دورية أكاديمية
The effects of antimicrobial photodynamic therapy (aPDT) with nanotechnology-applied curcumin and 450nm blue led irradiation on multi-species biofilms in root canals.
| العنوان: | The effects of antimicrobial photodynamic therapy (aPDT) with nanotechnology-applied curcumin and 450nm blue led irradiation on multi-species biofilms in root canals. |
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| المؤلفون: | Ravazzi TPQ; Federal University of Bahia, Multidisciplinary Institute of Health, Rio de Contas Street, 58. Candeias, Vitória da Conquista, BA, 450029-094, Brazil. thayse.pithon@gmail.com., de Jesus IM; Federal University of Bahia, Multidisciplinary Institute of Health, Rio de Contas Street, 58. Candeias, Vitória da Conquista, BA, 450029-094, Brazil., de Oliveira Santos GP; Federal University of Bahia, Multidisciplinary Institute of Health, Rio de Contas Street, 58. Candeias, Vitória da Conquista, BA, 450029-094, Brazil., Reis TA; Federal University of Bahia, Multidisciplinary Institute of Health, Rio de Contas Street, 58. Candeias, Vitória da Conquista, BA, 450029-094, Brazil., Rosa LP; Federal University of Bahia, Multidisciplinary Institute of Health, Rio de Contas Street, 58. Candeias, Vitória da Conquista, BA, 450029-094, Brazil., Rosa FCS; Federal University of Bahia, Multidisciplinary Institute of Health, Rio de Contas Street, 58. Candeias, Vitória da Conquista, BA, 450029-094, Brazil. |
| المصدر: | Lasers in medical science [Lasers Med Sci] 2023 Nov 06; Vol. 38 (1), pp. 254. Date of Electronic Publication: 2023 Nov 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: England NLM ID: 8611515 Publication Model: Electronic Cited Medium: Internet ISSN: 1435-604X (Electronic) Linking ISSN: 02688921 NLM ISO Abbreviation: Lasers Med Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Springer Original Publication: London : Baillière Tindall, c1986- |
| مواضيع طبية MeSH: | Curcumin*/pharmacology , Methicillin-Resistant Staphylococcus aureus* , Photochemotherapy*/methods , Anti-Infective Agents*/pharmacology, Humans ; Dental Pulp Cavity ; Photosensitizing Agents/pharmacology ; Candida albicans ; Biofilms ; Escherichia coli |
| مستخلص: | This study aimed to evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) utilizing nanotechnology-applied curcumin activated by blue LED (450 nm) on the elimination of microorganisms arranged in multispecies biofilms inside the root canals of extracted human teeth. Forty single-rooted human teeth were used; these were randomized into four experimental groups, each comprising 10 teeth: control group, no treatment; photosensitizer (PS) group, nanotechnology-applied curcumin alone; light group, blue LED used separately; and aPDT group, nanotechnology-applied curcumin activated by blue LED. To carry out the tests, the interiors of the root canals were inoculated with species of Candida albicans (ATCC 90029), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), and methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 43300), using a multispecies biofilm. After the incubation period, the canals were treated according to the experimental groups, with no treatment given in the control group. Studied inasmuch as the antimicrobial effectiveness of aPDT was concerned, it was observed that the greatest reduction in microbial counts using aPDTs was achieved against MRSA (mean reduction = 2.48 Log10 CFU/mL), followed by Escherichia coli (mean reduction = 1.72), and Enterococcus faecalis (mean reduction = 1.65); a reduction greater than 1.5 Log10 CFU/mL showed relevant effectiveness of aPDT against these microorganisms. Of note, aPDT has also shown considerable effectiveness against Candida albicans (mean reduction = 0.71), with a statistical difference in the reduction between the groups. aPDT was effective in reducing all microorganisms examined. The average reduction was greater than 1.5 Log10 in all microorganisms except for Candida albicans. HIGHLIGHTS: • aPDT was a viable treatment for root canals; • Nanotechnological curcumin aPDT was effective in reducing multispecies biofilm microorganisms; • aPDT technique showed efficacy under the worst microbiological conditions , such as mature multispecies biofilm; • Nanotechnological curcumin aPDT was able to reduce Gram positive, negative bacterial and yeasts in root canals. (© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Biofilms; Curcumin; Endodontics; LED; Nanotechnology; Photodynamic therapy |
| المشرفين على المادة: | IT942ZTH98 (Curcumin) 0 (Anti-Infective Agents) 0 (Photosensitizing Agents) |
| تواريخ الأحداث: | Date Created: 20231106 Date Completed: 20231108 Latest Revision: 20231222 |
| رمز التحديث: | 20231222 |
| DOI: | 10.1007/s10103-023-03925-9 |
| PMID: | 37932526 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1435-604X |
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| DOI: | 10.1007/s10103-023-03925-9 |