دورية أكاديمية
أعرض في EDS

Platelet and mitochondrial RNA is decreased in plasma-derived extracellular vesicles in women with preeclampsia-an exploratory study.

التفاصيل البيبلوغرافية
العنوان: Platelet and mitochondrial RNA is decreased in plasma-derived extracellular vesicles in women with preeclampsia-an exploratory study.
المؤلفون: Lekva T; Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway. tove.lekva@ous-research.no., Sundaram AYF; Department Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway., Roland MCP; Department of Obstetrics, Oslo University Hospital, Oslo, Norway., Åsheim J; Department Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway., Michelsen AE; Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway.; Faculty of Medicine, University of Oslo, Oslo, Norway., Norwitz ER; Tufts University School of Medicine, Boston, MA, USA., Aukrust P; Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway.; Faculty of Medicine, University of Oslo, Oslo, Norway.; Section of Clinical Immunology and Infectious Diseases, Oslo University Hospital, Oslo, Norway., Gilfillan GD; Department Medical Genetics, Oslo University Hospital and University of Oslo, Oslo, Norway., Ueland T; Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway.; Faculty of Medicine, University of Oslo, Oslo, Norway.; K. G. Jebsen Thrombosis Research and Expertise Center, University of Tromsø, Tromsø, Norway.
المصدر: BMC medicine [BMC Med] 2023 Nov 23; Vol. 21 (1), pp. 458. Date of Electronic Publication: 2023 Nov 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101190723 Publication Model: Electronic Cited Medium: Internet ISSN: 1741-7015 (Electronic) Linking ISSN: 17417015 NLM ISO Abbreviation: BMC Med Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central, 2003-
مواضيع طبية MeSH: Pre-Eclampsia*/genetics , RNA, Long Noncoding* , Extracellular Vesicles*/genetics , Extracellular Vesicles*/metabolism, Pregnancy ; Humans ; Female ; RNA, Mitochondrial/genetics ; RNA, Mitochondrial/metabolism ; Cohort Studies ; RNA, Messenger/metabolism ; Biomarkers/metabolism ; RNA, Transfer/genetics ; RNA, Transfer/metabolism
مستخلص: Background: Circulating extracellular vesicles (EVs) are increased in preeclampsia (PE) and are associated with severity and progression. We examined in this exploratory cohort study if the mRNAs and long noncoding RNAs (lncRNAs) in plasma-derived EVs were dysregulated in PE compared to normal pregnancy and display different temporal patterns during gestation.
Methods: We isolated EVs from plasma at weeks 22-24 and 36-38 in women with and without PE (n=7 in each group) and performed RNA-seq, focusing on mRNAs and lncRNAs. We validated highly expressed mitochondrial and platelet-derived RNAs discovered from central pathways in 60 women with/without PE. We examined further one of the regulated RNAs, noncoding mitochondrially encoded tRNA alanine (MT-TA), in leukocytes and plasma to investigate its biomarker potential and association with clinical markers of PE.
Results: We found abundant levels of platelet-derived and mitochondrial RNAs in EVs. Expression of these RNAs were decreased and lncRNAs increased in EVs from PE compared to without PE. These findings were further validated by qPCR for mitochondrial RNAs MT-TA, MT-ND2, MT-CYB and platelet-derived RNAs PPBP, PF4, CLU in EVs. Decreased expression of mitochondrial tRNA MT-TA in leukocytes at 22-24 weeks was strongly associated with the subsequent development of PE.
Conclusions: Platelet-derived and mitochondrial RNA were highly expressed in plasma EVs and were decreased in EVs isolated from women with PE compared to without PE. LncRNAs were mostly increased in PE. The MT-TA in leukocytes may be a useful biomarker for prediction and/or early detection of PE.
(© 2023. The Author(s).)
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فهرسة مساهمة: Keywords: Extracellular vesicles; Preeclampsia; lncRNA
المشرفين على المادة: 0 (RNA, Mitochondrial)
0 (RNA, Long Noncoding)
0 (RNA, Messenger)
0 (Biomarkers)
9014-25-9 (RNA, Transfer)
تواريخ الأحداث: Date Created: 20231123 Date Completed: 20231127 Latest Revision: 20240212
رمز التحديث: 20240212
مُعرف محوري في PubMed: PMC10666366
DOI: 10.1186/s12916-023-03178-x
PMID: 37996819
قاعدة البيانات: MEDLINE
الوصف
تدمد:1741-7015
DOI:10.1186/s12916-023-03178-x