دورية أكاديمية
أعرض في EDS

Solution Structure of Poly(UG) RNA.

التفاصيل البيبلوغرافية
العنوان: Solution Structure of Poly(UG) RNA.
المؤلفون: Escobar CA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA., Petersen RJ; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA., Tonelli M; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA; National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, USA., Fan L; Basic Science Program, Frederick National Laboratory for Cancer Research, SAXS Core Facility of National Cancer Institute, Frederick, MD, USA., Henzler-Wildman KA; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA; National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, USA., Butcher SE; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA; National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: sebutcher@wisc.edu.
المصدر: Journal of molecular biology [J Mol Biol] 2023 Dec 15; Vol. 435 (24), pp. 168340. Date of Electronic Publication: 2023 Nov 02.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2985088R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1089-8638 (Electronic) Linking ISSN: 00222836 NLM ISO Abbreviation: J Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: 1959- : London : Academic Press
مواضيع طبية MeSH: RNA*/chemistry , Poly U*/chemistry , Poly G*/chemistry, Animals ; Caenorhabditis elegans/genetics ; Crystallography, X-Ray ; Ligands ; Models, Molecular ; Scattering, Small Angle ; X-Ray Diffraction ; Nucleic Acid Conformation
مستخلص: Poly(UG) or "pUG" RNAs are UG or GU dinucleotide repeat sequences which are highly abundant in eukaryotes. Post-transcriptional addition of pUGs to RNA 3' ends marks mRNAs as vectors for gene silencing in C. elegans. We previously determined the crystal structure of pUG RNA bound to the ligand N-methyl mesoporphyrin IX (NMM), but the structure of free pUG RNA is unknown. Here we report the solution structure of the free pUG RNA (GU) 12 , as determined by nuclear magnetic resonance spectroscopy and small and wide-angle x-ray scattering (NMR-SAXS-WAXS). The low complexity sequence and 4-fold symmetry of the structure result in overlapped NMR signals that complicate chemical shift assignment. We therefore utilized single site-specific deoxyribose modifications which did not perturb the structure and introduced well-resolved methylene signals that are easily identified in NMR spectra. The solution structure ensemble has a root mean squared deviation (RMSD) of 0.62 Å and is a compact, left-handed quadruplex with a Z-form backbone, or "pUG fold." Overall, the structure agrees with the crystal structure of (GU) 12 bound to NMM, indicating the pUG fold is unaltered by docking of the NMM ligand. The solution structure reveals conformational details that could not be resolved by x-ray crystallography, which explain how the pUG fold can form within longer RNAs.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023. Published by Elsevier Ltd.)
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معلومات مُعتمدة: 75N91019D00024 United States CA NCI NIH HHS; P41 GM136463 United States GM NIGMS NIH HHS; R24 GM141526 United States GM NIGMS NIH HHS; R35 GM118131 United States GM NIGMS NIH HHS; P41 GM111135 United States GM NIGMS NIH HHS; P41 GM103399 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: NMR; Quadruplex; RNA; SAXS; poly(UG)
المشرفين على المادة: 0 (Ligands)
63231-63-0 (RNA)
26680-26-2 (poly(U-G))
27416-86-0 (Poly U)
25191-14-4 (Poly G)
تواريخ الأحداث: Date Created: 20231104 Date Completed: 20240108 Latest Revision: 20240207
رمز التحديث: 20240207
مُعرف محوري في PubMed: PMC10841838
DOI: 10.1016/j.jmb.2023.168340
PMID: 37924862
قاعدة البيانات: MEDLINE
الوصف
تدمد:1089-8638
DOI:10.1016/j.jmb.2023.168340