دورية أكاديمية
أعرض في EDS

Exploring Viral Metagenomics in Pediatric Patients with Acute Respiratory Infections: Unveiling Pathogens beyond SARS-CoV-2.

التفاصيل البيبلوغرافية
العنوان: Exploring Viral Metagenomics in Pediatric Patients with Acute Respiratory Infections: Unveiling Pathogens beyond SARS-CoV-2.
المؤلفون: de Campos GM; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14050-190, Brazil., de La-Roque DGL; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14050-190, Brazil., Lima ARJ; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., Zucherato VS; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14050-190, Brazil., de Carvalho E; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., de Lima LPO; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., de Queiroz Cattony Neto P; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., Dos Santos MM; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., Ciccozzi M; Unit of Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, 00128 Rome, Italy., Giovanetti M; Instututo Rene Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Brazil.; Sciences and Technologies for Sustainable Development and One Health, Università Campus Bio-Medico di Roma, 00128 Rome, Italy., Haddad R; Campus Ceilândia, University of Brasília, Federal District of Brazil, Brasília 70910-900, Brazil., Alcantara LCJ; Instututo Rene Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Brazil., Elias MC; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., Sampaio SC; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., Covas DT; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil., Kashima S; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14050-190, Brazil., Slavov SN; Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto 14050-190, Brazil.; Center for Scientific Development (CDC), Butantan Institute, São Paulo 05503-900, Brazil.
المصدر: Microorganisms [Microorganisms] 2023 Nov 10; Vol. 11 (11). Date of Electronic Publication: 2023 Nov 10.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: The emergence of SARS-CoV-2 and the subsequent pandemic have prompted extensive diagnostic and clinical efforts to mitigate viral spread. However, these strategies have largely overlooked the presence of other respiratory viruses. Acute respiratory diseases in pediatric patients can be caused by a diverse range of viral agents, and metagenomics represents a powerful tool for their characterization. This study aimed to investigate the viral abundance in pediatric patients with acute respiratory symptoms who tested negative for SARS-CoV-2 during the Omicron pandemic wave. To achieve this, viral metagenomics and next-generation sequencing were employed on 96 nasopharyngeal swab samples, which were organized into 12 pools, with each pool consisting of eight individual samples. Metagenomic analysis revealed that the most prevalent viruses associated with acute disease in pediatric patients were respiratory syncytial virus (detected in all pools) and enteroviruses, which are known to cause significant morbidity and mortality in children. Additionally, clinically significant viruses such as mumps orthorubulavirus, human metapneumovirus, influenza A, and a wide array of human herpesviruses (1, 3-7) were identified. These findings highlight the extensive potential of viral metagenomics in identifying viruses other than SARS-CoV-2 that contribute to acute infections in children. Consequently, this methodology should garner clinical attention in terms of differential diagnosis and the development of public policies to address such conditions in the global pediatric population.
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معلومات مُعتمدة: 2022/00910-6; 2021/09446-8; 2021/11944-6 (CeVIVAS); 2018/15826-5; 2017/23205-8 São Paulo Research Foundation
فهرسة مساهمة: Keywords: acute infection; enteroviruses; next-generation sequencing; respiratory syncytial virus; respiratory viruses; viral metagenomics
تواريخ الأحداث: Date Created: 20231125 Latest Revision: 20231127
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10672962
DOI: 10.3390/microorganisms11112744
PMID: 38004755
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2607
DOI:10.3390/microorganisms11112744