دورية أكاديمية
A novel monoclonal antibody against porcine macrophage colony-stimulating factor (CSF1) detects expression on the cell surface of macrophages.
| العنوان: | A novel monoclonal antibody against porcine macrophage colony-stimulating factor (CSF1) detects expression on the cell surface of macrophages. |
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| المؤلفون: | Waddell LA; The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK., Wu Z; The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK., Sauter KA; The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK., Hope JC; The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK. Electronic address: Jayne.Hope@roslin.ed.ac.uk., Hume DA; Mater Research Institute-University of Queensland, 37 Kent St, Woolloongabba, Qld 4104, Australia. |
| المصدر: | Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2023 Dec; Vol. 266, pp. 110681. Date of Electronic Publication: 2023 Nov 18. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Country of Publication: Netherlands NLM ID: 8002006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2534 (Electronic) Linking ISSN: 01652427 NLM ISO Abbreviation: Vet Immunol Immunopathol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] Elsevier Scientific. |
| مواضيع طبية MeSH: | Macrophage Colony-Stimulating Factor* , Antibodies, Monoclonal*, Swine ; Mice ; Animals ; Humans ; Macrophages ; Monocytes ; Mononuclear Phagocyte System/metabolism |
| مستخلص: | Macrophage colony-stimulating factor (CSF1) controls the proliferation and differentiation of cells of the mononuclear phagocyte system through binding to the receptor CSF1R. The expression and function of CSF1 has been well-studied in rodents and humans, but knowledge is lacking in other veterinary species. The development of a novel mouse anti-porcine CSF1 monoclonal antibody (mAb) facilitates the characterisation of this growth factor in pigs. Cell surface expression of CSF1 was confirmed on differentiated macrophage populations derived from blood and bone marrow monocytes, and on lung resident macrophages, the first species for this to be confirmed. However, monocytes isolated from blood and bone marrow lacked CSF1 expression. This species-specific mAb delivers the opportunity to further understanding of porcine myeloid cell biology. This is not only vital for the role of pigs as a model for human health, but also as a veterinary species of significant economic and agricultural importance. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CSF1; Macrophage; Monoclonal antibodies; Pig; Porcine |
| المشرفين على المادة: | 81627-83-0 (Macrophage Colony-Stimulating Factor) 0 (Antibodies, Monoclonal) |
| تواريخ الأحداث: | Date Created: 20231122 Date Completed: 20231216 Latest Revision: 20231216 |
| رمز التحديث: | 20231218 |
| DOI: | 10.1016/j.vetimm.2023.110681 |
| PMID: | 37992576 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2534 |
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| DOI: | 10.1016/j.vetimm.2023.110681 |