دورية أكاديمية
Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation.
| العنوان: | Identifying biomarkers of pollutant exposure in ocean sentinels: Characterisation and optimisation of B-esterases in plasma from loggerhead turtles undergoing rehabilitation. |
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| المؤلفون: | Omedes S; Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, 08003, Barcelona, Spain., Crespo-Picazo JL; Fundació Oceanogràfic de la Comunitat Valenciana, Gran Vía Marques del Turia 19, 46005, Valencia, Spain., Robinson NJ; Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, 08003, Barcelona, Spain; Fundació Oceanogràfic de la Comunitat Valenciana, Gran Vía Marques del Turia 19, 46005, Valencia, Spain., García-Párraga D; Fundació Oceanogràfic de la Comunitat Valenciana, Gran Vía Marques del Turia 19, 46005, Valencia, Spain., Sole M; Institut de Ciències del Mar, CSIC, Pg. Marítim de la Barceloneta 37-49, 08003, Barcelona, Spain. Electronic address: msole@icm.csic.es. |
| المصدر: | Chemosphere [Chemosphere] 2024 Jan; Vol. 348, pp. 140770. Date of Electronic Publication: 2023 Nov 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Ltd Country of Publication: England NLM ID: 0320657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1298 (Electronic) Linking ISSN: 00456535 NLM ISO Abbreviation: Chemosphere Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Science Ltd Original Publication: Oxford, New York, : Pergamon Press. |
| مواضيع طبية MeSH: | Environmental Pollutants* , Turtles*, Animals ; Carboxylesterase ; Acetylcholinesterase ; Biomarkers ; Oceans and Seas |
| مستخلص: | Sea turtles are frequently proposed as indicator species for assessing ocean health. To faciliate the use of these species as bioindicators requires the development of tools for rapidly and effectively assessing individual health. Here, we collected 104 blood samples from 69 loggerhead sea turtles, Caretta caretta, undergoing rehabilitation to determine the connection between health status, the activity of B-esterases, and other biochemical parameters. To determine the optimal assay protocol for B-esterases, we measured the activity and kinetics of cholinesterases-(ChEs) and carboxylesterases (CEs) using 3 and 5 commercial substrates, respectively, at different assay conditions. IC50 values for the activity of B-esterases were calculated within a concentration range for model pesticide inhibitors. Turtles' health status was determined via routine veterinary procedures. During rehabilitation (which was associated with improving health status), we observed a decrease in the activity of most enzymes (especially in acetylcholinesterase) alongside an increase in CE when using p-nitrophenyl acetate as a substrate. As such, it is possible that the activity rates of plasmatic B-esterases could serve as an indicator of health status. There is also high potential that B-esterases could be specifically sensitive to marine pollutants although to further validate this would require future studies to specifically correlate B-esterarse activities to pollutant concentrations in blood or excreta. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Carboxylesterases; Caretta caretta; Cholinesterases; Marine litter; Plastic additives; Sea turtles |
| المشرفين على المادة: | 0 (Environmental Pollutants) EC 3.1.1.1 (Carboxylesterase) EC 3.1.1.7 (Acetylcholinesterase) 0 (Biomarkers) |
| تواريخ الأحداث: | Date Created: 20231122 Date Completed: 20231205 Latest Revision: 20231205 |
| رمز التحديث: | 20231205 |
| DOI: | 10.1016/j.chemosphere.2023.140770 |
| PMID: | 37992900 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1298 |
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| DOI: | 10.1016/j.chemosphere.2023.140770 |