دورية أكاديمية
Fourth Chromosome Resource Project: a comprehensive resource for genetic analysis in Drosophila that includes humanized stocks.
| العنوان: | Fourth Chromosome Resource Project: a comprehensive resource for genetic analysis in Drosophila that includes humanized stocks. |
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| المؤلفون: | Stinchfield MJ; School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501, USA., Weasner BP; Department Biology, Indiana University, Bloomington, IN 47405, USA., Weasner BM; Department Biology, Indiana University, Bloomington, IN 47405, USA., Zhitomersky D; Department Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA., Kumar JP; Department Biology, Indiana University, Bloomington, IN 47405, USA., O'Connor MB; Department Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN 55455, USA., Newfeld SJ; School of Life Sciences, Arizona State University, Tempe, AZ 85287-4501, USA. |
| المصدر: | Genetics [Genetics] 2024 Feb 07; Vol. 226 (2). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0374636 Publication Model: Print Cited Medium: Internet ISSN: 1943-2631 (Electronic) Linking ISSN: 00166731 NLM ISO Abbreviation: Genetics Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Austin, Tex. [etc.] |
| مواضيع طبية MeSH: | Drosophila*/genetics , Chromosomes*, Animals ; Humans ; Mutagenesis ; Drosophila melanogaster/genetics |
| مستخلص: | The fourth chromosome is the final frontier for genetic analysis in Drosophila. Small, heterochromatic, and devoid of recombination the fourth has long been ignored. Nevertheless, its long arm contains 79 protein-coding genes. The Fourth Chromosome Resource Project (FCRP) has a goal of facilitating the investigation of genes on this neglected chromosome. The project has 446 stocks publicly available at the Bloomington and Kyoto stock centers with phenotypic data curated by the FlyBase and FlyPush resources. Four of the five stock sets are nearly complete: (1) UAS.fly cDNAs, (2) UAS.human homolog cDNAs, (3) gene trap mutants and protein traps, and (4) stocks promoting meiotic and mitotic recombination on the fourth. Ongoing is mutagenesis of each fourth gene on a new FRT-bearing chromosome for marked single-cell clones. Beyond flies, FCRP facilitates the creation and analysis of humanized fly stocks. These provide opportunities to apply Drosophila genetics to the analysis of human gene interaction and function. In addition, the FCRP provides investigators with confidence through stock validation and an incentive via phenotyping to tackle genes on the fourth that have never been studied. Taken together, FCRP stocks will facilitate all manner of genetic and molecular studies. The resource is readily available to researchers to enhance our understanding of metazoan biology, including conserved molecular mechanisms underlying health and disease. Competing Interests: Conflicts of interest All authors declare no conflict of interest. (© The Author(s) 2023. Published by Oxford University Press on behalf of The Genetics Society of America.) |
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| معلومات مُعتمدة: | P40 OD010949 United States OD NIH HHS; P40 OD018537 United States OD NIH HHS; R35 GM118029 United States GM NIGMS NIH HHS; OD028242 United States GF NIH HHS; R24 OD028242 United States OD NIH HHS; R01 EY030847 United States EY NEI NIH HHS |
| فهرسة مساهمة: | Keywords: Activin/Inhibin; gene/protein traps; larval/adult brains |
| تواريخ الأحداث: | Date Created: 20231120 Date Completed: 20240208 Latest Revision: 20240701 |
| رمز التحديث: | 20240701 |
| مُعرف محوري في PubMed: | PMC10847715 |
| DOI: | 10.1093/genetics/iyad201 |
| PMID: | 37981656 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1943-2631 |
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| DOI: | 10.1093/genetics/iyad201 |