دورية أكاديمية
Mineral Solubilizing Rhizobacterial Strains Mediated Biostimulation of Rhodes Grass Seedlings.
العنوان: | Mineral Solubilizing Rhizobacterial Strains Mediated Biostimulation of Rhodes Grass Seedlings. |
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المؤلفون: | Javaid S; Institute of Molecular Biology and Biotechnology, The University of Lahore Main Campus, Lahore 54000, Pakistan., Mushtaq S; Institute of Molecular Biology and Biotechnology, The University of Lahore Main Campus, Lahore 54000, Pakistan., Mumtaz MZ; Institute of Molecular Biology and Biotechnology, The University of Lahore Main Campus, Lahore 54000, Pakistan., Rasool G; Institute of Molecular Biology and Biotechnology, The University of Lahore Main Campus, Lahore 54000, Pakistan., Naqqash T; Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan 60800, Pakistan., Afzal M; Institute of Molecular Biology and Biotechnology, The University of Lahore Main Campus, Lahore 54000, Pakistan., Mushtaq U; Institute of Molecular Biology and Biotechnology, The University of Lahore Main Campus, Lahore 54000, Pakistan., Ali HM; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia., Akhtar MF; Department of Soil Science, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan., Abbas G; Centre for Climate Research and Development, COMSATS University Islamabad, Islamabad 45550, Pakistan., Li L; College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.; State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China. |
المصدر: | Microorganisms [Microorganisms] 2023 Oct 12; Vol. 11 (10). Date of Electronic Publication: 2023 Oct 12. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, [2013]- |
مستخلص: | Minerals play a dynamic role in plant growth and development. However, most of these mineral nutrients are unavailable to plants due to their presence in fixed forms, which causes significant losses in crop production. An effective strategy to overcome this challenge is using mineral solubilizing bacteria, which can convert insoluble forms of minerals into soluble ones that plants can quickly assimilate, thus enhancing their availability in nutrient-depleted soils. The main objective of the present study was to isolate and characterize mineral solubilizing rhizobacteria and to assess their plant growth-promoting potential for Rhodes grass. Twenty-five rhizobacterial strains were isolated on a nutrient agar medium. They were characterized for solubilization of insoluble minerals (phosphate, potassium, zinc, and manganese), indole acetic acid production, enzymatic activities, and various morphological traits. The selected strains were also evaluated for their potential to promote the growth of Rhodes grass seedlings. Among tested strains, eight strains demonstrated strong qualitative and quantitative solubilization of insoluble phosphate. Strain MS2 reported the highest phosphate solubilization index, phosphate solubilization efficiency, available phosphorus concentration, and reduction in medium pH. Among tested strains, 75% were positive for zinc and manganese solubilization, and 37.5% were positive for potassium solubilization. Strain MS2 demonstrated the highest quantitative manganese solubilization, while strains MS7 and SM4 reported the highest solubilization of zinc and potassium through acidifying their respective media. The strain SM4 demonstrated the most increased IAA production in the presence and absence of L-tryptophan. The majority of strains were positive for various enzymes, including urease, catalase protease, and amylase activities. However, these strains were negative for coagulase activity except strains SM7 and MS7. Based on 16S rRNA gene sequencing, six