دورية أكاديمية
أعرض في EDS

Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain.

التفاصيل البيبلوغرافية
العنوان: Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain.
المؤلفون: LaManna L; Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, 08854, USA., Chou CH; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, FL, 32611, USA., Lei H; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, FL, 32611, USA., Barton ER; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, FL, 32611, USA. erbarton@ufl.edu., Maliga P; Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, 08854, USA. maliga@waksman.rutgers.edu.; Department of Plant Biology, Rutgers University, New Brunswick, NJ, 08901, USA. maliga@waksman.rutgers.edu.
المصدر: Scientific reports [Sci Rep] 2023 Nov 02; Vol. 13 (1), pp. 18916. Date of Electronic Publication: 2023 Nov 02.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Immunoglobulin G*/genetics , Immunoglobulin G*/metabolism , Chloroplasts*/genetics , Chloroplasts*/metabolism, Mice ; Animals ; Nicotiana/genetics ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism
مستخلص: Proinsulin Like Growth Factor I (prolGF-I) and myostatin (Mstn) regulate muscle regeneration and mass when intravenously delivered. We tested if chloroplast bioencapsulated forms of these proteins may serve as a non-invasive means of drug delivery through the digestive system. We created tobacco (Nicotiana tabacum) plants carrying GFP-Fc1, proIGF-I-Fc1, and Mstn-Fc1 fusion genes, in which fusion with the immunoglobulin G Fc domain improved both protein stability and absorption in the small intestine. No transplastomic plants were obtained with the Mstn-Fc1 gene, suggesting that the protein is toxic to plant cells. proIGF-I-Fc1 protein levels were too low to enable in vivo testing. However, GFP-Fc1 accumulated at a high level, enabling evaluation of chloroplast-made Fc fusion proteins for oral delivery. Tobacco leaves were lyophilized for testing in a mouse system. We report that the orally administered GFP-Fc1 fusion protein (5.45 µg/g GFP-Fc1) has been taken up by the intestinal epithelium cells, evidenced by confocal microscopy. GFP-Fc1 subsequently entered the circulation where it was detected by ELISA. Data reported here confirm that chloroplast expression and oral administration of lyophilized leaves is a potential delivery system of therapeutic proteins fused with Fc1, with the advantage that the proteins may be stored at room temperature.
(© 2023. The Author(s).)
التعليقات: Update of: Res Sq. 2023 Jul 26;:. (PMID: 37546919)
References: J Immunol. 1989 Oct 15;143(8):2595-601. (PMID: 2507634)
Biomaterials. 2016 Feb;80:68-79. (PMID: 26706477)
Protein Cell. 2018 Jan;9(1):15-32. (PMID: 28421387)
Proc Natl Acad Sci U S A. 2000 May 23;97(11):5995-6000. (PMID: 10801973)
J Clin Invest. 2019 Sep 3;129(9):3492-3498. (PMID: 31478910)
Nat Plants. 2019 May;5(5):486-490. (PMID: 31036913)
Arch Neurol. 2011 Jan;68(1):37-44. (PMID: 20837825)
EMBO J. 2003 Feb 3;22(3):621-32. (PMID: 12554662)
Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):913-7. (PMID: 8381537)
Nature. 1997 May 1;387(6628):83-90. (PMID: 9139826)
Oncol Rep. 2014 Sep;32(3):893-900. (PMID: 25018100)
Plant Biotechnol J. 2015 Oct;13(8):1017-22. (PMID: 26333301)
Plant J. 2009 Feb;57(3):436-45. (PMID: 18939966)
Plant Mol Biol. 2018 Jul;97(4-5):357-370. (PMID: 29948657)
Plant Mol Biol. 2009 Jul;70(5):487-98. (PMID: 19387846)
Biomaterials. 2020 Mar;233:119591. (PMID: 31870566)
Plant Cell. 2003 Jan;15(1):195-205. (PMID: 12509531)
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W126-31. (PMID: 17439967)
Growth Factors. 2011 Oct;29(5):174-86. (PMID: 21864080)
