دورية أكاديمية
Inter-Organellar Effects of Defective ER-Localized Linolenic Acid Formation on Thylakoid Lipid Composition, Non-Photochemical Quenching of Chlorophyll Fluorescence and Xanthophyll Cycle Activity in the Arabidopsis fad3 Mutant.
| العنوان: | Inter-Organellar Effects of Defective ER-Localized Linolenic Acid Formation on Thylakoid Lipid Composition, Non-Photochemical Quenching of Chlorophyll Fluorescence and Xanthophyll Cycle Activity in the Arabidopsis fad3 Mutant. |
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| المؤلفون: | Matzner M; Department of Plant Biochemistry, Institute of Biochemistry and Biotechnology, Charles Tanford Protein Science Center, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3a, Halle (Saale) 06120, Germany., Launhardt L; Department of Plant Biochemistry, Institute of Biochemistry and Biotechnology, Charles Tanford Protein Science Center, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3a, Halle (Saale) 06120, Germany., Barth O; Department of Plant Physiology, Institute of Biology, Martin Luther University Halle-Wittenberg, Weinbergweg 10, Halle (Saale) 06120, Germany., Humbeck K; Department of Plant Physiology, Institute of Biology, Martin Luther University Halle-Wittenberg, Weinbergweg 10, Halle (Saale) 06120, Germany., Goss R; Department of Plant Physiology, Institute of Biology, University of Leipzig, Johannisallee 23, Leipzig 04103, Germany., Heilmann I; Department of Plant Biochemistry, Institute of Biochemistry and Biotechnology, Charles Tanford Protein Science Center, Martin Luther University Halle-Wittenberg, Kurt-Mothes-Str. 3a, Halle (Saale) 06120, Germany. |
| المصدر: | Plant & cell physiology [Plant Cell Physiol] 2024 Jun 27; Vol. 65 (6), pp. 958-974. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: Japan NLM ID: 9430925 Publication Model: Print Cited Medium: Internet ISSN: 1471-9053 (Electronic) Linking ISSN: 00320781 NLM ISO Abbreviation: Plant Cell Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tokyo : Oxford University Press Original Publication: Kyoto, Japan : Japanese Society of Plant Physiologists, |
| مواضيع طبية MeSH: | Arabidopsis*/genetics , Arabidopsis*/metabolism , Arabidopsis*/drug effects , Thylakoids*/metabolism , Endoplasmic Reticulum*/metabolism , alpha-Linolenic Acid*/metabolism , Arabidopsis Proteins*/metabolism , Arabidopsis Proteins*/genetics , Galactolipids*/metabolism , Chlorophyll*/metabolism , Xanthophylls*/metabolism , Mutation*/genetics, Fatty Acid Desaturases/metabolism ; Fatty Acid Desaturases/genetics ; Fluorescence ; Seedlings/drug effects ; Seedlings/metabolism ; Seedlings/genetics ; Photosynthesis/drug effects |
| مستخلص: | Monogalactosyldiacylglycerol (MGDG) is the main lipid constituent of thylakoids and a structural component of photosystems and photosynthesis-related proteo-lipid complexes in green tissues. Previously reported changes in MGDG abundance upon stress treatments are hypothesized to reflect mobilization of MGDG-based polyunsaturated lipid intermediates to maintain extraplastidial membrane integrity. While exchange of lipid intermediates between compartmental membranes is well documented, physiological consequences of mobilizing an essential thylakoid lipid, such as MGDG, for an alternative purpose are not well understood. Arabidopsis seedlings exposed to mild (50 mM) salt treatment displayed significantly increased abundance of both MGDG and the extraplastidial lipid, phosphatidylcholine (PC). Interestingly, similar increases in MGDG and PC were observed in Arabidopsis fad3 mutant seedlings defective in endoplasmic reticulum (ER)-localized linolenic acid formation, in which compensatory plastid-to-ER-directed mobilization of linolenic acid-containing intermediates takes place. The postulated (salt) or evident (fad3) plastid-ER exchange of intermediates concurred with altered thylakoid function according to parameters of photosynthetic performance. While salt treatment of wild-type seedlings inhibited photosynthetic parameters in a dose-dependent manner, interestingly, untreated fad3 mutants did not show overall reduced photosynthetic quantum yield. By contrast, we observed a reduction specifically of non-photochemical quenching (NPQ) under high light, representing only part of observed salt effects. The decreased NPQ in the fad3 mutant was accompanied by reduced activity of the xanthophyll cycle, leading to a reduced concentration of the NPQ-effective pigment zeaxanthin. The findings suggest that altered ER-located fatty acid unsaturation and ensuing inter-organellar compensation impacts on the function of specific thylakoid enzymes, rather than globally affecting thylakoid function. (© The Author(s) 2023. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | 400681449/GRK2498 TP01 to I.H. 400681449/GRK2498 TP09 to K.H. Deutsche Forschungsgemeinschaft |
| فهرسة مساهمة: | Keywords: Arabidopsis thaliana; Membrane lipids; Monogalactosyldiacylglycerol; NPQ; Phosphatidylcholine; Xanthophyll cycle |
| المشرفين على المادة: | 0RBV727H71 (alpha-Linolenic Acid) 0 (Arabidopsis Proteins) 0 (Galactolipids) 1406-65-1 (Chlorophyll) 0 (Xanthophylls) 0 (monogalactosyldiacylglycerol) EC 1.14.99.- (fad3 protein, Arabidopsis) EC 1.14.19.- (Fatty Acid Desaturases) |
| تواريخ الأحداث: | Date Created: 20231122 Date Completed: 20240627 Latest Revision: 20240718 |
| رمز التحديث: | 20240718 |
| DOI: | 10.1093/pcp/pcad141 |
| PMID: | 37991227 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1471-9053 |
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| DOI: | 10.1093/pcp/pcad141 |