تقرير
A single-domain intrabody targeting the follicle-stimulating hormone receptor impacts FSH-induced G protein-dependent signalling.
| العنوان: | A single-domain intrabody targeting the follicle-stimulating hormone receptor impacts FSH-induced G protein-dependent signalling. |
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| المؤلفون: | Raynaud P; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France., Jugnarain V; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France., Vaugrente O; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France., Vallet A; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France., Boulo T; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France., Gauthier C; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France., Inoue A; Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan., Sibille N; Centre de Biologie Structurale (CBS), CNRS, University Montpellier, Inserm, France., Gauthier C; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France., Jean-Alphonse F; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France.; Inria, Inria Saclay-Ile-de-France, Palaiseau, France., Reiter E; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France.; Inria, Inria Saclay-Ile-de-France, Palaiseau, France., Crépieux P; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France.; Inria, Inria Saclay-Ile-de-France, Palaiseau, France., Bruneau G; Physiologie de la Reproduction et des Comportements (PRC), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS), Institut Français du Cheval et de l'Equitation (IFCE), Université de Tours, Nouzilly, France. |
| المصدر: | FEBS letters [FEBS Lett] 2024 Jan; Vol. 598 (2), pp. 220-232. Date of Electronic Publication: 2023 Nov 03. |
| نوع المنشور: | Letter; Research Support, Non-U.S. Gov't; Comment |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3468 (Electronic) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd. Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies. |
| مواضيع طبية MeSH: | Receptors, FSH*/genetics , Receptors, FSH*/chemistry , Receptors, FSH*/metabolism , Follicle Stimulating Hormone*/chemistry , Follicle Stimulating Hormone*/metabolism , Follicle Stimulating Hormone*/pharmacology, GTP-Binding Proteins/metabolism ; Signal Transduction |
| مستخلص: | Intracellular variable fragments of heavy-chain antibody from camelids (intra-VHH) have been successfully used as chaperones to solve the 3D structure of active G protein-coupled receptors bound to their transducers. However, their effect on signalling has been poorly explored, although they may provide a better understanding of the relationships between receptor conformation and activity. Here, we isolated and characterized iPRC1, the first intra-VHH recognizing a member of the large glycoprotein hormone receptor family, the follicle-stimulating hormone receptor (FSHR). This intra-VHH recognizes the FSHR third intracellular loop and decreases cAMP production in response to FSH, without altering Gαs recruitment. Hence, iPRC1 behaves as an allosteric modulator and provides a new tool to complete structure/activity studies performed thus far on this receptor. (© 2023 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.) |
| التعليقات: | Comment on: Front Endocrinol (Lausanne). 2022 Nov 16;13:1048601. (PMID: 36465650) |
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| معلومات مُعتمدة: | Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement; ANR-10-LABX-53 LabEx MAbImprove; Région Centre Val de Loire RD2020 Biomédicaments SELMAT; INTACT Région Centre Val de Loire APR-IR; Centre National de la Recherche Scientifique (CNRS) |
| فهرسة مساهمة: | Keywords: FSH receptor; G protein-coupled receptors; cell signalling; intracellular VHH |
| المشرفين على المادة: | 0 (Receptors, FSH) 9002-68-0 (Follicle Stimulating Hormone) EC 3.6.1.- (GTP-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20231103 Date Completed: 20240130 Latest Revision: 20240206 |
| رمز التحديث: | 20240206 |
| DOI: | 10.1002/1873-3468.14765 |
| PMID: | 37923554 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3468 |
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| DOI: | 10.1002/1873-3468.14765 |