دورية أكاديمية
High-Resolution Electron Diffraction of Hydrated Protein Crystals at Room Temperature.
العنوان: | High-Resolution Electron Diffraction of Hydrated Protein Crystals at Room Temperature. |
---|---|
المؤلفون: | Plana-Ruiz S; NanoMegas SRPL, Rue Emile Claus 49, Brussels 1050, Belgium.; Servei de Recursos Científics i Tècnics, Universitat Rovira i Virgili, Tarragona 43007, Catalonia, Spain., Gómez-Pérez A; NanoMegas SRPL, Rue Emile Claus 49, Brussels 1050, Belgium., Budayova-Spano M; Université Grenoble Alpes, CEA, CNRS, IBS, F-38000 Grenoble, France., Foley DL; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States., Portillo-Serra J; NanoMegas SRPL, Rue Emile Claus 49, Brussels 1050, Belgium., Rauch E; SIMAP, Grenoble INP, Université Grenoble Alpes, CNRS, F-38000 Grenoble, France., Grivas E; NanoMegas SRPL, Rue Emile Claus 49, Brussels 1050, Belgium., Housset D; Université Grenoble Alpes, CEA, CNRS, IBS, F-38000 Grenoble, France., Das PP; NanoMegas SRPL, Rue Emile Claus 49, Brussels 1050, Belgium., Taheri ML; Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States., Nicolopoulos S; NanoMegas SRPL, Rue Emile Claus 49, Brussels 1050, Belgium., Ling WL; Université Grenoble Alpes, CEA, CNRS, IBS, F-38000 Grenoble, France. |
المصدر: | ACS nano [ACS Nano] 2023 Dec 26; Vol. 17 (24), pp. 24802-24813. Date of Electronic Publication: 2023 Oct 27. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101313589 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1936-086X (Electronic) Linking ISSN: 19360851 NLM ISO Abbreviation: ACS Nano Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington D.C. : American Chemical Society |
مواضيع طبية MeSH: | Electrons* , Proteins*/chemistry, Temperature ; Crystallography, X-Ray ; X-Ray Diffraction |
مستخلص: | Structural characterization is crucial to understanding protein function. Compared with X-ray diffraction methods, electron crystallography can be performed on nanometer-sized crystals and can provide additional information from the resulting Coulomb potential map. Whereas electron crystallography has successfully resolved three-dimensional structures of vitrified protein crystals, its widespread use as a structural biology tool has been limited. One main reason is the fragility of such crystals. Protein crystals can be easily damaged by mechanical stress, change in temperature, or buffer conditions as well as by electron irradiation. This work demonstrates a methodology to preserve these nanocrystals in their natural environment at room temperature for electron diffraction experiments as an alternative to existing cryogenic techniques. Lysozyme crystals in their crystallization solution are hermetically sealed via graphene-coated grids, and their radiation damage is minimized by employing a low-dose data collection strategy in combination with a hybrid-pixel direct electron detector. Diffraction patterns with reflections of up to 3 Å are obtained and successfully indexed using a template-matching algorithm. These results demonstrate the feasibility of in situ protein electron diffraction. The method described will also be applicable to structural studies of hydrated nanocrystals important in many research and technological developments. |
