دورية أكاديمية
Small molecule-mediated reprogramming of Xenopus blastula stem cells to a neural crest state.
| العنوان: | Small molecule-mediated reprogramming of Xenopus blastula stem cells to a neural crest state. |
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| المؤلفون: | Huber PB; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, IL 60208, USA., LaBonne C; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA; NSF-Simons Center for Quantitative Biology, Northwestern University, Evanston, IL 60208, USA. Electronic address: clabonne@northwestern.edu. |
| المصدر: | Developmental biology [Dev Biol] 2024 Jan; Vol. 505, pp. 34-41. Date of Electronic Publication: 2023 Oct 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372762 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-564X (Electronic) Linking ISSN: 00121606 NLM ISO Abbreviation: Dev Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: San Diego, CA : Elsevier Original Publication: New York. |
| مواضيع طبية MeSH: | Neural Crest*/metabolism , Pluripotent Stem Cells*, Animals ; Xenopus laevis/genetics ; Blastula/metabolism ; Cell Differentiation |
| مستخلص: | Neural crest cells are a stem cell population unique to vertebrates that give rise to a diverse array of derivatives, including much of the peripheral nervous system, pigment cells, cartilage, mesenchyme, and bone. Acquisition of these cells drove the evolution of vertebrates and defects in their development underlies a broad set of neurocristopathies. Moreover, studies of neural crest can inform differentiation protocols for pluripotent stem cells and regenerative medicine applications. Xenopus embryos are an important system for studies of the neural crest and have provided numerous insights into the signals and transcription factors that control the formation and later lineage diversification of these stem cells. Pluripotent animal pole explants are a particularly powerful tool in this system as they can be cultured in simple salt solution and instructed to give rise to any cell type including the neural crest. Here we report a protocol for small molecule-mediated induction of the neural crest state from blastula stem cells and validate it using transcriptome analysis and grafting experiments. This is an powerful new tool for generating this important cell type that will facilitate future studies of neural crest development and mutations and variants linked to neurocristopathies. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Acknowledgements We thank members of the LaBonne laboratory for helpful discussions. This work was supported by grants from the Simons Foundation (597491-RWC) the National Science Foundation (1764421) and the NIH (R01GM116538). (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: BMP; Neural crest; Stem cell; Wnt; Xenopus |
| تواريخ الأحداث: | Date Created: 20231027 Date Completed: 20231216 Latest Revision: 20231216 |
| رمز التحديث: | 20231217 |
| DOI: | 10.1016/j.ydbio.2023.10.004 |
| PMID: | 37890713 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-564X |
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| DOI: | 10.1016/j.ydbio.2023.10.004 |