دورية أكاديمية
Whole genome sequencing of drug-resistant Mycobacterium tuberculosis isolates in Victoria, Australia.
| العنوان: | Whole genome sequencing of drug-resistant Mycobacterium tuberculosis isolates in Victoria, Australia. |
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| المؤلفون: | Dorji T; Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia., Horan K; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia., Sherry NL; Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia., Tay EL; Communicable Disease Epidemiology and Surveillance, Health Protection Branch, Public Health Division, Department of Health, Melbourne, Australia., Globan M; Mycobacterium Reference Laboratory, Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia., Viberg L; Mycobacterium Reference Laboratory, Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia., Bond K; Mycobacterium Reference Laboratory, Victorian Infectious Diseases Reference Laboratory, Royal Melbourne Hospital at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia; Royal Melbourne Hospital, Melbourne, Australia., Denholm JT; Royal Melbourne Hospital, Melbourne, Australia; Victorian Tuberculosis Program. Melbourne Health at the Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia; Department of Infectious Diseases at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia., Howden BP; Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia; Centre for Pathogen Genomics, University of Melbourne, Australia. Electronic address: bhowden@unimelb.edu.au., Andersson P; Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology at The Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, Australia. |
| المصدر: | International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2024 Jan; Vol. 138, pp. 46-53. Date of Electronic Publication: 2023 Nov 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Canada NLM ID: 9610933 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3511 (Electronic) Linking ISSN: 12019712 NLM ISO Abbreviation: Int J Infect Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Hamilton, ON : Elsevier Original Publication: Hamilton, ON : Decker, c1996- |
| مواضيع طبية MeSH: | Mycobacterium tuberculosis* , Tuberculosis, Multidrug-Resistant*/drug therapy , Tuberculosis, Multidrug-Resistant*/epidemiology , Tuberculosis, Multidrug-Resistant*/microbiology, Humans ; Antitubercular Agents/pharmacology ; Antitubercular Agents/therapeutic use ; Isoniazid/pharmacology ; Isoniazid/therapeutic use ; Victoria/epidemiology ; Phylogeny ; Retrospective Studies ; Whole Genome Sequencing ; Mutation ; Microbial Sensitivity Tests ; Drug Resistance, Multiple, Bacterial/genetics |
| مستخلص: | Objectives: Whole genome sequencing (WGS) can identify clusters, transmission patterns, and drug resistance mutations. This is important in low-burden settings such as Australia, as it can assist in efficient contact tracing and surveillance. Methods: We conducted a retrospective cohort study using WGS from 155 genomically defined drug-resistant Mycobacterium tuberculosis (DR-TB) isolates collected between 2018-2021 in Victoria, Australia. Bioinformatic analysis was performed to identify resistance-conferring mutations, lineages, clusters and understand how local sequences compared with international context. Results: Of the 155 sequences, 42% were identified as lineage 2 and 35% as lineage 1; 65.8% (102/155) were isoniazid mono-resistant, 8.4% were multi-drug resistant TB and 5.8% were pre-extensively drug-resistant / extensively drug-resistant TB. The most common mutations were observed in katG and fabG1 genes, especially at Ser315Thr and fabG1 -15 C>T for first-line drugs. Ser450Leu was the most frequent mutation in rpoB gene. Phylogenetic analysis confirmed that Victorian DR-TB were associated with importation events. There was little evidence of local transmission with only five isolate pairs. Conclusion: Isoniazid-resistant TB is the commonest DR-TB in Victoria, and the mutation profile is similar to global circulating DR-TB. Most cases are diagnosed among migrants with limited transmission. This study highlights the value of WGS in identification of clusters and resistance-conferring mutations. This information is crucial in supporting disease mitigation and treatment strategies. Competing Interests: Declarations of competing interest The authors have no competing interests to declare. (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Australia; Genomic epidemiology; MDR-TB; Multi-drug resistant tuberculosis; Transmission networks |
| المشرفين على المادة: | 0 (Antitubercular Agents) V83O1VOZ8L (Isoniazid) |
| تواريخ الأحداث: | Date Created: 20231115 Date Completed: 20231218 Latest Revision: 20231218 |
| رمز التحديث: | 20231218 |
| DOI: | 10.1016/j.ijid.2023.11.010 |
| PMID: | 37967715 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1878-3511 |
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| DOI: | 10.1016/j.ijid.2023.11.010 |