دورية أكاديمية
أعرض في EDS

3D-printed TCP-HA scaffolds delivering MicroRNA-302a-3p improve bone regeneration in a mouse calvarial model.

التفاصيل البيبلوغرافية
العنوان: 3D-printed TCP-HA scaffolds delivering MicroRNA-302a-3p improve bone regeneration in a mouse calvarial model.
المؤلفون: Limlawan P; Department of Oral Medicine, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand.; Research Unit on Oral Microbiology and Immunology, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand., Insin N; Department of Chemistry, Faculty of Science, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand., Marger L; Biomaterials Laboratory, Division of Fixed Prosthodontics and Biomaterials, University Clinics of Dental Medicine, University of Geneva, 1 Rue Michel Servet, 1204, Geneva, Switzerland., Freudenreich M; Biomaterials Laboratory, Division of Fixed Prosthodontics and Biomaterials, University Clinics of Dental Medicine, University of Geneva, 1 Rue Michel Servet, 1204, Geneva, Switzerland., Durual S; Biomaterials Laboratory, Division of Fixed Prosthodontics and Biomaterials, University Clinics of Dental Medicine, University of Geneva, 1 Rue Michel Servet, 1204, Geneva, Switzerland., Vacharaksa A; Research Unit on Oral Microbiology and Immunology, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand. Anjalee.V@chula.ac.th.; Department of Microbiology, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand. Anjalee.V@chula.ac.th.; Master of Science Program in Geriatric Dentistry and Special Patients Care, Faculty of Dentistry, Chulalongkorn University, Pathumwan, Bangkok, 10330, Thailand. Anjalee.V@chula.ac.th.
المصدر: BDJ open [BDJ Open] 2023 Nov 24; Vol. 9 (1), pp. 50. Date of Electronic Publication: 2023 Nov 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Nature in partnership with British Dental Association Country of Publication: England NLM ID: 101709456 Publication Model: Electronic Cited Medium: Internet ISSN: 2056-807X (Electronic) Linking ISSN: 2056807X NLM ISO Abbreviation: BDJ Open Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: <2016-> : [London] : Springer Nature in partnership with British Dental Association
Original Publication: [London] : Nature Publishing Group publishes BDJ Open in partnership with the BDA, [2015]-
مستخلص: Objective: To demonstrate hydroxyapatite nanoparticles modified with cationic functional molecules. 3-aminopropyltriethoxysilane (HA-NPs-APTES) carrying microRNA-302a-3p (miR) in the 3D-printed tricalcium phosphate/Hydroxyapatite (TCP/HA) scaffold can increase healing of the critical-sized bone defect.
Materials and Methods: 3D-printed TCP/HA were modified with HA-NPs-APTES by two methods (M1, M2). The dispersion of particles was visualized by fluorescent microscopy. Biocompatibility of the scaffolds was tested by alizarin assay. Delivery of miR to the cells and osteogenic gene expression were evaluated by qPCR. After selecting best method (M2), scaffolds, scaffolds+HA-NPs-APTES with or without miR were implanted in 4 mm mouse calvarium defect (n = 4 per group). After 2,4 and 6 weeks, bone regeneration were evaluated by microCT and histology sections.
Results: Both M1 and M2 scaffolds were biocompatible with cell adhesion on its surface. M2 scaffold showed significant increase of miR, suggesting successful delivery, resulted in downregulation of its target mRNA COUP-TFII, and upregulation of RUNX2 mRNA. Calvarium defect with M2 scaffold also showed significantly higher BV/TV and higher number of filled spaces at all time points. Histomorphometry demonstrated new bone formed at the center of the HA-NPs-APTES-miR scaffold earlier than controls.
Conclusion: TCP/HA scaffold modified with HA-NPs-APTES facilitated delivery of miR and enhanced bone regeneration.
(© 2023. The Author(s).)
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تواريخ الأحداث: Date Created: 20231124 Latest Revision: 20231127
رمز التحديث: 20231127
مُعرف محوري في PubMed: PMC10673873
DOI: 10.1038/s41405-023-00177-1
PMID: 38001073
قاعدة البيانات: MEDLINE
الوصف
تدمد:2056-807X
DOI:10.1038/s41405-023-00177-1