دورية أكاديمية
Identification of NAD-RNA species and ADPR-RNA decapping in Archaea.
| العنوان: | Identification of NAD-RNA species and ADPR-RNA decapping in Archaea. |
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| المؤلفون: | Gomes-Filho JV; Faculty of Biology, Philipps-Universität Marburg, Marburg, Germany. gomesfil@staff.uni-marburg.de., Breuer R; Faculty of Biology, Philipps-Universität Marburg, Marburg, Germany., Morales-Filloy HG; Institute of Pharmacy and Molecular Biotechnology (IPMB), Heidelberg University, Heidelberg, Germany., Pozhydaieva N; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Borst A; Institute for Molecular Biosciences, Biocentre, Goethe-University, Frankfurt am Main, Germany., Paczia N; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany., Soppa J; Institute for Molecular Biosciences, Biocentre, Goethe-University, Frankfurt am Main, Germany., Höfer K; Max Planck Institute for Terrestrial Microbiology, Marburg, Germany.; SYNMIKRO, Center for Synthetic Microbiology, Marburg, Germany., Jäschke A; Institute of Pharmacy and Molecular Biotechnology (IPMB), Heidelberg University, Heidelberg, Germany., Randau L; Faculty of Biology, Philipps-Universität Marburg, Marburg, Germany. randau@staff.uni-marburg.de.; SYNMIKRO, Center for Synthetic Microbiology, Marburg, Germany. randau@staff.uni-marburg.de. |
| المصدر: | Nature communications [Nat Commun] 2023 Nov 21; Vol. 14 (1), pp. 7597. Date of Electronic Publication: 2023 Nov 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | RNA* , NAD*/metabolism, Adenosine Diphosphate Ribose/metabolism ; Archaea/metabolism ; Chromatography, Liquid ; Tandem Mass Spectrometry |
| مستخلص: | NAD is a coenzyme central to metabolism that also serves as a 5'-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archaeon Sulfolobus acidocaldarius and in the halophilic mesophile Haloferax volcanii. None of the four Nudix proteins of S. acidocaldarius catalyze NAD-RNA decapping in vitro, but one of the proteins (Saci_NudT5) promotes ADPR-RNA decapping. NAD-RNAs are converted into ADPR-RNAs, which we detect in S. acidocaldarius total RNA. Deletion of the gene encoding the 5'-3' exonuclease Saci-aCPSF2 leads to a 4.5-fold increase in NAD-RNA levels. We propose that the incorporation of NAD into RNA acts as a degradation marker for Saci-aCPSF2. In contrast, ADPR-RNA is processed by Saci_NudT5 into 5'-p-RNAs, providing another layer of regulation for RNA turnover in archaeal cells. (© 2023. The Author(s).) |
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| معلومات مُعتمدة: | 882789 EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council) |
| المشرفين على المادة: | 63231-63-0 (RNA) 0U46U6E8UK (NAD) 20762-30-5 (Adenosine Diphosphate Ribose) |
| تواريخ الأحداث: | Date Created: 20231121 Date Completed: 20231123 Latest Revision: 20231124 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10663502 |
| DOI: | 10.1038/s41467-023-43377-x |
| PMID: | 37989750 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/s41467-023-43377-x |