دورية أكاديمية
أعرض في EDS

Constant fed-batch cultivation with glucose and propionate as co-substrate: A strategy to fine-tune polyhydroxyalkanoates monomeric composition in Pseudomonas spp.

التفاصيل البيبلوغرافية
العنوان: Constant fed-batch cultivation with glucose and propionate as co-substrate: A strategy to fine-tune polyhydroxyalkanoates monomeric composition in Pseudomonas spp.
المؤلفون: Santos-Oliveira PH; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; iAMB - Institute of Applied Microbiology, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany., Silva JGP; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Blank LM; iAMB - Institute of Applied Microbiology, ABBt - Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany., Silva LF; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Gomez JGC; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: jgcgomez@usp.br.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jan; Vol. 256 (Pt 2), pp. 128287. Date of Electronic Publication: 2023 Nov 21.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Polyhydroxyalkanoates*/metabolism , Pseudomonas putida*/metabolism, Pseudomonas/genetics ; Pseudomonas/metabolism ; Propionates/metabolism ; Glucose/metabolism
مستخلص: Pseudomonas sp. LFM693 is a 2-methylisocitrate lyase (prpB) disrupted mutant. This enzyme catalyzes a step in the 2-methylcitrate cycle, the only known and described pathway for propionate oxidation in this organism. The affected mutants can efficiently produce PHA containing even and odd-chain length hydroxyalkanoates (HA even/odd ) in the presence of propionate and glucose. In this study, a constant fed-batch configuration was utilized to control the composition of PHA and decrease the toxicity of propionate. The incorporation of HA odd into the copolymer was linear, ranging from 7 to approximately 30 %, and correlated directly with the propionate/glucose molar ratio in the feeding solution. This allowed for the molecular composition of the mclPHA to be fine-tuned with minimum process monitoring and control. The average PHA content was 52 % cell dry weight with a molar composition that favored 3-hydroxyalkanoates containing C8, C9, and C10. The conversion factor of propionate to HA odd varied between 0.36 and 0.53 mol·mol -1 (Y HAodd/prop .), which are significantly lower than the theoretical maximum efficiency (1.0 mol·mol -1 ). These results along with the lack of 2-methylisocitrate as a byproduct provides further support for the evidence that the mutant prpB - is still capable of oxidizing propionate.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Polyhydroxyalkanoates; Propionate; Tailored bioplastics
المشرفين على المادة: 0 (Propionates)
0 (Polyhydroxyalkanoates)
IY9XDZ35W2 (Glucose)
تواريخ الأحداث: Date Created: 20231123 Date Completed: 20240118 Latest Revision: 20240118
رمز التحديث: 20240118
DOI: 10.1016/j.ijbiomac.2023.128287
PMID: 37995793
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2023.128287