دورية أكاديمية
Lactate- and immunomagnetic-purified hiPSC-derived cardiomyocytes generate comparable engineered cardiac tissue constructs.
| العنوان: | Lactate- and immunomagnetic-purified hiPSC-derived cardiomyocytes generate comparable engineered cardiac tissue constructs. |
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| المؤلفون: | Rossler KJ; Molecular and Cellular Pharmacology Training Program.; Department of Cell and Regenerative Biology., de Lange WJ; Department of Pediatrics., Mann MW; Department of Medicine., Aballo TJ; Molecular and Cellular Pharmacology Training Program.; Department of Cell and Regenerative Biology., Melby JA; Department of Chemistry, and., Zhang J; Department of Medicine., Kim G; Department of Medicine., Bayne EF; Department of Chemistry, and., Zhu Y; Human Proteomics Program, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA., Farrell ET; Department of Pediatrics., Kamp TJ; Department of Cell and Regenerative Biology.; Department of Medicine., Ralphe JC; Department of Pediatrics., Ge Y; Department of Cell and Regenerative Biology.; Department of Chemistry, and.; Human Proteomics Program, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin, USA. |
| المصدر: | JCI insight [JCI Insight] 2024 Jan 09; Vol. 9 (1). Date of Electronic Publication: 2024 Jan 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Clinical Investigation Country of Publication: United States NLM ID: 101676073 Publication Model: Electronic Cited Medium: Internet ISSN: 2379-3708 (Electronic) Linking ISSN: 23793708 NLM ISO Abbreviation: JCI Insight Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Ann Arbor, Michigan : American Society for Clinical Investigation, [2016]- |
| مواضيع طبية MeSH: | Myocytes, Cardiac*/metabolism , Induced Pluripotent Stem Cells*/metabolism, Humans ; Lactic Acid/metabolism ; Tissue Engineering ; Proteomics ; Cells, Cultured |
| مستخلص: | Three-dimensional engineered cardiac tissue (ECT) using purified human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) has emerged as an appealing model system for the study of human cardiac biology and disease. A recent study reported widely used metabolic (lactate) purification of monolayer hiPSC-CM cultures results in an ischemic cardiomyopathy-like phenotype compared with magnetic antibody-based cell sorting (MACS) purification, complicating the interpretation of studies using lactate-purified hiPSC-CMs. Herein, our objective was to determine if use of lactate relative to MACS-purified hiPSC-CMs affects the properties of resulting hiPSC-ECTs. Therefore, hiPSC-CMs were differentiated and purified using either lactate-based media or MACS. Global proteomics revealed that lactate-purified hiPSC-CMs displayed a differential phenotype over MACS hiPSC-CMs. hiPSC-CMs were then integrated into 3D hiPSC-ECTs and cultured for 4 weeks. Structurally, there was no significant difference in sarcomere length between lactate and MACS hiPSC-ECTs. Assessment of isometric twitch force and Ca2+ transient measurements revealed similar functional performance between purification methods. High-resolution mass spectrometry-based quantitative proteomics showed no significant difference in protein pathway expression or myofilament proteoforms. Taken together, this study demonstrates that lactate- and MACS-purified hiPSC-CMs generate ECTs with comparable structural, functional, and proteomic features, and it suggests that lactate purification does not result in an irreversible change in a hiPSC-CM phenotype. |
| التعليقات: | Update of: bioRxiv. 2023 May 06;:. (PMID: 37205556) |
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| معلومات مُعتمدة: | U01 HL134764 United States HL NHLBI NIH HHS; P30 CA014520 United States CA NCI NIH HHS; S10 OD023526 United States OD NIH HHS; R01 HL109810 United States HL NHLBI NIH HHS; R01 HL096971 United States HL NHLBI NIH HHS; R01 GM125085 United States GM NIGMS NIH HHS; S10 OD018475 United States OD NIH HHS |
| فهرسة مساهمة: | Keywords: Cardiology; Cardiovascular disease; Stem cells; iPS cells |
| المشرفين على المادة: | 33X04XA5AT (Lactic Acid) |
| تواريخ الأحداث: | Date Created: 20231121 Date Completed: 20240110 Latest Revision: 20240303 |
| رمز التحديث: | 20240303 |
| مُعرف محوري في PubMed: | PMC10906451 |
| DOI: | 10.1172/jci.insight.172168 |
| PMID: | 37988170 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2379-3708 |
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| DOI: | 10.1172/jci.insight.172168 |