Long-term in vivo three-photon imaging reveals region-specific differences in healthy and regenerative oligodendrogenesis.
| العنوان: | Long-term in vivo three-photon imaging reveals region-specific differences in healthy and regenerative oligodendrogenesis. |
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| المؤلفون: | Thornton MA; Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus., Futia GL; Bioengineering, University of Colorado Anschutz Medical Campus., Stockton ME; Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus., Budoff SA; Physiology and Biophysics, University of Colorado Anschutz Medical Campus., Ramirez AN; Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus., Ozbay B; Intelligent Imaging Innovations (3i), Denver, CO, USA., Tzang O; Intelligent Imaging Innovations (3i), Denver, CO, USA., Kilborn K; Intelligent Imaging Innovations (3i), Denver, CO, USA., Poleg-Polsky A; Physiology and Biophysics, University of Colorado Anschutz Medical Campus., Restrepo D; Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus., Gibson EA; Bioengineering, University of Colorado Anschutz Medical Campus., Hughes EG; Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2023 Nov 01. Date of Electronic Publication: 2023 Nov 01. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | The generation of new myelin-forming oligodendrocytes in the adult CNS is critical for cognitive function and regeneration following injury. Oligodendrogenesis varies between gray and white matter regions suggesting that local cues drive regional differences in myelination and the capacity for regeneration. Yet, the determination of regional variability in oligodendrocyte cell behavior is limited by the inability to monitor the dynamics of oligodendrocytes and their transcriptional subpopulations in white matter of the living brain. Here, we harnessed the superior imaging depth of three-photon microscopy to permit long-term, longitudinal in vivo three-photon imaging of an entire cortical column and underlying subcortical white matter without cellular damage or reactivity. Using this approach, we found that the white matter generated substantially more new oligodendrocytes per volume compared to the gray matter, yet the rate of population growth was proportionally higher in the gray matter. Following demyelination, the white matter had an enhanced population growth that resulted in higher oligodendrocyte replacement compared to the gray matter. Finally, deep cortical layers had pronounced deficits in regenerative oligodendrogenesis and restoration of the MOL5/6-positive oligodendrocyte subpopulation following demyelinating injury. Together, our findings demonstrate that regional microenvironments regulate oligodendrocyte population dynamics and heterogeneity in the healthy and diseased brain. |
| التعليقات: | Update in: Nat Neurosci. 2024 Mar 27;:. (PMID: 38539013) |
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| معلومات مُعتمدة: | P30 NS048154 United States NS NINDS NIH HHS; P30 DK116073 United States DK NIDDK NIH HHS; R01 NS132859 United States NS NINDS NIH HHS; R01 NS118188 United States NS NINDS NIH HHS; R01 EY030841 United States EY NEI NIH HHS; R01 NS115975 United States NS NINDS NIH HHS; F31 NS120540 United States NS NINDS NIH HHS; UF1 NS116241 United States NS NINDS NIH HHS; R01 NS125230 United States NS NINDS NIH HHS |
| تواريخ الأحداث: | Date Created: 20231114 Latest Revision: 20240512 |
| رمز التحديث: | 20240513 |
| مُعرف محوري في PubMed: | PMC10634963 |
| DOI: | 10.1101/2023.10.29.564636 |
| PMID: | 37961298 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2023.10.29.564636 |
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