciência e Tecnologia de Pernambuco
فهرسة مساهمة: Keywords: DPP test; Leishmania infantum; canine visceral leishmaniasis
تواريخ الأحداث: Date Created: 20231027 Latest Revision: 20231029
رمز التحديث: 20231029
مُعرف محوري في PubMed: PMC10610690
DOI: 10.3390/vetsci10100608
PMID: 37888560
قاعدة البيانات: MEDLINE
الوصف
تدمد:2306-7381
DOI:10.3390/vetsci10100608