دورية أكاديمية
Enhanced glucose homeostasis via Clostridium symbiosum -mediated glucagon-like peptide 1 inhibition of hepatic gluconeogenesis in mid-intestinal bypass surgery.
| العنوان: | Enhanced glucose homeostasis via Clostridium symbiosum -mediated glucagon-like peptide 1 inhibition of hepatic gluconeogenesis in mid-intestinal bypass surgery. |
|---|---|
| المؤلفون: | Luo X; Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China., Tao F; Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China., Tan C; Department of Women's Health, Jiangxi Maternal and Child Health Hospital, Nanchang 330000, Jiangxi Province, China., Xu CY; Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China., Zheng ZH; Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China., Pang Q; Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China., He XA; Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China., Cao JQ; Department of General Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China., Duan JY; Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China. duanjy2022@outlook.com. |
| المصدر: | World journal of gastroenterology [World J Gastroenterol] 2023 Oct 21; Vol. 29 (39), pp. 5471-5482. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Baishideng Publishing Group Country of Publication: United States NLM ID: 100883448 Publication Model: Print Cited Medium: Internet ISSN: 2219-2840 (Electronic) Linking ISSN: 10079327 NLM ISO Abbreviation: World J Gastroenterol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2014- : Pleasanton, CA : Baishideng Publishing Group Original Publication: Beijing : WJG Press, c1998- |
| مواضيع طبية MeSH: | Clostridium symbiosum*/metabolism , Diabetes Mellitus, Experimental*/surgery , Gastric Bypass*, Rats ; Animals ; Gluconeogenesis/physiology ; Glucagon-Like Peptide 1/metabolism ; Jejunoileal Bypass ; Rats, Sprague-Dawley ; Glucose/metabolism ; Homeostasis ; Blood Glucose/metabolism |
| مستخلص: | Background: The small intestine is known to play a crucial role in the development and remission of diabetes mellitus (DM). However, the exact mechanism by which mid-small intestinal bypass improves glucose metabolism in diabetic rats is not fully understood. Aim: To elucidate the mechanisms by which mid-small intestinal bypass improves glucose metabolism. Methods: Streptozotocin (STZ) was used to induce DM in Sprague-Dawley (SD) rats at a dose of 60 mg/kg. The rats were then randomly divided into two groups: The mid-small intestine bypass (MSIB) group and the sham group (underwent switch laparotomy). Following a 6-wk recovery period post-surgery, the rats underwent various assessments, including metabolic parameter testing, analysis of liver glycogen levels, measurement of key gluconeogenic enzyme activity, characterization of the gut microbiota composition, evaluation of hormone levels, determination of bile acid concentrations, and assessment of the expression of the intestinal receptors Takeda G protein-coupled receptor 5 and farnesoid X receptor. Results: The MSIB group of rats demonstrated improved glucose metabolism and lipid metabolism, along with increased hepatic glycogen content. Furthermore, there was a decrease in the expression of the key gluconeogenic enzymes phosphoenolpyruvate carboxykinase 1 and glucose-6-phosphatase. Importantly, the MSIB group exhibited a substantial increase in the abundances of intestinal Lactobacillus , Clostridium symbiosum , Ruminococcus gnavus , and Bilophila . Moreover, higher levels of secondary bile acids, such as intestinal lithocholic acid, were observed in this group. Remarkably, the changes in the gut microbiota showed a significant correlation with the expression of key gluconeogenic enzymes and glucagon-like peptide 1 (GLP-1) at 6 wk postoperatively, highlighting their potential role in glucose regulation. These findings highlight the beneficial effects of mid-small intestine bypass on glucose metabolism and the associated modulation of the gut microbiota. Conclusion: The findings of this study demonstrate that the introduction of postoperative intestinal Clostridium symbiosum in the mid-small intestine contributes to the enhancement of glucose metabolism in nonobese diabetic rats. This improvement is attributed to the increased inhibition of hepatic gluconeogenesis mediated by GLP-1, resulting in a favorable modulation of glucose homeostasis. Competing Interests: Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article. (©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.) |
| References: | Gastroenterology. 2010 Jun;138(7):2437-46, 2446.e1. (PMID: 20226188) Gastroenterology. 1990 Jan;98(1):111-6. (PMID: 2293569) Lancet Gastroenterol Hepatol. 2021 Mar;6(3):225-237. (PMID: 33581761) J Hepatol. 2014 Feb;60(2):377-83. (PMID: 24060855) Dtsch Arztebl Int. 2022 Feb 4;119(5):70-80. (PMID: 34819222) J Biol Chem. 2017 Jun 30;292(26):11055-11069. (PMID: 28478385) Nat Commun. 2022 Oct 27;13(1):6408. (PMID: 36302774) Nat Methods. 2013 Jan;10(1):57-9. (PMID: 23202435) Lancet. 2017 Dec 16;390(10113):2627-2642. (PMID: 29029897) EBioMedicine. 2019 Mar;41:73-84. (PMID: 30827929) Diabetes Care. 2019 Apr;42(4):520-528. (PMID: 30765429) Cell Metab. 2013 Feb 5;17(2):225-35. (PMID: 23395169) Cell Death Dis. 2017 Feb 9;8(2):e2609. (PMID: 28182013) Cell. 2022 Jul 7;185(14):2393-2395. (PMID: 35803241) Am J Physiol Endocrinol Metab. 2013 Dec 1;305(11):E1348-58. (PMID: 24085036) Gut. 2021 Feb;70(2):319-329. (PMID: 32409590) Diabetes. 2007 Jun;56(6):1671-9. (PMID: 17369525) Dig Liver Dis. 2014 Apr;46(4):302-12. (PMID: 24411485) Diabetes. 2021 Oct;70(10):2289-2298. (PMID: 34341005) Metabolism. 2000 Nov;49(11):1390-4. (PMID: 11092499) |
| فهرسة مساهمة: | Keywords: Bariatric surgery; Bile acid; Glucagon-like peptide-1; Gluconeogenesis; Glucose metablism; Gut micobiome |
| المشرفين على المادة: | 89750-14-1 (Glucagon-Like Peptide 1) IY9XDZ35W2 (Glucose) 0 (Blood Glucose) |
| تواريخ الأحداث: | Date Created: 20231030 Date Completed: 20231031 Latest Revision: 20231031 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10600812 |
| DOI: | 10.3748/wjg.v29.i39.5471 |
| PMID: | 37900993 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2219-2840 |
|---|---|
| DOI: | 10.3748/wjg.v29.i39.5471 |