دورية أكاديمية
Sensitive detection of viable Cronobacter sakazakii by bioluminescent reporter phage emitting stable signals with truncated holin.
| العنوان: | Sensitive detection of viable Cronobacter sakazakii by bioluminescent reporter phage emitting stable signals with truncated holin. |
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| المؤلفون: | Kim D; Laboratory of Molecular Food Microbiology, Department of Food and Nutrition, Brain Korea 21 FOUR, College of Human Ecology, Yonsei University, Seoul 03722, Republic of Korea., Kim M; Laboratory of Molecular Food Microbiology, Department of Food and Nutrition, Brain Korea 21 FOUR, College of Human Ecology, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: m.kim@yonsei.ac.kr. |
| المصدر: | Food research international (Ottawa, Ont.) [Food Res Int] 2023 Dec; Vol. 174 (Pt 2), pp. 113665. Date of Electronic Publication: 2023 Nov 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| مواضيع طبية MeSH: | Cronobacter sakazakii*/genetics , Cronobacter sakazakii*/isolation & purification , Bacteriological Techniques*/methods , Bacteriophages*/genetics , Bacteriophages*/metabolism , Viral Proteins*/genetics , Viral Proteins*/metabolism , Food Microbiology*/methods , Luminescent Measurements*/methods, Genome, Viral/genetics ; Sequence Deletion ; Sensitivity and Specificity |
| مستخلص: | As outbreaks of foodborne illness caused by the opportunistic pathogen Cronobacter sakazakii (Cs) continue to occur, particularly in infants consuming powdered infant formula (PIF), the need for sensitive, rapid, and easy-to-use detection of Cs from food and food processing environments is increasing. Here, we developed bioluminescent reporter bacteriophages for viable Cs-specific, substrate-free, rapid detection by introducing luciferase and its corresponding substrate-providing enzyme complex into the virulent phage ΦC01. Although the reporter phage ΦC01_lux, constructed by replacing non-essential genes for phage infectivity with a luxCDABE reporter operon, produced bioluminescence upon Cs infection, the emitted signal was quickly decayed due to the superior bacteriolytic activity of ΦC01. By truncating the membrane pore-forming protein holin and thus limiting its function, the bacterial lysis was delayed and the resultant engineered reporter phage ΦC01_lux_Δhol could produce a more stable and reliable bioluminescent signal. Accordingly, ΦC01_lux_Δhol was able to detect at least an average of 2 CFU/ml of Cs artificially contaminated PIF and Sunsik and food contact surface models within a total of 7 h of assays, including 5 h of pre-enrichment for Cs amplification. The sensitive, easy-to-use, and specific detection of live Cs with the developed reporter phage could be applied as a novel complementary tool for monitoring Cs in food and food-related environments for food safety and public health. Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: D.K and M.K. have submitted a patent application covering this research. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bacteriophage; Bioluminescence; Cronobacter sakazakii; Holin; Pathogen detection; Powdered infant formula; Reporter phage |
| المشرفين على المادة: | 0 (Viral Proteins) |
| تواريخ الأحداث: | Date Created: 20231119 Date Completed: 20240201 Latest Revision: 20240201 |
| رمز التحديث: | 20240201 |
| DOI: | 10.1016/j.foodres.2023.113665 |
| PMID: | 37981373 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-7145 |
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| DOI: | 10.1016/j.foodres.2023.113665 |