دورية أكاديمية
أعرض في EDS

The activity of engrailed imaginal disc enhancers is modulated epigenetically by chromatin and autoregulation.

التفاصيل البيبلوغرافية
العنوان: The activity of engrailed imaginal disc enhancers is modulated epigenetically by chromatin and autoregulation.
المؤلفون: Cheng Y; Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America., Chan F; Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America., Kassis JA; Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America.
المصدر: PLoS genetics [PLoS Genet] 2023 Nov 15; Vol. 19 (11), pp. e1010826. Date of Electronic Publication: 2023 Nov 15 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: Drosophila Proteins*/genetics , Drosophila Proteins*/metabolism , Imaginal Discs*/metabolism, Animals ; Chromatin/genetics ; Chromatin/metabolism ; DNA/metabolism ; Drosophila/genetics ; Epigenesis, Genetic ; Gene Expression Regulation, Developmental ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Homeostasis ; Transcription Factors/genetics ; Transcription Factors/metabolism
مستخلص: engrailed (en) encodes a homeodomain transcription factor crucial for the proper development of Drosophila embryos and adults. Like many developmental transcription factors, en expression is regulated by many enhancers, some of overlapping function, that drive expression in spatially and temporally restricted patterns. The en embryonic enhancers are located in discrete DNA fragments that can function correctly in small reporter transgenes. In contrast, the en imaginal disc enhancers (IDEs) do not function correctly in small reporter transgenes. En is expressed in the posterior compartment of wing imaginal discs; in contrast, small IDE-reporter transgenes are expressed mainly in the anterior compartment. We found that En binds to the IDEs and suggest that it may directly repress IDE function and modulate En expression levels. We identified two en IDEs, O and S. Deletion of either of these IDEs from a 79kb HA-en rescue transgene (HAen79) caused a loss-of-function en phenotype when the HAen79 transgene was the sole source of En. In contrast, flies with a deletion of the same IDEs from an endogenous en gene had no phenotype, suggesting a resiliency not seen in the HAen79 rescue transgene. Inserting a gypsy insulator in HAen79 between en regulatory DNA and flanking sequences strengthened the activity of HAen79, giving better function in both the ON and OFF transcriptional states. Altogether our data suggest that the en IDEs stimulate expression in the entire imaginal disc, and that the ON/OFF state is set by epigenetic memory set by the embryonic enhancers. This epigenetic regulation is similar to that of the Ultrabithorax IDEs and we suggest that the activity of late-acting enhancers in other genes may be similarly regulated.
Competing Interests: The authors declare no competing interests.
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
التعليقات: Update of: bioRxiv. 2023 Jun 16;:. (PMID: 37502849)
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المشرفين على المادة: 0 (Chromatin)
9007-49-2 (DNA)
0 (Drosophila Proteins)
0 (Homeodomain Proteins)
0 (Transcription Factors)
0 (En protein, Drosophila)
تواريخ الأحداث: Date Created: 20231115 Date Completed: 20231204 Latest Revision: 20231204
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10686433
DOI: 10.1371/journal.pgen.1010826
PMID: 37967127
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7404
DOI:10.1371/journal.pgen.1010826