دورية أكاديمية
Label-free and ultrasensitive electrochemical transferrin detection biosensor based on a glassy carbon electrode and gold nanoparticles.
| العنوان: | Label-free and ultrasensitive electrochemical transferrin detection biosensor based on a glassy carbon electrode and gold nanoparticles. |
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| المؤلفون: | Rabbani G; IT-medical Fusion Center, 350-27 Gumidae-ro, Gumi-si, Gyeongbuk 39253, Republic of Korea., Khan ME; Department of Chemical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia. Electronic address: mekhan@jazanu.edu.sa., Khan AU; Department of Electrical Engineering Technology, College of Applied Industrial Technology, Jazan University, 45142, Saudi Arabia., Ali SK; Department of Chemistry, Faculty of Science, Jazan University, Jazan, PO Box 114, Saudi Arabia., Zamzami MA; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21452, Saudi Arabia., Ahmad A; Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21452, Saudi Arabia., Bashiri AH; Department of Mechanical Engineering, College of Engineering, Jazan University, P. O. Box 114, Jazan 45142, Saudi Arabia., Zakri W; Department of Mechanical Engineering, College of Engineering, Jazan University, P. O. Box 114, Jazan 45142, Saudi Arabia. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2024 Jan; Vol. 256 (Pt 1), pp. 128312. Date of Electronic Publication: 2023 Nov 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Metal Nanoparticles*/chemistry , Biosensing Techniques*/methods , Metal Metabolism, Inborn Errors*, Carbon/chemistry ; Gold/chemistry ; Transferrin ; Electrodes ; Electrochemical Techniques/methods ; Limit of Detection |
| مستخلص: | In this study, we developed a label-free and ultrasensitive electrochemical biosensor for the detection of transferrin (Tf), an important serum biomarker of atransferrinemia. The biosensor was fabricated by using glassy carbon electrode (GCE) and modified with gold nanoparticles (AuNPs) via electroless deposition. The electrochemical characteristics of the GCE-AuNPs biosensors were characterized using cyclic voltammetry and electrochemical impedance spectroscopy analysis. Differential pulse voltammetry was used for quantitative evaluation of the Tf-antigen by recording the increase in the anodic peak current of GCE-AuNPs biosensor. The GCE-AuNPs biosensor demonstrates superior sensing performance for Tf-antigen fortified in buffer, with a wide linear range of 0.1 to 5000 μg/mL and a limit of detection of 0.18 μg/mL. The studied GCE-AuNPs biosensor showed excellent sensitivity, selectivity, long-term storage stability and simple sensing steps without pretreatment of clinical samples. This GCE-AuNPs biosensor indicates great potential for developing a Tf detection platform, which would be helpful in the early diagnosis of atransferrinemia. The developed GCE-AuNPs biosensor holds great potential in biomedical research related to point of care for the early diagnosis and monitoring of diseases associated with aberrant serum transferrin levels. These findings suggest that the GCE-AuNPs biosensor has great potential for detecting other serum biomarkers. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Glassy carbon electrode; Gold nanoparticles; Label-free electrochemical biosensor; Transferrin |
| المشرفين على المادة: | 7440-44-0 (Carbon) 7440-57-5 (Gold) 0 (Transferrin) |
| SCR Disease Name: | Congenital atransferrinemia |
| تواريخ الأحداث: | Date Created: 20231124 Date Completed: 20240118 Latest Revision: 20240118 |
| رمز التحديث: | 20240118 |
| DOI: | 10.1016/j.ijbiomac.2023.128312 |
| PMID: | 38000589 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2023.128312 |