دورية أكاديمية
أعرض في EDS

Isolation, adaptation, and characterization of lumpy skin disease virus from cattle in Bangladesh.

التفاصيل البيبلوغرافية
العنوان: Isolation, adaptation, and characterization of lumpy skin disease virus from cattle in Bangladesh.
المؤلفون: Pervin S; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Ahamed MM; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Chouhan CS; Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Jahan MS; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Ahmed R; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Nazmul KHM; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Nazir H; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Siddique MP; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Rahman MT; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Kafi MA; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh., Rahman MB; Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
المصدر: Journal of advanced veterinary and animal research [J Adv Vet Anim Res] 2023 Sep 30; Vol. 10 (3), pp. 563-569. Date of Electronic Publication: 2023 Sep 30 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Network for the Veterinarians of Bangladesh Country of Publication: Bangladesh NLM ID: 101647585 Publication Model: eCollection Cited Medium: Internet ISSN: 2311-7710 (Electronic) Linking ISSN: 23117710 NLM ISO Abbreviation: J Adv Vet Anim Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Mymensingh : Network for the Veterinarians of Bangladesh, [2014]-
مستخلص: Objective: The research aimed to isolate, adapt to cell culture, and characterize the lumpy skin disease virus (LSDV) from clinically infected cattle in Bangladesh.
Materials and Methods: From September 2019 to June 2020, 37 skin nodules and skin swabs were aseptically collected from afflicted cattle in the outbreak regions of Jhenaidah and Kishoreganj in Bangladesh. The LSDV was isolated from embryonated specific pathogen-free (SPF) chicken eggs along the chorioallantoic membrane (CAM) route and the Vero cell line after several blind passages. The viral attachment protein was targeted for molecular detection using polymerase chain reactions (PCR). For phylogenetic analysis, PCR-positive products were partially sequenced.
Results: The virus was evident in the cell line, showed cytopathic effects after the 13 blind passage, and on the CAM of SPF chicken eggs, exhibited thickening of the CAM with pock-like lesions. A total of 12 samples (32.43%) tested positive for LSDV by PCR. Phylogenetic analysis of the present isolates (accession numbers MN792649 and MN792650) revealed 100% similarity with strains from India (MN295064), Kenya (AF325528, MN072619, KX683219), Greece (KY829023), Serbia (KY702007), and Kazakhstan (MN642592); moreover, 99.43% to 100% similarity to the sheep pox virus.
Conclusion: Partially sequenced LSDV was developed as a vaccine seed and was first isolated in Bangladesh and characterized at the molecular level.
Competing Interests: The researchers do not possess any competing priorities to disclose.
(Copyright: © Journal of Advanced Veterinary and Animal Research.)
References: J Virol Methods. 2020 Nov;285:113943. (PMID: 32707050)
Genome Announc. 2017 Aug 31;5(35):. (PMID: 28860246)
Arch Virol. 2003 Jul;148(7):1335-56. (PMID: 12827464)
Genome Announc. 2017 Jul 20;5(29):. (PMID: 28729259)
Genome Announc. 2018 May 31;6(22):. (PMID: 29853513)
J Virol Methods. 1998 Sep;74(1):1-7. (PMID: 9763122)
J Virol. 2001 Aug;75(15):7122-30. (PMID: 11435593)
Evolution. 1985 Jul;39(4):783-791. (PMID: 28561359)
J Virol Methods. 2008 Aug;151(2):225-229. (PMID: 18582954)
Transbound Emerg Dis. 2020 Jan;67(1):80-97. (PMID: 31379093)
Transbound Emerg Dis. 2021 Mar;68(2):216-219. (PMID: 33119963)
Viruses. 2022 Nov 15;14(11):. (PMID: 36423138)
Vet Med Sci. 2021 May;7(3):888-896. (PMID: 33522708)
Vet Med Int. 2021 Feb 4;2021:8862180. (PMID: 33708372)
BMC Vet Res. 2021 Jan 29;17(1):61. (PMID: 33514360)
Viruses. 2022 Oct 07;14(10):. (PMID: 36298758)
Microb Pathog. 2021 Sep;158:105050. (PMID: 34146642)
Transbound Emerg Dis. 2018 Jun;65(3):916-920. (PMID: 29363284)
Microorganisms. 2022 Apr 25;10(5):. (PMID: 35630342)
Transbound Emerg Dis. 2022 Nov;69(6):3405-3418. (PMID: 36056232)
Mol Biol Evol. 1987 Jul;4(4):406-25. (PMID: 3447015)
Transbound Emerg Dis. 2020 Nov;67(6):2408-2422. (PMID: 32304275)
PLoS One. 2021 Jan 11;16(1):e0241022. (PMID: 33428633)
Vet World. 2019 Jul;12(7):1093-1100. (PMID: 31528038)
Transbound Emerg Dis. 2020 Nov;67(6):2280-2281. (PMID: 32889764)
فهرسة مساهمة: Keywords: Bangladesh; MDBK cell line; Vero cell line; cattle; lumpy skin disease virus; viral attachment protein gene
تواريخ الأحداث: Date Created: 20231116 Latest Revision: 20231117
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10636076
DOI: 10.5455/javar.2023.j710
PMID: 37969804
قاعدة البيانات: MEDLINE
الوصف
تدمد:2311-7710
DOI:10.5455/javar.2023.j710