strains, namely, SM2, SM4, SM5, MS1, MS2, and MS4, were identified as Bacillus cereus , while strains SM7 and MS7 were identified as Staphylococcus saprophyticus and Staphylococcus haemolyticus . These strains significantly improved growth attributes of Rhodes grass, such as root length, shoot length, and root and shoot fresh and dry biomasses compared to the uninoculated control group. The present study highlights the significance of mineral solubilizing and enzyme-producing rhizobacterial strains as potential bioinoculants to enhance Rhodes grass growth under mineral-deficient conditions sustainably. |
References: | Saudi J Biol Sci. 2019 Nov;26(7):1400-1410. (PMID: 31762601) Molecules. 2021 Mar 12;26(6):. (PMID: 33809305) Front Mol Biosci. 2019 Nov 05;6:115. (PMID: 31750314) Front Microbiol. 2015 Jul 22;6:745. (PMID: 26257721) Res Microbiol. 2016 Jul-Aug;167(6):510-20. (PMID: 27117242) Saudi J Biol Sci. 2021 Sep;28(9):5317-5324. (PMID: 34466110) World J Microbiol Biotechnol. 2021 Feb 5;37(3):39. (PMID: 33544268) Front Plant Sci. 2017 Feb 07;8:141. (PMID: 28224000) Arch Microbiol. 2021 Sep;203(7):3869-3882. (PMID: 34013419) FEMS Microbiol Rev. 2007 Jul;31(4):425-48. (PMID: 17509086) Microb Ecol. 2023 Jul;86(1):431-445. (PMID: 35867140) Front Microbiol. 2021 Jun 23;12:572212. (PMID: 34248859) Environ Sci Pollut Res Int. 2022 Jun;29(30):45089-45106. (PMID: 35474421) Folia Microbiol (Praha). 2021 Feb;66(1):115-125. (PMID: 33099750) J Plant Physiol. 2021 Dec;267:153544. (PMID: 34700019) Front Plant Sci. 2020 Feb 04;11:40. (PMID: 32117379) BMC Microbiol. 2009 Aug 22;9:174. (PMID: 19698133) J Genet Eng Biotechnol. 2017 Jun;15(1):169-178. (PMID: 30647653) Cells. 2021 Jun 19;10(6):. (PMID: 34205352) World J Microbiol Biotechnol. 2012 Apr;28(4):1327-50. (PMID: 22805914) Environ Sci Pollut Res Int. 2021 Aug;28(30):40249-40263. (PMID: 33011949) Int J Syst Evol Microbiol. 2019 Nov;69(11):3609-3615. (PMID: 31436526) World J Microbiol Biotechnol. 2017 Oct 6;33(11):197. (PMID: 28986676) 3 Biotech. 2020 Feb;10(2):36. (PMID: 31988830) Bioresour Technol. 2021 Oct;337:125433. (PMID: 34171708) Chemosphere. 2021 Jan;262:127803. (PMID: 32755694) Curr Microbiol. 2008 Feb;56(2):140-4. (PMID: 18026795) Bot Stud. 2017 Aug 16;58(1):35. (PMID: 28815474) Front Microbiol. 2017 Jun 02;8:971. (PMID: 28626450) Microbiol Res. 2017 Sep;202:51-60. (PMID: 28647123) Sci Rep. 2020 Sep 23;10(1):15536. (PMID: 32968101) PLoS One. 2018 Sep 21;13(9):e0204408. (PMID: 30240432) Curr Protoc Mol Biol. 2001 Nov;Chapter 2:Unit 2.4. (PMID: 18265184) FEMS Microbiol Lett. 2002 Jul 16;213(1):1-6. (PMID: 12127480) Heliyon. 2022 Dec 09;8(12):e12224. (PMID: 36582684) Ecotoxicol Environ Saf. 2019 May 15;172:159-166. (PMID: 30708227) Molecules. 2021 Mar 26;26(7):. (PMID: 33810565) J Bacteriol. 2004 Feb;186(3):595-600. (PMID: 14729683) Burns. 2008 Mar;34(2):205-11. (PMID: 18037568) Plants (Basel). 2020 Nov 17;9(11):. (PMID: 33213067) Bioresour Technol. 2018 Jan;247:190-199. (PMID: 28950126) Microbiologyopen. 2018 Dec;7(6):e00617. (PMID: 29653035) Saudi J Biol Sci. 2019 Jul;26(5):1061-1067. (PMID: 31303841) Metabolites. 2020 Oct 31;10(11):. (PMID: 33142690) Front Plant Sci. 2021 Nov 02;12:719504. (PMID: 34795682) Genes (Basel). 2021 Aug 10;12(8):. (PMID: 34440407) |
فهرسة مساهمة: | Keywords: Bacillus; Chloris gayana; Staphylococcus; enzymatic activities; mineral solubilization; partial gene sequencing |
تواريخ الأحداث: | Date Created: 20231028 Latest Revision: 20231030 |
رمز التحديث: | 20231030 |
مُعرف محوري في PubMed: | PMC10609362 |
DOI: | 10.3390/microorganisms11102543 |
PMID: | 37894201 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2076-2607 |
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DOI: | 10.3390/microorganisms11102543 |