دورية أكاديمية
Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain.
العنوان: | Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain. |
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المؤلفون: | LaManna L; Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, 08854, USA., Chou CH; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, FL, 32611, USA., Lei H; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, FL, 32611, USA., Barton ER; Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, FL, 32611, USA. erbarton@ufl.edu., Maliga P; Waksman Institute of Microbiology, Rutgers University, Piscataway, NJ, 08854, USA. maliga@waksman.rutgers.edu.; Department of Plant Biology, Rutgers University, New Brunswick, NJ, 08901, USA. maliga@waksman.rutgers.edu. |
المصدر: | Scientific reports [Sci Rep] 2023 Nov 02; Vol. 13 (1), pp. 18916. Date of Electronic Publication: 2023 Nov 02. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Immunoglobulin G*/genetics , Immunoglobulin G*/metabolism , Chloroplasts*/genetics , Chloroplasts*/metabolism, Mice ; Animals ; Nicotiana/genetics ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism |
مستخلص: | Proinsulin Like Growth Factor I (prolGF-I) and myostatin (Mstn) regulate muscle regeneration and mass when intravenously delivered. We tested if chloroplast bioencapsulated forms of these proteins may serve as a non-invasive means of drug delivery through the digestive system. We created tobacco (Nicotiana tabacum) plants carrying GFP-Fc1, proIGF-I-Fc1, and Mstn-Fc1 fusion genes, in which fusion with the immunoglobulin G Fc domain improved both protein stability and absorption in the small intestine. No transplastomic plants were obtained with the Mstn-Fc1 gene, suggesting that the protein is toxic to plant cells. proIGF-I-Fc1 protein levels were too low to enable in vivo testing. However, GFP-Fc1 accumulated at a high level, enabling evaluation of chloroplast-made Fc fusion proteins for oral delivery. Tobacco leaves were lyophilized for testing in a mouse system. We report that the orally administered GFP-Fc1 fusion protein (5.45 µg/g GFP-Fc1) has been taken up by the intestinal epithelium cells, evidenced by confocal microscopy. GFP-Fc1 subsequently entered the circulation where it was detected by ELISA. Data reported here confirm that chloroplast expression and oral administration of lyophilized leaves is a potential delivery system of therapeutic proteins fused with Fc1, with the advantage that the proteins may be stored at room temperature. (© 2023. The Author(s).) |
التعليقات: | Update of: Res Sq. 2023 Jul 26;:. (PMID: 37546919) |
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معلومات مُعتمدة: | R21 AR074271 United States AR NIAMS NIH HHS |
المشرفين على المادة: | 0 (Immunoglobulin G) |
تواريخ الأحداث: | Date Created: 20231103 Date Completed: 20231106 Latest Revision: 20240210 |
رمز التحديث: | 20240210 |
مُعرف محوري في PubMed: | PMC10622566 |
DOI: | 10.1038/s41598-023-45698-9 |
PMID: | 37919321 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/s41598-023-45698-9 |