دورية أكاديمية
Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis.
العنوان: | Pulicaria incisa (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis. |
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المؤلفون: | Alreshidi M; Department of Biology, College of Science, University of Ha'il, Hail 2440, Saudi Arabia., Abdulhakeem MA; Department of Biology, College of Science, University of Ha'il, Hail 2440, Saudi Arabia., Badraoui R; Department of Biology, College of Science, University of Ha'il, Hail 2440, Saudi Arabia.; Section of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia., Amato G; Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy., Caputo L; Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy., De Martino L; Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy., Nazzaro F; Institute of Food Sciences, CNR, 83100 Avellino, Italy., Fratianni F; Institute of Food Sciences, CNR, 83100 Avellino, Italy., Formisano C; Department of Pharmacy, School of Medicine and Surgery, University of Napoli Federico II, 80131 Napoli, Italy., De Feo V; Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy., Snoussi M; Department of Biology, College of Science, University of Ha'il, Hail 2440, Saudi Arabia.; Laboratory of Genetics, Biodiversity and Valorization of Bio-Resources (LR11ES41), Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Haddad, BP74, Monastir 5000, Tunisia. |
المصدر: | Molecules (Basel, Switzerland) [Molecules] 2023 Nov 05; Vol. 28 (21). Date of Electronic Publication: 2023 Nov 05. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, c1995- |
مواضيع طبية MeSH: | Antioxidants*/chemistry , Pulicaria*, Plant Extracts/pharmacology ; Plant Extracts/chemistry ; Anti-Bacterial Agents/pharmacology ; Phytochemicals/pharmacology ; Phytochemicals/chemistry ; Methanol |
مستخلص: | Plants with medicinal benefits are a crucial source of compounds for developing drugs. This study was designed to determine the chemical composition, antibacterial, antibiofilm, antioxidant, and anti-enzymatic activities of Pulicaria incisa (Lam.) DC. We also reported the molecular interaction between identified molecules and several receptors associated with antimicrobial and antibiofilm activities. A total of seventeen and thirteen compounds were identified in aqueous and methanolic extracts of P. incisa , respectively. The methanolic extract yielded a higher total content of polyphenols and flavonoids of about 84.80 ± 2.8 mg GAE/g and 28.30 ± 1.2 mg QE/g, respectively. Significant antibacterial activity was recorded for both extracts, with minimum inhibitory concentration (MIC) values ranging from 30 to 36 µg/mL, and the result was comparable to the reference antibiotic control. Antibiofilm assays revealed that both extracts were able to reduce the attachment of bacterial cells to 96-well plates, but the highest antibiofilm activity was recorded against Staphylococcus aureus . The methanolic extract also showed anti-enzymatic potency and high antioxidant activity, as demonstrated by all assays used, including DPPH, FRAP, and ABTS. These results were further validated by in silico approaches, particularly the molecular interaction of the identified compounds with the targeted receptors. These findings present P. incisa as a significant source of antibacterial, antibiofilm, antioxidant, and anti-enzymatic molecules. |
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فهرسة مساهمة: | Keywords: ADME; P. incisa extract; anti-enzymatic activities; antibacterial; antioxidant activities; molecular docking; phytochemical composition |
المشرفين على المادة: | 0 (Antioxidants) 0 (Plant Extracts) 0 (Anti-Bacterial Agents) 0 (Phytochemicals) Y4S76JWI15 (Methanol) |
تواريخ الأحداث: | Date Created: 20231114 Date Completed: 20231115 Latest Revision: 20231117 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC10648406 |
DOI: | 10.3390/molecules28217439 |
PMID: | 37959858 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1420-3049 |
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DOI: | 10.3390/molecules28217439 |