دورية أكاديمية
أعرض في EDS

ILF3 safeguards telomeres from aberrant homologous recombination as a telomeric R-loop reader.

التفاصيل البيبلوغرافية
العنوان: ILF3 safeguards telomeres from aberrant homologous recombination as a telomeric R-loop reader.
المؤلفون: Wang C; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China.; Department of Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510275, China., Huang Y; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China., Yang Y; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China.; Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China., Li R; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China., Li Y; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China., Qiu H; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China., Wu J; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China., Shi G; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China., Ma W; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China., Songyang Z; MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol and Guangzhou Key Laboratory of Healthy Aging, School of Lifesciences, Sun Yat-sen University, Guangzhou 510275, China.; Department of Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510275, China.
المصدر: Protein & cell [Protein Cell] 2024 Jul 01; Vol. 15 (7), pp. 493-511.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: Germany NLM ID: 101532368 Publication Model: Print Cited Medium: Internet ISSN: 1674-8018 (Electronic) Linking ISSN: 1674800X NLM ISO Abbreviation: Protein Cell Subsets: MEDLINE
أسماء مطبوعة: Publication: 2023- : [Oxford] : Oxford University Press
Original Publication: Heidelberg : Beijing, China : Springer ; Higher Education Press
مواضيع طبية MeSH: Telomere*/metabolism , Telomere*/genetics , Nuclear Factor 90 Proteins*/metabolism , Nuclear Factor 90 Proteins*/genetics , Homologous Recombination* , R-Loop Structures*/genetics, Humans ; DEAD-box RNA Helicases/metabolism ; DEAD-box RNA Helicases/genetics ; HEK293 Cells ; Telomere Homeostasis
مستخلص: Telomeres are specialized structures at the ends of linear chromosomes that protect genome stability. The telomeric repeat-containing RNA (TERRA) that is transcribed from subtelomeric regions can invade into double-stranded DNA regions and form RNA:DNA hybrid-containing structure called R-loop. In tumor cells, R-loop formation is closely linked to gene expression and the alternative lengthening of telomeres (ALT) pathway. Dysregulated R-loops can cause stalled replication forks and telomere instability. However, how R-loops are recognized and regulated, particularly at telomeres, is not well understood. We discovered that ILF3 selectively associates with telomeric R-loops and safeguards telomeres from abnormal homologous recombination. Knocking out ILF3 results in excessive R-loops at telomeres and triggers telomeric DNA damage responses. In addition, ILF3 deficiency disrupts telomere homeostasis and causes abnormalities in the ALT pathway. Using the proximity-dependent biotin identification (BioID) technology, we mapped the ILF3 interactome and discovered that ILF3 could interact with several DNA/RNA helicases, including DHX9. Importantly, ILF3 may aid in the resolution of telomeric R-loops through its interaction with DHX9. Our findings suggest that ILF3 may function as a reader of telomeric R-loops, helping to prevent abnormal homologous recombination and maintain telomere homeostasis.
(© The Author(s) 2023. Published by Oxford University Press on behalf of Higher Education Press.)
References: Curr Biol. 2003 Sep 2;13(17):1549-56. (PMID: 12956959)
Cell Rep. 2018 May 8;23(6):1891-1905. (PMID: 29742442)
Nature. 2020 Nov;587(7833):303-308. (PMID: 33057192)
Biochem J. 2018 Aug 31;475(16):2667-2679. (PMID: 30045876)
Cell Death Differ. 2021 Mar;28(3):1062-1075. (PMID: 33082515)
Nat Commun. 2017 Apr 21;8:15025. (PMID: 28429714)
Cell Res. 2020 Feb;30(2):163-178. (PMID: 31772275)
Cell. 2017 Jun 29;170(1):86-101.e16. (PMID: 28666128)
Methods. 2017 Feb 1;114:39-45. (PMID: 27530378)
Science. 1994 Dec 23;266(5193):2011-5. (PMID: 7605428)
Sci Rep. 2017 Mar 03;7:43650. (PMID: 28255170)
Nucleic Acids Res. 2019 Jan 8;47(D1):D1211-D1217. (PMID: 30252093)
Cell. 2021 Mar 4;184(5):1314-1329.e10. (PMID: 33626331)
Cell. 2018 Oct 4;175(2):558-570.e11. (PMID: 30245011)
Aging (Albany NY). 2012 Oct;4(10):695-708. (PMID: 23117626)
Nat Cell Biol. 2008 Feb;10(2):228-36. (PMID: 18157120)
