دورية أكاديمية
أعرض في EDS

Detection of multidrug-resistant Campylobacter species from food-producing animals and humans in Nigeria: Public health implications and one health control measures.

التفاصيل البيبلوغرافية
العنوان: Detection of multidrug-resistant Campylobacter species from food-producing animals and humans in Nigeria: Public health implications and one health control measures.
المؤلفون: Njoga EO; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Nigeria, Nsukka 410001, Nigeria; Animal Health Antimicrobial Resistance Surveillance Sentinel Laboratory, Veterinary Teaching Hospital, University of Nigeria, Nsukka 410001, Nigeria. Electronic address: njoga.emmanuel@unn.edu.ng., Nwanta JA; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Nigeria, Nsukka 410001, Nigeria., Chah KF; Animal Health Antimicrobial Resistance Surveillance Sentinel Laboratory, Veterinary Teaching Hospital, University of Nigeria, Nsukka 410001, Nigeria; Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka 410001, Nigeria.
المصدر: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2023 Dec; Vol. 103, pp. 102083. Date of Electronic Publication: 2023 Oct 31.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 7808924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1667 (Electronic) Linking ISSN: 01479571 NLM ISO Abbreviation: Comp Immunol Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Publication: Exeter : Elsevier Science Ltd
Original Publication: Oxford, Elmsford, N. Y., Pergamon Press.
مواضيع طبية MeSH: Campylobacter*/genetics , One Health* , Campylobacter Infections*/epidemiology , Campylobacter Infections*/veterinary, Humans ; Animals ; Cattle ; Nigeria/epidemiology ; Public Health ; Drug Resistance, Bacterial ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Poultry ; Microbial Sensitivity Tests/veterinary
مستخلص: Antimicrobial-resistant thermophilic Campylobacter species (TCS) pose tremendous public health problems because they are zoonotic, difficult to treat and usually harboured by food-producing animals (FPAs). This study ascertained the phenotypic antimicrobial resistance (AMR) in 56 phenotypically identified TCS from slaughtered cattle, poultry, and humans in Enugu State, Nigeria. The presence of selected AMR and virulence genes harboured by the animal and human isolates were also detected and compared in 36 PCR-confirmed Campylobacter species. All the 56 TCS were multidrug-resistant as none were susceptible to ampicillin, penicillin-G, amoxicillin-clavulanic acid, cephalothin and metronidazole. The isolates were 92.9 %, 62.5 %, 92.9 %, 42.9 %, 26.8 %, 25 %, 28.6 %, 53.7 %, 30.1 %, 32.1 % and 55.4 % resistant to ceftriaxone, nalidixic acid, cefotaxime, enrofloxacin, ciprofloxacin, streptomycin, gentamycin, erythromycin, azithromycin, chloramphenicol and tetracycline, respectively. The top four most effective classes of antimicrobials were aminoglycosides > macrolides > amphenicol > fluoroquinolones. The AMR genes detected and the percentage of the isolates that harboured them were: aadE-1 (33.3 %), aphA-3-1 (36.1 %), tetO (44.4%), Bla oxa-61 (61.1 %) and the multidrug efflux pump, cmeB (86.1%). Virulence genes detected and the corresponding percentage of TCS that harboured them were: cdtB (61.1 %), flaA (47.2 %), ciaB (38.9 %), and pldA (38.9 %). The cmeB was significantly detected in animal isolates (p = 0.018, OR = 5.1, CI = 0.7-6.6) while Bla OXA-61 predominated in human isolates (p = 0.019, OR = 6.2). Likewise, ciaB virulence gene was mostly detected (p = 0.019, OR = 6.4, CI = 1.3-25) in animal isolates. The findings underscore the roles of FPAs in the zoonotic dissemination of Campylobacter-associated AMR and virulence genes in the study area. This warrants the adoption of One Health control strategies to limit spread of the multidrug-resistant zoonotic Campylobacter species.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Antimicrobial resistance genes; Campylobacter species; Food-producing animals; Humans; Multidrug resistance; Nigeria; Virulence genes
المشرفين على المادة: 0 (Anti-Bacterial Agents)
تواريخ الأحداث: Date Created: 20231105 Date Completed: 20231128 Latest Revision: 20231128
رمز التحديث: 20231128
DOI: 10.1016/j.cimid.2023.102083
PMID: 37925789
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-1667
DOI:10.1016/j.cimid.2023.102083