Crit Rev Biotechnol. 2015 Jun;35(2):235-54. (PMID: 24156398)
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15842-6. (PMID: 14671324)
Nat Protoc. 2015 Jun;10(6):845-58. (PMID: 25950237)
Annu Rev Genet. 2016 Nov 23;50:595-618. (PMID: 27893966)
PLoS One. 2012;7(8):e42405. (PMID: 22879967)
Plant J. 2020 Sep;103(6):2318-2329. (PMID: 32497322)
J Biol Chem. 2007 Jan 19;282(3):1709-17. (PMID: 17135257)
Plant Cell. 1998 Oct;10(10):1713-22. (PMID: 9761797)
Nucleic Acids Res. 2003 Feb 15;31(4):1174-9. (PMID: 12582236)
Growth Horm IGF Res. 2014 Oct;24(5):157-63. (PMID: 25002025)
J Biol Chem. 2004 Feb 20;279(8):6213-6. (PMID: 14699147)
Biotechnology (N Y). 1995 Apr;13(4):362-5. (PMID: 9634777)
Endocrinology. 2013 Mar;154(3):1215-24. (PMID: 23407451)
Nucleic Acids Res. 1994 Sep 25;22(19):3819-24. (PMID: 7937099)
Plant Physiol. 2023 Apr 3;191(4):2229-2244. (PMID: 36510848)
Annu Rev Cell Dev Biol. 2004;20:61-86. (PMID: 15473835)
Nat Biotechnol. 2000 Mar;18(3):333-8. (PMID: 10700152)
BMC Biol. 2017 Mar 3;15(1):19. (PMID: 28257634)
Nat Commun. 2019 Nov 6;10(1):5031. (PMID: 31695028)
J Immunol. 2006 Jan 1;176(1):346-56. (PMID: 16365427)
Exp Clin Endocrinol Diabetes. 2015 Jun;123(6):371-5. (PMID: 25962403)
Appl Physiol Nutr Metab. 2006 Dec;31(6):791-7. (PMID: 17213901)
J Immunol. 2009 Jun 15;182(12):7663-71. (PMID: 19494290)
EMBO J. 2009 Jul 22;28(14):2042-52. (PMID: 19424177)
Growth Horm IGF Res. 2009 Feb;19(1):12-23. (PMID: 18571449)
Plant Physiol. 2009 Jul;150(3):1494-502. (PMID: 19411372)
PLoS One. 2012;7(9):e45588. (PMID: 23029120)
Elife. 2014 Sep 25;3:. (PMID: 25255214)
Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):415-20. (PMID: 21173259)
J Clin Endocrinol Metab. 2001 Apr;86(4):1496-503. (PMID: 11297574)
Nat Biotechnol. 2010 Feb;28(2):157-9. (PMID: 20081867)
BMC Biotechnol. 2016 Oct 19;16(1):72. (PMID: 27756365)
Kidney Int. 2006 Aug;70(3):410-2. (PMID: 16871252)
Plant Biotechnol J. 2018 Jul;16(7):1283-1294. (PMID: 29223138)
Front Plant Sci. 2021 Jul 09;12:671728. (PMID: 34305971)
Matrix Biol. 2007 Sep;26(7):508-23. (PMID: 17560775)
Plant J. 2004 Mar;37(6):906-13. (PMID: 14996222)
Science. 1990 Mar 16;247(4948):1328-30. (PMID: 2315700)
J Biol Chem. 2001 Mar 2;276(9):6591-604. (PMID: 11096108)
Blood. 2012 Mar 29;119(13):3024-30. (PMID: 22246033)
Drug Metab Dispos. 2007 Jan;35(1):86-94. (PMID: 17050651)
Nat Protoc. 2006;1(2):900-10. (PMID: 17406323)
Bioengineering (Basel). 2017 May 17;4(2):. (PMID: 28952523)
EMBO J. 1999 Jan 4;18(1):249-57. (PMID: 9878067)
Methods Mol Biol. 2014;1132:147-63. (PMID: 24599851)
Biochim Biophys Acta. 1989 May 10;1011(2-3):192-7. (PMID: 2469478)
Int Immunol. 2006 Dec;18(12):1759-69. (PMID: 17077181)
Endocr Rev. 1995 Feb;16(1):3-34. (PMID: 7758431)
Gene. 1982 Nov;20(1):91-102. (PMID: 7160620)
EMBO J. 2018 Feb 1;37(3):367-383. (PMID: 29330193)
Neurology. 1997 Dec;49(6):1621-30. (PMID: 9409357)
Trends Plant Sci. 2019 Oct;24(10):917-926. (PMID: 31300194)
BMC Biotechnol. 2009 Apr 03;9:33. (PMID: 19344517)
Tanpakushitsu Kakusan Koso. 1984 Nov;29(11):875-88. (PMID: 6395189)
Front Plant Sci. 2017 Feb 28;8:274. (PMID: 28293250)
Biotechnol Rep (Amst). 2019 Feb 08;21:e00312. (PMID: 30847284)
Arch Biochem Biophys. 2012 Oct 15;526(2):167-73. (PMID: 22634260)
Curr Opin Immunol. 2008 Aug;20(4):460-70. (PMID: 18656541)
J Immunol. 2002 Nov 1;169(9):5171-80. (PMID: 12391234)
معلومات مُعتمدة: R21 AR074271 United States AR NIAMS NIH HHS
المشرفين على المادة: 0 (Immunoglobulin G)
تواريخ الأحداث: Date Created: 20231103 Date Completed: 20231106 Latest Revision: 20240210
رمز التحديث: 20240210
مُعرف محوري في PubMed: PMC10622566
DOI: 10.1038/s41598-023-45698-9
PMID: 37919321
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-45698-9