J Biol Chem. 2004 Dec 3;279(49):51338-42. (PMID: 15383534)
J Biol Chem. 1994 Aug 12;269(32):20682-90. (PMID: 8051169)
Nat Struct Mol Biol. 2015 Nov;22(11):875-80. (PMID: 26581522)
Wiley Interdiscip Rev RNA. 2015 Mar-Apr;6(2):243-56. (PMID: 25327818)
FEBS Lett. 2005 Feb 7;579(4):859-62. (PMID: 15680963)
Clin Cancer Res. 2005 Jan 1;11(1):217-25. (PMID: 15671549)
Mol Cell. 2017 Jul 20;67(2):214-227.e7. (PMID: 28625552)
Mol Cell. 2020 Aug 6;79(3):425-442.e7. (PMID: 32615088)
Nat Struct Mol Biol. 2018 Feb;25(2):147-153. (PMID: 29358759)
Cell. 2017 Jun 29;170(1):72-85.e14. (PMID: 28666126)
Cancer Res. 1999 Sep 1;59(17):4175-9. (PMID: 10485449)
Am J Hum Genet. 2017 Nov 2;101(5):716-724. (PMID: 29100085)
Nucleic Acids Res. 2012 Aug;40(14):6649-59. (PMID: 22553368)
Genes Dev. 2005 Sep 15;19(18):2100-10. (PMID: 16166375)
Mol Cell Biol. 2010 Jan;30(1):146-59. (PMID: 19841062)
Biochim Biophys Acta Gen Subj. 2017 May;1861(5 Pt B):1371-1381. (PMID: 27913192)
Nucleic Acids Res. 2021 Sep 7;49(15):8573-8591. (PMID: 34329467)
J Biol Chem. 1999 Dec 3;274(49):34598-604. (PMID: 10574923)
Genetics. 2013 Aug;194(4):847-57. (PMID: 23733785)
Nat Commun. 2020 Sep 10;11(1):4534. (PMID: 32913330)
Nature. 2023 Jan;613(7942):187-194. (PMID: 36544021)
Sci Adv. 2020 Jun 10;6(24):eaba0777. (PMID: 32704541)
Nat Cell Biol. 2023 May;25(5):754-764. (PMID: 37037994)
Nucleic Acids Res. 2020 Jun 19;48(11):6019-6031. (PMID: 32379321)
Cell. 1994 Jun 3;77(5):621-3. (PMID: 8205611)
Nucleic Acids Res. 2019 Sep 19;47(16):8424-8438. (PMID: 31251802)
Nat Struct Mol Biol. 2009 Dec;16(12):1244-51. (PMID: 19935685)
Nat Biotechnol. 2009 Dec;27(12):1181-5. (PMID: 19935656)
PLoS Genet. 2010 Jun 10;6(6):e1000986. (PMID: 20548962)
Nature. 1989 Jan 26;337(6205):331-7. (PMID: 2463488)
Cancer Res. 2023 May 2;83(9):1476-1489. (PMID: 36264156)
Nucleic Acids Res. 2020 Feb 20;48(3):1285-1300. (PMID: 31777915)
J Immunol. 2021 Jun 15;206(12):2949-2965. (PMID: 34031149)
Nature. 2016 Nov 3;539(7627):54-58. (PMID: 27760120)
Bioinformatics. 2012 Jul 1;28(13):1805-6. (PMID: 22543366)
Cell Discov. 2017 Sep 26;3:17034. (PMID: 28955502)
Nat Commun. 2014 Oct 21;5:5220. (PMID: 25330849)
Cell Rep. 2019 Sep 17;28(12):3167-3181.e6. (PMID: 31533039)
Nat Protoc. 2016 Dec;11(12):2301-2319. (PMID: 27809316)
Cancer Res. 2004 Apr 1;64(7):2324-7. (PMID: 15059879)
Cell Rep. 2018 Feb 13;22(7):1849-1860. (PMID: 29444436)
Nat Commun. 2021 Dec 16;12(1):7314. (PMID: 34916496)
J Cell Biol. 2012 Mar 19;196(6):801-10. (PMID: 22412018)
Science. 2007 Nov 2;318(5851):798-801. (PMID: 17916692)
EMBO J. 2017 Dec 1;36(23):3532-3547. (PMID: 29074626)
Nat Commun. 2017 Feb 08;8:14334. (PMID: 28176777)
Nature. 2019 Sep;573(7772):144-148. (PMID: 31435012)
J Biol Chem. 1994 Aug 12;269(32):20691-9. (PMID: 7519613)
Mol Cell Biol. 2008 Jan;28(2):772-83. (PMID: 18039850)
Nat Rev Genet. 2019 May;20(5):299-309. (PMID: 30760854)
Nature. 2011 May 19;473(7347):337-42. (PMID: 21593866)
J Immunol Methods. 1986 May 1;89(1):123-30. (PMID: 2422282)
Nat Commun. 2019 Mar 1;10(1):1001. (PMID: 30824709)
Nat Commun. 2018 Apr 18;9(1):1548. (PMID: 29670078)
Nucleic Acids Res. 2012 Apr;40(7):2995-3005. (PMID: 22139915)
Nucleic Acids Res. 2018 Nov 30;46(21):11592-11604. (PMID: 30256975)
Genome Res. 2018 Sep;28(9):1405-1414. (PMID: 30108179)
J Biol Chem. 1999 Jul 16;274(29):20432-7. (PMID: 10400669)
Nucleic Acids Res. 2007;35(18):6249-58. (PMID: 17855395)
Cell. 2014 Sep 25;159(1):108-121. (PMID: 25259924)
Nature. 2021 Jun;594(7862):283-288. (PMID: 33981036)
Nat Struct Mol Biol. 2017 Dec;24(12):1124-1131. (PMID: 29106411)
Nature. 1990 May 31;345(6274):458-60. (PMID: 2342578)
Nat Commun. 2018 Feb 7;9(1):533. (PMID: 29416069)
Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):3377-82. (PMID: 24550456)
معلومات مُعتمدة: 82271598, 81871109, 82071587, 31930058, 32330023 and 32170757 National Natural Science Foundation; 2018YFA0107003 National Key Research and Development Program of China; 2020A1515010462 Guang Dong Basic and Applied Basic Research Foundation
فهرسة مساهمة: Keywords: ILF3; RNA:DNA hybrids; homologous recombination; telomeric DNA damage responses; telomeric R-loops
المشرفين على المادة: 0 (Nuclear Factor 90 Proteins)
0 (ILF3 protein, human)
EC 3.6.4.13 (DEAD-box RNA Helicases)
تواريخ الأحداث: Date Created: 20231122 Date Completed: 20240630 Latest Revision: 20240722
رمز التحديث: 20240722
مُعرف محوري في PubMed: PMC11214836
DOI: 10.1093/procel/pwad054
PMID: 37991243
قاعدة البيانات: MEDLINE
الوصف
تدمد:1674-8018
DOI:10.1093/procel/pwad054