دورية أكاديمية
أعرض في EDS

Topoisomerase 1 Activity Is Reduced in Response to Thermal Stress in Fruit Flies and in Human HeLa Cells.

التفاصيل البيبلوغرافية
العنوان: Topoisomerase 1 Activity Is Reduced in Response to Thermal Stress in Fruit Flies and in Human HeLa Cells.
المؤلفون: Juul-Kristensen T; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark., Keller JG; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark., Borg KN; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China., Hansen NY; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark., Foldager A; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark., Ladegaard R; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark., Ho YP; Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong SAR, China.; Centre for Biomaterials, The Chinese University of Hong Kong, Hong Kong SAR, China.; Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics, Hong Kong SAR, China.; State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong SAR, China., Loeschcke V; Department of Biology, Aarhus University, 8000 Aarhus, Denmark., Knudsen BR; Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.
المصدر: Biosensors [Biosensors (Basel)] 2023 Oct 24; Vol. 13 (11). Date of Electronic Publication: 2023 Oct 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Pub.
مواضيع طبية MeSH: Drosophila melanogaster*, Animals ; Humans ; HeLa Cells ; Biomarkers
مستخلص: In the modern world with climate changes and increasing pollution, different types of stress are becoming an increasing challenge. Hence, the identification of reliable biomarkers of stress and accessible sensors to measure such biomarkers are attracting increasing attention. In the current study, we demonstrate that the activity, but not the expression, of the ubiquitous enzyme topoisomerase 1 (TOP1), as measured in crude cell extracts by the REEAD sensor system, is markedly reduced in response to thermal stress in both fruit flies ( Drosophila melanogaster ) and cultivated human cells. This effect was observed in response to both mild-to-moderate long-term heat stress and more severe short-term heat stress in D. melanogaster . In cultivated HeLa cells a reduced TOP1 activity was observed in response to both cold and heat stress. The reduced TOP1 activity appeared dependent on one or more cellular pathways since the activity of purified TOP1 was unaffected by the utilized stress temperatures. We demonstrate successful quantitative measurement of TOP1 activity using an easily accessible chemiluminescence readout for REEAD pointing towards a sensor system suitable for point-of-care assessment of stress responses based on TOP1 as a biomarker.
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فهرسة مساهمة: Keywords: biomarker; biosensor; enzyme activity; thermal stress; topoisomerase 1
المشرفين على المادة: 0 (Biomarkers)
تواريخ الأحداث: Date Created: 20231124 Date Completed: 20231127 Latest Revision: 20231127
رمز التحديث: 20231127
مُعرف محوري في PubMed: PMC10669382
DOI: 10.3390/bios13110950
PMID: 37998125
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6374
DOI:10.3390/bios13110950