دورية أكاديمية
أعرض في EDS

Histologic and proteomic profile of two methods to decellularize human dental pulp tissue.

التفاصيل البيبلوغرافية
العنوان: Histologic and proteomic profile of two methods to decellularize human dental pulp tissue.
المؤلفون: Ribeiro VMDS; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil., Sousa MGDC; Knight Cancer Precision Biofabrication Hub, Knight Cancer Institute, Oregon Health and Science University, Portland, OR, United States; Cancer Early Detection Advanced Research Center, Oregon Health and Science University, Portland, OR, United States; Division of Biomaterials and Biomechanics, Department of Restorative Dentistry, School of Dentistry, Oregon Health & Science University, Portland, OR, United States., Garcia PR; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Departamento de Clínica Odontológica, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil., Santos LSD; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil., Duarte ECB; Curso de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil., Corrêa JR; Decanato de Ensino de Graduação, Universidade de Brasília, Brasília, Distrito Federal, Brazil., Labate CA; Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil., Cataldi TR; Laboratório Max Feffer de Genética de Plantas, Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, Brazil., da Costa RA; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil., Ramada MHS; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil., de Carvalho JL; Curso de Medicina, Universidade de Brasília, Brasília, Distrito Federal, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil., Rezende TMB; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, Distrito Federal, Brazil; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil; Departamento de Odontologia, Universidade de Brasília, Brasília, Distrito Federal, Brazil; Programa de Pós-Graduação em Odontologia, Universidade de Brasília, Brasília, Distrito Federal, Brazil. Electronic address: taia.rezende@unb.br.
المصدر: Archives of oral biology [Arch Oral Biol] 2024 Jan; Vol. 157, pp. 105847. Date of Electronic Publication: 2023 Nov 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Pergamon Press Country of Publication: England NLM ID: 0116711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1506 (Electronic) Linking ISSN: 00039969 NLM ISO Abbreviation: Arch Oral Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Oxford : Pergamon Press
Original Publication: Oxford, New York [etc.] Pergamon Press.
مواضيع طبية MeSH: Tissue Scaffolds*/chemistry , Dental Pulp*, Humans ; Proteomics ; Proteome/analysis ; Extracellular Matrix ; Tissue Engineering/methods
مستخلص: Objective: The present study compared the structural and proteomic architecture of extracellular matrices (ECM) of decellularized human dental pulp using two previously described protocols.
Design: Pulp tissue from 150 molars was extracted and three treatments took place, based on the Matoug-Elwerfelli Group (MG) and the Song Group (SG) protocols and an untreated pulp group (CG), to examine histoarchitecture and the effectiveness of the decellularization process, using histological analysis (n = 12) and scanning electron microscopy (SEM) (n = 3). Protein extraction took place using 100 mg dry weight of pulp, in triplicates for each group, and the shotgun proteome analysis was performed by nanoUPLC-MS E . Proteins were identified using the revised human UNIPROT database attached to the PLGS search engine.
Results: Histological analysis and SEM demonstrated that ECM in MG was more preserved. Proteome analysis showed that the decellularized process in MG maintained approximately 69.56% of proteins identified in untreated pulp tissue while SG maintained 28.26%.
Conclusions: ECM appears to be suitable as a potential biological scaffold for pulp revascularization and regeneration procedures, especially those processed according to the Matoug-Elwerfelli protocol. This finding can collaborate to enhance clinical solutions for young permanent teeth that have suffered necrosis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Decellularization dental pulp protocols; Decellularized extracellular matrix; Guided tissue regeneration; Proteomics; Tissue scaffolds
المشرفين على المادة: 0 (Proteome)
تواريخ الأحداث: Date Created: 20231124 Date Completed: 20231205 Latest Revision: 20231205
رمز التحديث: 20231205
DOI: 10.1016/j.archoralbio.2023.105847
PMID: 38000129
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-1506
DOI:10.1016/j.archoralbio.2023.105847