References: | Proc Natl Acad Sci U S A. 2020 Feb 25;117(8):4142-4151. (PMID: 32047034) Q Rev Biophys. 1995 May;28(2):171-93. (PMID: 7568675) Q Rev Biophys. 1988 May;21(2):129-228. (PMID: 3043536) J Vis Exp. 2018 May 17;(135):. (PMID: 29863683) Acta Crystallogr D Struct Biol. 2021 Jan 1;77(Pt 1):75-85. (PMID: 33404527) ACS Cent Sci. 2019 Aug 28;5(8):1315-1329. (PMID: 31482114) BMC Bioinformatics. 2007 Aug 06;8:288. (PMID: 17683598) Science. 2009 Jan 30;323(5914):610-3. (PMID: 19179524) J Struct Biol. 2005 Oct;152(1):36-51. (PMID: 16182563) Sci Rep. 2016 Mar 01;6:22475. (PMID: 26926993) Nature. 1987 Feb 12-18;325(6105):643-6. (PMID: 3808065) Nanoscale. 2019 Jan 31;11(5):2299-2305. (PMID: 30662983) Small Methods. 2021 Jun;5(6):e2001287. (PMID: 34927906) J Mol Biol. 1994 Dec 9;244(4):436-47. (PMID: 7990132) Science. 2012 Apr 6;336(6077):61-4. (PMID: 22491849) J Appl Crystallogr. 2020 May 05;53(Pt 3):686-698. (PMID: 32684884) ACS Nano. 2018 Aug 28;12(8):8572-8578. (PMID: 30019893) Microsc Microanal. 2010 Oct;16(5):622-9. (PMID: 20804635) Nano Lett. 2017 Jan 11;17(1):414-420. (PMID: 28026186) Micron. 2023 Jun;169:103444. (PMID: 36965270) Acta Crystallogr D Struct Biol. 2017 Jun 01;73(Pt 6):503-508. (PMID: 28580912) Front Chem. 2022 Dec 20;10:1058620. (PMID: 36605121) Adv Mater. 2020 Jun;32(25):e2001582. (PMID: 32419161) Nat Commun. 2020 Feb 21;11(1):996. (PMID: 32081905) Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):339-47. (PMID: 17327671) Proc Natl Acad Sci U S A. 2020 Jan 14;117(2):1009-1014. (PMID: 31879346) J Appl Crystallogr. 2013 Oct 1;46(Pt 5):1501-1507. (PMID: 24068843) ACS Nano. 2022 Nov 22;16(11):18526-18537. (PMID: 36256532) Front Chem. 2022 Aug 30;10:889203. (PMID: 36110139) Curr Opin Struct Biol. 2018 Jun;50:109-116. (PMID: 29455056) Nat Commun. 2020 Mar 9;11(1):1271. (PMID: 32152274) Nat Methods. 2019 May;16(5):369-379. (PMID: 31040436) Acta Crystallogr B Struct Sci Cryst Eng Mater. 2019 Aug 1;75(Pt 4):463-474. (PMID: 32830704) Biophys Rep. 2017;3(1):1-7. (PMID: 28781996) J Appl Crystallogr. 2018 Aug 09;51(Pt 5):1262-1273. (PMID: 30279637) J Appl Crystallogr. 2014 May 29;47(Pt 3):1118-1131. (PMID: 24904246) Chem Commun (Camb). 2015 Nov 25;51(91):16393-6. (PMID: 26412024) Ultramicroscopy. 2018 Apr;187:113-125. (PMID: 29428430) Nano Lett. 2018 Dec 12;18(12):7435-7440. (PMID: 30431282) Nat Methods. 2014 Jun;11(6):649-52. (PMID: 24747813) Science. 1972 Jul 21;177(4045):268-70. (PMID: 5041024) Struct Dyn. 2020 Feb 13;7(1):014304. (PMID: 32071929) Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834) Front Mol Biosci. 2021 May 19;8:624264. (PMID: 34095217) |
فهرسة مساهمة: | Keywords: electron diffraction; graphene liquid cell; liquid phase electron microscopy; lysozyme; protein electron crystallography |
المشرفين على المادة: | 0 (Proteins) |
تواريخ الأحداث: | Date Created: 20231027 Date Completed: 20231227 Latest Revision: 20240829 |
رمز التحديث: | 20240830 |
مُعرف محوري في PubMed: | PMC10753879 |
DOI: | 10.1021/acsnano.3c05378 |
PMID: | 37890869 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1936-086X |
---|---|
DOI: | 10.1021/acsnano.3c05378 |