دورية أكاديمية
Phosphodiesterase 4 activity uniquely regulates ciliary cAMP-dependent 3T3-L1 adipogenesis.
| العنوان: | Phosphodiesterase 4 activity uniquely regulates ciliary cAMP-dependent 3T3-L1 adipogenesis. |
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| المؤلفون: | Erdelsky MR; Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada., Groves SA; Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada., Shah C; Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada., Delios SB; Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada., Umana MB; Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada., Maurice DH; Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada. Electronic address: mauriced@queensu.ca. |
| المصدر: | Cellular signalling [Cell Signal] 2024 Jan; Vol. 113, pp. 110981. Date of Electronic Publication: 2023 Nov 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 8904683 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3913 (Electronic) Linking ISSN: 08986568 NLM ISO Abbreviation: Cell Signal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Science Ltd Original Publication: Oxford ; New York : Pergamon Press, 1988- |
| مواضيع طبية MeSH: | Adipogenesis* , Cyclic Nucleotide Phosphodiesterases, Type 4*, Mice ; Animals ; 3T3-L1 Cells ; Cell Differentiation ; Docosahexaenoic Acids ; PPAR gamma |
| مستخلص: | Recent evidence indicates that the presence of a primary cilium (PC), and of selective cAMP signaling within this smallest of organelles, promotes adipogenic differentiation of 3T3-L1 preadipocytes incubated in media supplemented with either a natural (docosahexaenoic acid, DHA), or a synthetic (TUG-891), free fatty acid receptor 4 (FFAR4) agonist. Indeed, in this earlier work, activation of ciliary FFAR4 in 3T3-L1 cells was correlated with selective increases in PC cAMP and adipogenesis in these cells. However, this study was silent on the role of local PC cAMP phosphodiesterases (PDEs)-mediated events in regulating these adipogenic responses and on the identity of cAMP PDEs that could regulate the "pool" of ciliary cAMP accessed by FFAR4 agonists. In this context, we have identified the PDEs expressed by 3T3-L1 preadipocytes and showed that of these, only PDE4 inhibition promotes FFAR4-mediated adipogenesis. We propose that this work will identify more selective therapeutic targets through which to control adipogenesis, and perhaps the differentiation of other stem cells in which ciliary cAMP is critical. Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Donald Maurice reports financial support was provided by Canadian Institutes of Health Research. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Adipogenesis; Primary cilium; cAMP |
| المشرفين على المادة: | EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 4) 47341-71-9 (diethylstilbestrol monophosphate) 25167-62-8 (Docosahexaenoic Acids) 0 (PPAR gamma) |
| تواريخ الأحداث: | Date Created: 20231119 Date Completed: 20231204 Latest Revision: 20240322 |
| رمز التحديث: | 20240323 |
| DOI: | 10.1016/j.cellsig.2023.110981 |
| PMID: | 37981066 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3913 |
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| DOI: | 10.1016/j.cellsig.2023.110981 |