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Diverse Regulatory Manners and Potential Roles of lncRNAs in the Developmental Process of Asian Honey Bee ( Apis cerana ) Larval Guts.

التفاصيل البيبلوغرافية
العنوان: Diverse Regulatory Manners and Potential Roles of lncRNAs in the Developmental Process of Asian Honey Bee ( Apis cerana ) Larval Guts.
المؤلفون: Fan X; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Gao X; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Zang H; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Guo S; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Jing X; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Zhang Y; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Liu X; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Zou P; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Chen M; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Huang Z; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China., Chen D; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China.; Apitherapy Research Institute of Fujian Province, Fuzhou 350002, China., Guo R; College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China.; Apitherapy Research Institute of Fujian Province, Fuzhou 350002, China.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Oct 20; Vol. 24 (20). Date of Electronic Publication: 2023 Oct 20.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: RNA, Long Noncoding*/genetics , RNA, Long Noncoding*/metabolism , MicroRNAs*/genetics, Bees/genetics ; Animals ; Larva/genetics ; Larva/metabolism ; Hedgehog Proteins/genetics ; Reproducibility of Results ; RNA, Messenger/genetics ; Gene Regulatory Networks
مستخلص: Long non-coding RNAs (lncRNAs) are crucial modulators in a variety of biological processes, such as gene expression, development, and immune defense. However, little is known about the function of lncRNAs in the development of Asian honey bee ( Apis cerana ) larval guts. Here, on the basis of our previously obtained deep-sequencing data from the 4-, 5-, and 6-day-old larval guts of A. cerana workers (Ac4, Ac5, and Ac6 groups), an in-depth transcriptome-wide investigation was conducted to decipher the expression pattern, regulatory manners, and potential roles of lncRNAs during the developmental process of A. cerana worker larval guts, followed by the verification of the relative expression of differentially expressed lncRNAs (DElncRNAs) and the targeting relationships within a competing endogenous RNA (ceRNA) axis. In the Ac4 vs. Ac5 and Ac5 vs. Ac6 comparison groups, 527 and 498 DElncRNAs were identified, respectively, which is suggestive of the dynamic expression of lncRNAs during the developmental process of larval guts. A cis -acting analysis showed that 330 and 393 neighboring genes of the aforementioned DElncRNAs were respectively involved in 29 and 32 functional terms, such as cellular processes and metabolic processes; these neighboring genes were also respectively engaged in 246 and 246 pathways such as the Hedgehog signaling pathway and the Wnt signaling pathway. Additionally, it was found that 79 and 76 DElncRNAs as potential antisense lncRNAs may, respectively, interact with 72 and 60 sense-strand mRNAs. An investigation of competing endogenous RNA (ceRNA) networks suggested that 75 (155) DElncRNAs in the Ac4 vs. Ac5 (Ac5 vs. Ac6) comparison group could target 7 (5) DEmiRNAs and further bind to 334 (248) DEmRNAs, which can be annotated to 33 (29) functional terms and 186 (210) pathways, including 12 (16) cellular- and humoral-immune pathways (lysosome pathway, necroptosis, MAPK signaling pathway, etc.) and 11 (10) development-associated signaling pathways (Wnt, Hippo, AMPK, etc.). The RT-qPCR detection of five randomly selected DElncRNAs confirmed the reliability of the used sequencing data. Moreover, the results of a dual-luciferase reporter assay were indicative of the binding relationship between MSTRG.11294.1 and miR-6001-y and between miR-6001-y and ncbi_107992440. These results demonstrate that DElncRNAs are likely to modulate the developmental process of larval guts via the regulation of the source genes' transcription, interaction with mRNAs, and ceRNA networks. Our findings not only yield new insights into the developmental mechanism underlying A. cerana larval guts, but also provide a candidate ceRNA axis for further functional dissection.
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معلومات مُعتمدة: 31702190 National Natural Science Foundation of China; CARS-44-KXJ7 Earmarked Fund for China Agriculture Research System; Rui Guo Master Supervisor Team Fund of Fujian Agriculture and Forestry University; KFb22060XA the Scientific and Technical Innovation Fund of Fujian Agriculture and Forestry University; Rui Guo Scientific Research Project of College of Animal Sciences (College of Bee Science) of Fujian Agri-culture and Forestry University; S202310389082,X202310389087, X202310389075 Undergraduate Innovation and Entrepreneurship Training Program of Fujian province
فهرسة مساهمة: Keywords: Apis cerana cerana; development; gut; honey bee; lncRNA; non-coding RNA; regulation
المشرفين على المادة: 0 (RNA, Long Noncoding)
0 (Hedgehog Proteins)
0 (RNA, Messenger)
0 (MicroRNAs)
تواريخ الأحداث: Date Created: 20231028 Date Completed: 20231030 Latest Revision: 20231030
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10607868
DOI: 10.3390/ijms242015399
PMID: 37895079
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms242015399