Imaging the Bacterial Cell Wall Using N -Acetyl Muramic Acid-Derived Positron Emission Tomography Radiotracers.

التفاصيل البيبلوغرافية
العنوان:	Imaging the Bacterial Cell Wall Using N -Acetyl Muramic Acid-Derived Positron Emission Tomography Radiotracers.
المؤلفون:	Lee SH; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Kim JM; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., López-Álvarez M; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Wang C; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Sorlin AM; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Bobba KN; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Pichardo-González PA; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Blecha J; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Seo Y; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States., Flavell RR; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States.; UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, California 94158, United States.; Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California 94158, United States., Engel J; Department of Medicine, University of California, San Francisco, San Francisco, California 94158, United States.; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California 94158, United States., Ohliger MA; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States.; Department of Radiology, Zuckerberg San Francisco General Hospital, San Francisco, California 94110, United States., Wilson DM; Department of Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, California 94158, United States.
المصدر:	ACS sensors [ACS Sens] 2023 Dec 22; Vol. 8 (12), pp. 4554-4565. Date of Electronic Publication: 2023 Nov 22.
نوع المنشور:	Journal Article; Research Support, N.I.H., Extramural
اللغة:	English
بيانات الدورية:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Washington, DC : American Chemical Society, [2016]-
مواضيع طبية MeSH:	Muramic Acids* , Peptidoglycan*, Humans ; Positron-Emission Tomography/methods ; Fluorine Radioisotopes ; Bacteria ; Cell Wall
مستخلص:	Imaging infections in patients is challenging using conventional methods, motivating the development of positron emission tomography (PET) radiotracers targeting bacteria-specific metabolic pathways. Numerous techniques have focused on the bacterial cell wall, although peptidoglycan-targeted PET tracers have been generally limited to the short-lived carbon-11 radioisotope ( t 1/2 = 20.4 min). In this article, we developed and tested new tools for infection imaging using an amino sugar component of peptidoglycan, namely, derivatives of N -acetyl muramic acid (NAM) labeled with the longer-lived fluorine-18 ( t 1/2 = 109.6 min) radioisotope. Muramic acid was reacted directly with 4-nitrophenyl 2-[ ¹⁸ F]fluoropropionate ([ ¹⁸ F]NFP) to afford the enantiomeric NAM derivatives ( S )-[ ¹⁸ F]FMA and ( R )-[ ¹⁸ F]FMA. Both diastereomers were easily isolated and showed robust accumulation by human pathogens in vitro and in vivo, including Staphylococcus aureus . These results form the basis for future clinical studies using fluorine-18-labeled NAM-derived PET radiotracers.
معلومات مُعتمدة:	R01 EB030897 United States EB NIBIB NIH HHS; S10 OD034286 United States OD NIH HHS; R21 AI164684 United States AI NIAID NIH HHS; R01 EB025985 United States EB NIBIB NIH HHS; R01 EB024014 United States EB NIBIB NIH HHS
فهرسة مساهمة:	Keywords: N-acetyl muramic acid; fluorine-18; infection imaging; peptidoglycan; positron emission tomography
المشرفين على المادة:	GZ5I74KB8G (Fluorine-18) 246FXU111L (N-acetylmuramic acid) 0 (Muramic Acids) 0 (Peptidoglycan) 0 (Fluorine Radioisotopes)
تواريخ الأحداث:	Date Created: 20231122 Date Completed: 20231225 Latest Revision: 20240618
رمز التحديث:	20240618
مُعرف محوري في PubMed:	PMC10749472
DOI:	10.1021/acssensors.3c01477
PMID:	37992233
قاعدة البيانات:	MEDLINE

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الوصف
تدمد:	2379-3694
DOI:	10.1021/acssensors.3c01477