التفاصيل البيبلوغرافية
| العنوان: |
Addressing the dNTP bottleneck restricting prime editing activity. |
| المؤلفون: |
Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Joynt AT, Kelly K, Guo D, Chen Z, Lee JM, Schiffer CA, Emerson CP, Lawson ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Oct 29. Date of Electronic Publication: 2023 Oct 29. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
Prime editing efficiency is modest in cells that are quiescent or slowly proliferating where intracellular dNTP levels are tightly regulated. MMLV-reverse transcriptase - the prime editor polymerase subunit - requires high intracellular dNTPs levels for efficient polymerization. We report that prime editing efficiency in primary cells and in vivo is increased by mutations that enhance the enzymatic properties of MMLV-reverse transcriptase and can be further complemented by targeting SAMHD1 for degradation. |
| معلومات مُعتمدة: |
U54 AI170856 United States AI NIAID NIH HHS |
| تواريخ الأحداث: |
Date Created: 20231031 Latest Revision: 20240217 |
| رمز التحديث: |
20240217 |
| مُعرف محوري في PubMed: |
PMC10614944 |
| DOI: |
10.1101/2023.10.21.563443 |
| PMID: |
37904991 |
| قاعدة البيانات: |
MEDLINE |
