دورية أكاديمية
Assessment of inter-operator variability in peripheral monocyte subset gating strategy using flow cytometry in patients with suspected acute stroke.
| العنوان: | Assessment of inter-operator variability in peripheral monocyte subset gating strategy using flow cytometry in patients with suspected acute stroke. |
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| المؤلفون: | Heng E; INSERM, Innovative Therapies in Haemostasis, Université Paris Cité, Paris, France.; Service d'Hématologie Biologique, Hôpital Lariboisière, APHP.Nord, Paris, France., Neuwirth M; INSERM, Innovative Therapies in Haemostasis, Université Paris Cité, Paris, France.; Service d'Hématologie Biologique, Hôpital Lariboisière, APHP.Nord, Paris, France., Mas F; R&D Hematology and Hemostasis department, Biocytex, Marseille, France., Contant G; Recherche prospective, Diagnostica Stago, Gennevilliers, France., Mazighi M; Unité Neuro-vasculaire, Hôpital Lariboisière, APHP.Nord, Paris, France., Feriel J; Développement clinique, Diagnostica Stago, Asnières-sur-Seine, France., Montpellier B; R&D Hematology and Hemostasis department, Biocytex, Marseille, France., Brumpt C; Service d'Hématologie Biologique, Hôpital Lariboisière, APHP.Nord, Paris, France., Jourdi G; INSERM, Innovative Therapies in Haemostasis, Université Paris Cité, Paris, France.; Service d'Hématologie Biologique, Hôpital Lariboisière, APHP.Nord, Paris, France., Curis E; Service d'Hématologie Biologique, Hôpital Lariboisière, APHP.Nord, Paris, France.; UR 7537 BioSTM (Biostatistics), Faculté de Pharmacie de Paris, Université Paris Cité, Paris, France., Siguret V; INSERM, Innovative Therapies in Haemostasis, Université Paris Cité, Paris, France.; Service d'Hématologie Biologique, Hôpital Lariboisière, APHP.Nord, Paris, France. |
| المصدر: | Cytometry. Part A : the journal of the International Society for Analytical Cytology [Cytometry A] 2024 Mar; Vol. 105 (3), pp. 171-180. Date of Electronic Publication: 2023 Nov 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 101235694 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4930 (Electronic) Linking ISSN: 15524922 NLM ISO Abbreviation: Cytometry A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hoboken, N.J. : Wiley-Liss, c2002- |
| مواضيع طبية MeSH: | Monocytes*/metabolism , HLA-DR Antigens*, Humans ; Flow Cytometry ; Biomarkers/metabolism |
| مستخلص: | Background: Innovative tools to reliably identify patients with acute stroke are needed. Peripheral monocyte subsets, that is, classical-Mon1, intermediate-Mon2, and non-classical-Mon3, with their activation marker expression analyzed using flow-cytometry (FCM) could be interesting cell biomarker candidates. Aim: To assess the inter-operator variability in a new peripheral monocyte subset gating strategy using FCM in patients with suspected acute stroke. Methods: In BOOST-study ("Biomarkers-algOrithm-for-strOke-diagnoSis-and Treatment-resistance-prediction," NCT04726839), patients ≥18 years with symptoms suggesting acute stroke within the last 24 h were included. Blood was collected upon admission to emergency unit. FCM analysis was performed using the FACS-CANTO-II® flow-cytometer and Flow-Jo™-software. Analyzed markers were CD45/CD91/CD14/CD16 (monocyte backbone) and CD62L/CD11b/HLA-DR/CD86/CCR2/ICAM-1/CX3CR1/TF (activation markers). Inter-operator agreement (starting from raw-data files) was quantified by the measure distribution and, for each patient, the coefficient of variation (CV). Results: Three operators analyzed 20 patient blood samples. Median inter-operator CVs were below the pre-specified tolerance limits (10% [for Mon1 counts], 20% [Mon2, Mon3 counts], 15% [activation marker median-fluorescence-intensities]). We observed a slight, but systematic, inter-operator effect. Overall, absolute inter-operator differences in fractions of monocyte subsets were <0.03. Conclusion: Our gating strategy allowed monocyte subset gating with an acceptable inter-operator variability. Although low, the inter-operator effect should be considered in monocyte data analysis of BOOST-patients. (© 2023 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.) |
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| معلومات مُعتمدة: | ANR-18-RHUS-0001 Agence Nationale de la Recherche; Biocytex |
| فهرسة مساهمة: | Keywords: activation markers; flow cytometry; inter-operator variability; monocyte; stroke |
| المشرفين على المادة: | 0 (HLA-DR Antigens) 0 (Biomarkers) |
| تواريخ الأحداث: | Date Created: 20231116 Date Completed: 20240315 Latest Revision: 20240408 |
| رمز التحديث: | 20240408 |
| DOI: | 10.1002/cyto.a.24810 |
| PMID: | 37971361 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1552-4930 |
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| DOI: | 10.1002/cyto.a.24810 |